Staining

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Questions related to Staining

SMC and EC Co Culture Media ?

Does anyone have any suggestions on media, and ratio for smooth muscle cells and endothelial cells co culture? Thanks!

07 November 2022 9,290 2 View

Simulink CI using gitlab?

Hello, I need to use CI in gitlab for a simulink model. I haven't found anything on internet beside official math work site (they have one documentation about that). Have anyone worked with CI...

07 November 2022 7,141 0 View

Could any expert try to examine our novel approach for multi-objective optimization?

Could any expert try to examine our novel approach for multi-objective optimization? The brand new approch was entitled "Probability - based multi - objective optimization for material selection",...

06 November 2022 3,683 6 View

Does flow cytometry checking t cell cytokines from tumor-bearing mice need t cell activation?

I plan to check t cells from mouse tumors for IFN-y, TNF-a, IL-2 and granzyme B. Is T cell activation by PMA or anti-CD3/CD28 needed? Thanks!

01 November 2022 2,631 4 View

Why are my Western Blot transfers leaving so much protein behind in the gel?

*Before you comment with any of the generic troubleshooting advice from the front page of Google, just know I've already tried all that* No matter how I alter my conditions for wet tank transfer,...

31 October 2022 6,313 6 View

Is it possible to measure the contact perimeter/area between two different types of cells in ImageJ?

Hi everybody, I have colored manually different plant cell types, and I would like to measure the contact surface/perimeter between two types of cells. Do you know if I can do this using ImageJ?...

28 October 2022 6,268 4 View

Which MRI modality is best for the detection of brain tumors?

I have a brain MRI dataset which contains four image modalities: T1, T2, Flair and T1 contrast-enhanced. From this dataset, I want to segment the Non-Enhancing tumor core, Peritumoral Edema and...

26 October 2022 7,576 5 View

I have been working with PBMCs isolated from human blood. What are the weird things moving around in black?

I use bacterial cell wall components to stimulate the PBMCs. I see these weird things in all donors. the hood is sterile and the isolation very carefully. Any suggestions to what this could be and...

25 October 2022 8,719 2 View

How to prevent cells from dislodging from well plates for when changing media?

I am having trouble with C2C12 cells detaching from the well plates after adding conditioned media. Areas on the left and bottom sides of the wells lack cells after switching to the conditioned...

25 October 2022 9,800 6 View

Is there any suitable protocol for cell culture on the glass bottom plate?

Hello everyone. I am going to analyze differentiated cardiomyocytes (CM) via confocal microscopy. However, I struggle to attach CM properly to the glass slide/plate. Although I coated the glass...

24 October 2022 7,359 6 View

Has anyone used RNA cleanup by RNeasy kit after RNA extraction by TRIZOL?

Hello all, I am trying to isolate RNA from neuronal cells using Trizol ,chloroform, isopropanol. After reading the OD, the concentration of RNA is good, but their quality is not good. So, I...

24 October 2022 7,381 6 View

Weird result from Flowjo, why my G1 and G2 peak are far away from S phase?

Hi there, I would really appreciate some help. I have done the flow cytometry using this kit: BrdU Flow Kits http://www.icms.qmul.ac.uk/flowcytometry/uses/cellcycleanalysis/diagrams/brdu.pdf As...

23 October 2022 8,438 4 View

How to reduce holes on cryosectioning slides?

Hello, there are some holes/porous structure on my nissl staining results as shown in the picture below. Does it look like freezing defects or slides over dried? Some advice will be appreciate....

18 October 2022 4,707 7 View

Do I have to differentiate SH-SY5Y cells for Parkinson's disease model?

Hello everyone, I am using retinoic acid to differentiate SH-SY5Y cells into neuronal cells to generate a Parkinson's disease model. I will perform MTT assay before using a neurotoxin of interest...

16 October 2022 8,524 4 View

How important is serum-free medium for phagocytosis experiments?

Hello. Currently I am using the murine microglial cell line BV2 for phagocytosis experiments. I am wondering how key is the fact of treating the cells with serum-free medium. In what way affects...

14 October 2022 6,961 1 View

Does expired edita tube affect on Lymphocytes? For #FlowCytometry? Or rtpcr ?

I will use the samples for flow cytometry analysis and qpcr

11 October 2022 9,171 2 View

How to deal with alcian blue staining of parafin sections?

I would like to do the alcian blue staining of tissue-engineered cartilage. For this, I have embedded my samples in paraffin and cut them into 10um thick sections. During the deparaffinization...

10 October 2022 2,502 3 View

CAN YOU PLEASE SHARE ON THIS QUERY : What is the physiological basis of plant growth regulator induced hermaphrodite flowers in gynoecious plant?

Gynodioecious cucumber genotype shows induced hermaphrodite flowers post the plant growth regulators spray. What makes a gynoecious plant produce hermaphrodite flowers (fertile maleness in...

10 October 2022 1,028 0 View

How long can I leave the ELISA plate (R&D DuoSet ELISA mouse IL-1beta) in capture antibody?

I am planning to coat the plate with capture antibody over the weekend at 4 degree. I am not sure if that will make a difference than incubating overnight at room temperature which is what the...

07 October 2022 4,573 1 View

Does Paraformaldehyde degrade at room temperature?

Hello Researchers, I have been trying to do a BODIPY stain of lipid droplets in THP-1 cells without luck. The images are fuzzy and blurry at 63x magnification on confocal microscope. I had...

07 October 2022 9,492 2 View

Why can't I get images on western blot?

Hi everyone, I've tried western 5 times. But I can't get a picture. I loaded my proteins in 30 microliters, ladder 5 microliters. They progressed very well. I performed the transfer phase on ice....

02 October 2022 546 6 View

What is the best way to identify Treg from other CD4 T cells in human blood?

As I understand, Foxp3 staining is not ideal because it's intracellular and other CD4 can express Foxp3 transiently. Any markers can be used other than CD25?

30 September 2022 7,428 2 View

My instruments are (BD FACSMelody™ Cell Sorter) and Facscanto 2, My question is can i use APC with Percp.Cy5.5 ? would will have a spillover?

#Flowcytometery #flowjo #flowarea #Facscanto #flowcytometer

23 September 2022 5,929 0 View

How to find the similarity or dissimilarity among different patterns?

I have got different patterns after I treat my biosensor with different inhibitors dose and identity. (Attached are three patterns). Which method should I use to identify that these dynamic...

14 September 2022 5,349 0 View

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