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Questions related to Staining
Question: I am facing issues in the Ni-affinity chromatography step after His-tag removal (Post-His-tag removal purification). I loaded about 15 mL of my protein sample onto the column. I...
13 September 2025 8,934 4 View
How will I wash His Trap HP 5 ml column before and after using it for protein purification?
11 September 2025 4,071 2 View
I am performing protein purification on an ÄKTA Prime Plus system. After His-tag affinity purification, I cleave the His-tag from my protein with thrombin. In the post-His-tag removal step, I...
06 September 2025 5,379 3 View
(column bed is 5mL, column volume is 20mL) gravity flow
04 September 2025 3,980 3 View
I had done elisa of bmp2 of elder populations by ELABSCIENCE ELISA KIT by following standard protocol, but every time it shows that OD of serum samples are come lesser than blank means Zero...
25 July 2025 8,632 2 View
According to the ASTM method D4607-14, the contact time between iodine solution and activated carbon is specified as 30 ± 1 seconds. Is this duration scientifically valid and sufficient for...
24 June 2025 2,004 0 View
i want to know Background. methodology and laboratory experiment
01 June 2025 2,006 0 View
Las habilidades sociales en los niños se refieren a su capacidad para interactuar de forma efectiva y positiva con otras personas, incluyendo amigos, familiares y compañeros de clase. Las...
23 May 2025 2,025 3 View
Necesidad de saber la mejor forma de aprender para estudiante de primeros años en Medicina.
22 May 2025 9,191 0 View
Estudiante de Medicina que desea especializarle en Pediatría, pero subespecializarse en Neurología Pediátrica.
22 May 2025 3,672 0 View
Estudiante de Medicina Humana que desea especializarse en Dermatología
22 May 2025 3,262 1 View
Soy estudiante de Medicina Humana con especial interés en oncología y endocrinología. Me motiva entender cómo las vías hormonales pueden influir en el desarrollo y comportamiento de ciertos tipos...
22 May 2025 9,726 1 View
I am currently performing a kill curve with HEK-293 and OE-33 cells and geneticin. I followed the same protocol for both cell lines: 48 well plate. 8 diff concentrations (0 µg/ml -> 1000...
14 May 2025 5,789 2 View
Hello, everyone! When using Superdex 200 for size-exclusion chromatography, I observe three peaks: the first is >600 kDa, the second around 120 kDa, and the third around 30 kDa. My protein...
14 May 2025 2,454 4 View
The Trans-Atlantic slave trade involved the force transportation of approximately 12,5 million Africans to the Americas from the 16th century until the 1860s.
14 May 2025 4,592 1 View
Hi everyone, I have been using Percoll from Cytiva (https://www.cytivalifesciences.com/en/us/shop/cell-therapy/media/percoll-density-gradient-media-p-05823) to make density discontinuous...
13 May 2025 2,009 0 View
Good Day to respected readers. The Leica and Olympus microscopes, for slide-mounted acari, usually have either PC (phase contrast) and/or DIC (differential interference contrast). I am very much...
14 April 2025 673 0 View
Hi! I'd like to add a US Laboratory to my Profile, I already have one linked Laboratory with my-own being 'classified'; however, would like to include a second based in the USA. How do I add a...
11 April 2025 8,463 0 View
Dear Dr. Corrado Mollica, Hope my e-mail finds you well. I’m writing from conference organizing committee. Sorry to bother you. I just want to check if you have a plan to join our forthcoming...
28 March 2025 2,178 7 View
I need to measure Delamination using IMAGE processing in MATLAB. I have three composite sheet having 27 drilled holes. Contact me on my Whatsup no : 8849442944.
25 March 2025 8,979 1 View
Prostate cancer in situ modeling method, how to ensure uniformity, we have done it several times, the tumor size deviation is too large.
19 March 2025 264 2 View
9th Cοnfеrеnсе on Education and Information Technologies Dear C. Mollica, We extend our cordial іnνіtatіon to your esteemed presence at the 9th International Cоnfеrеnсе on Education and...
12 March 2025 6,609 0 View
We created a transgenic mouse line using the Cre-loxp system, allowing these mice to release fluorescent exosomes muscle-specifically by tagging turboGFP to CD63. Specific green fluorescence was...
04 March 2025 1,999 1 View
I want to analyze some spheroid images (CZI format). I have 3 images: each one shows spheroids of three different types of cells. Two of them are fibroblasts and are visible in the green channel....
02 March 2025 4,218 3 View