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Questions related to Staining
I am planning to stain a cell surface protein by immunofluorescence and then take readouts by plate reader. As I would like to detect cell surface protein, I guess cells should not be fixed. I...
08 August 2023 9,637 5 View
The color of the mold colony morphology is teal (above) and white (below). Mold isolated from soil planted with soybeans. From the microscopic images it can be seen that this mold appears to be a...
03 August 2023 4,902 0 View
We've been looking at counting amyloid plaques in mouse hippocampus using 20x fluorescence images (antibody D5452). However when using either ImageJ or Matlab code, the fill holes or watershed...
01 August 2023 5,624 3 View
any scoring system available or a quantitively method?
30 July 2023 3,885 4 View
In the detection of programmed cell death, the literature shows by using Promega PCD kit, the TUNEL staining shows green fluorescence and DAPI staining shows blue fluorescence. I want to make it...
24 July 2023 7,831 9 View
Hi, I am currently using pHrodo Green AM as a marker for my target cells, which was used in a phagocytosis assay with my M1-like THP-1 cells and I have some questions regarding this specific...
18 July 2023 9,942 1 View
I am new to creating solutions, and any help would be beneficial!
18 July 2023 9,067 0 View
I am working on a project identifying molds grown on hard cheese. One of my plates (PDA) had growth that looked like yeast. I have been staining the different mold samples using LCPB. I attemped...
17 July 2023 602 1 View
Assuming that both are heat inactivated and sterile filtered, is there a difference between normal goat serum and donor goat serum. I can't seem to find any major difference between Is their an...
17 July 2023 4,157 2 View
Dear researchers, I am performing heart staining with TTC. after staining, the tissue becomes hard, rough and shrinks and i am unable to take photographs. How I can avoid it?
17 July 2023 5,003 1 View
Hi everyone. I have protein with concentration of 0.6 mg/mL The total volume of my protein is 4 mL. Protein size is ~18 kDa How can I convert my total protein concentration to micro...
11 July 2023 8,526 8 View
I have a problem with number of cells in E0771 cell line . I usually use 100-2000 cells for sphere but I couldn't get good sphere.
05 July 2023 1,061 6 View
I am doing westernblots. Thee independent experiments. In each experiment i get different result. For example, if i see a decrease in my target protein at 10ng/ml then in another repetition, it is...
04 July 2023 5,044 3 View
Here is an image I tried to take using TEM ( JEM-1400S) for human adenovirus. I used uranyl acetate to negatively stain the sample. However, this image I have indicates the sample is positively...
28 June 2023 421 1 View
I have found a Bacillus isolate that showed antimicrobial activity against gram positive and negative ATCC test strain. I have done the test three times where showed same activity against test...
24 June 2023 8,903 2 View
After staining, the sections appear very light. Should I increase the incubation time in DAB?
19 June 2023 622 1 View
Hi, I am looking for BAC or YAC that encode for the chromosome 21. Does anyone know where I could be able to find that? Have been looking around online but it seas as it is not available to...
13 June 2023 5,326 2 View
Planning to do IF stainning of paraffin embedded urothelial tissues from Göttingen minipigs. Any insights from previous experience is warmly welcomed!
12 June 2023 2,701 2 View
Hello everyone, I am trying to access TNFalpha cytotoxic effect on A549 cell line ( from 2.5-40ng/ml) using Invitrogen Live/Dead staining protocol (Calcein AM/ Ethidium homodimer-1). I found that...
07 June 2023 878 0 View
Hello! I need help making a 25 mM HEPES buffer with a pH of 7.4. I've tried calculating the dilution, but I keep messing up. The stock solution I am planning to use is: 30 mM HEPES Buffered...
05 June 2023 7,767 1 View
I urgently need a personal copy of CLSI M100 33rd Edition of Performance Standards for Antimicrobial Susceptibility Testing
04 June 2023 229 0 View
Hi, I encountered an issue while detaching some cells. I had both HEK 293 and RAW 264.7 cells. When I added 0.05% trypsin, the cells did not round up and instead detached in the form of a sheet....
02 June 2023 1,228 3 View
I am looking for all the possible solution could be implemented to remove sticky PDMS from master mould and with it any necessary precaution needed for avoiding similar situation as shown in the...
01 June 2023 4,687 3 View
I'm trying to do a long-range PCR (~10kb) and I'm just wondering what polymerase to use despite the Q5. Do you have any experience in doing a long-range PCR with other polymerases? Did you use the...
31 May 2023 9,712 3 View