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Questions related to Staining
Can anyone explain why a study's ELISpot assays might be designed wherein its cells are being plated at a concentration of 200K/well (the standard amount) and then 100K/well? The concentration of...
24 July 2023 5,512 1 View
Why is the rate of decomposition affected by pH of soil and hat environmental factors do decomposers require to increase their activity?
23 July 2023 1,870 2 View
I would like to transfect HEK293 with a plasmid containing a luciferase (downstream of a response element to this pathway) to check a cell signal pathway, but in order to have a population of...
21 July 2023 825 1 View
I want to analyze the following T regulatory cell phenotypes: CD4+CD25+IL-10+ T regs, CD4+CD25+Foxp3+ T regs, and CD4+LAP+ (TGF-beta1-secreting) T regs. The IL-10+ and LAP+ T regs seem to have to...
20 July 2023 907 0 View
Hi, I am currently using pHrodo Green AM as a marker for my target cells, which was used in a phagocytosis assay with my M1-like THP-1 cells and I have some questions regarding this specific...
18 July 2023 9,951 1 View
I am new to creating solutions, and any help would be beneficial!
18 July 2023 9,079 0 View
I am getting absorbance in 6 range nad transmittance in 6000 range even in the absence of sample. when sample is placed similar results obtained. In background mode only flat line at zero...
18 July 2023 2,335 3 View
I am working on a project identifying molds grown on hard cheese. One of my plates (PDA) had growth that looked like yeast. I have been staining the different mold samples using LCPB. I attemped...
17 July 2023 613 1 View
Assuming that both are heat inactivated and sterile filtered, is there a difference between normal goat serum and donor goat serum. I can't seem to find any major difference between Is their an...
17 July 2023 4,172 2 View
Dear researchers, I am performing heart staining with TTC. after staining, the tissue becomes hard, rough and shrinks and i am unable to take photographs. How I can avoid it?
17 July 2023 5,016 1 View
how to know about different types of contraction mapping to prove fixed point theorem like ciric, rus, f-contraction, suzuki contraction etc
16 July 2023 4,851 1 View
Is photosynthesis done by producers or consumers and bacteria that perform photosynthesis primary producers or consumers?
13 July 2023 5,702 6 View
Hi everyone. I have protein with concentration of 0.6 mg/mL The total volume of my protein is 4 mL. Protein size is ~18 kDa How can I convert my total protein concentration to micro...
11 July 2023 8,540 8 View
I will do qPCR from DNA genome. My DNA concentration is only 2-0.5 ng/uL. What is the minimum concentration of DNA template for qPCR? If need more DNA concentration, what should I do? Thank you...
07 July 2023 316 2 View
I have a problem with number of cells in E0771 cell line . I usually use 100-2000 cells for sphere but I couldn't get good sphere.
05 July 2023 1,075 6 View
I am doing westernblots. Thee independent experiments. In each experiment i get different result. For example, if i see a decrease in my target protein at 10ng/ml then in another repetition, it is...
04 July 2023 5,053 3 View
I tried ACN, DMSO and other solvent like methanol, water but its seems that peptide is not soluble and it is forming a gel in ACN and water. The peptide has 10-12 amino acid.
03 July 2023 7,300 1 View
Here is an image I tried to take using TEM ( JEM-1400S) for human adenovirus. I used uranyl acetate to negatively stain the sample. However, this image I have indicates the sample is positively...
28 June 2023 433 1 View
I have found a Bacillus isolate that showed antimicrobial activity against gram positive and negative ATCC test strain. I have done the test three times where showed same activity against test...
24 June 2023 8,913 2 View
How can preparation of sample for western blotting from isolated pancreatic islet be done?
24 June 2023 7,846 0 View
I'm using the R&D ELISA kit to read Lipocalin and Myeloperoxidase. The kit says to read at 450nm with correction of 540nm or 570nm but the Biotek 800TS absorbance reader only has 490nm and...
23 June 2023 6,531 0 View
After staining, the sections appear very light. Should I increase the incubation time in DAB?
19 June 2023 665 1 View
I'm using the Biotechne Human S100A8/S100A9 ELISA kit to assay fecal calprotectin but struggling to get the components needed for the recommended extraction buffer in the protocol of the kit: 0.1M...
19 June 2023 6,704 0 View
After extraction of folic acid from probiotic bacteria is there any further purification step?
16 June 2023 6,222 6 View