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Questions related to Staining
Does population size affect mutation and how can a mutation become more prevalent in certain populations?
24 October 2023 4,391 7 View
Hello, I am planning to write my first project where I will be attempting to find an optimal decalcification time for rodent (rats specifically) nasal turbinate's, in Kristensen fluid (KF). I was...
20 October 2023 993 0 View
Does anyone have tips for expanding a vector from a supplier? It's ~5ng/uL and stored in 50% glycerol. I attempted to do a transformation as normal but I did not get any growth.
19 October 2023 1,369 1 View
I want to cluster multiple pdb files with respect to one reference pdb. I have to use Align > all this to *Ca in Pymol. However, how could i do this via scripting in Pymol? As I have multiple...
16 October 2023 10,257 3 View
I make the skin longitudinal frozen section. leica machine I conduct the temperature -30℃，but when I start section the temperature will be -23~-27 ℃，this temperature mistake？ dont know why only...
16 October 2023 9,707 1 View
Can someone share their protocol for culture of primary human adrenal cortical cells please. Thank you in advance!
16 October 2023 7,150 0 View
Organ-on-a-Chip is an in vitro model for growing cells in microfluidic channels. The problem I am currently encountering is that Calu-3 cells cannot fill up my chip flow channel. I have tried cell...
16 October 2023 3,533 0 View
I use restriction enzyme cloning method, and I have been using new reagents e.g., competent cells, and ligase reagent because initially I thought the problem is that these reagents were outdated....
13 October 2023 9,814 10 View
2. In forest ecology studies, for which types of samples or datasets is the Margalef diversity index most applicable? Are there specific scenarios where it is particularly informative? 3. When...
13 October 2023 7,687 0 View
Dear Sir or Madam, could you investigate, why with my account i cannot send full-text using your on-line procedure to reply to requests? I'm using Google Chrome from my company site. Thanks and...
12 October 2023 491 1 View
I am using this protocol to stain intraepidermal nerve fibers in epidermis of a paraffin embedded 3 mm skin sections I first deparrafinized and hydrated tissues, applued citrate buffer ph 6...
11 October 2023 8,433 1 View
I made a recombinant plasmid and tested it by colony PCR and double restriction digest, both of them showed the construct was correct but the western blot always cannot show the protein I wanted...
09 October 2023 330 4 View
What is KIE's PH/PD value, and how is it used in catalysis?
06 October 2023 936 3 View
I urgently need those best image and figure scaning as well as developing softwares for my tsesis and paper write up and usually i have experience in using MS word,MS picture manager,MS power...
05 October 2023 4,002 8 View
Hi, I am currently running an experiment to determine the internalization of LDL with LDLR. I need to quantify my images. It's a bit difficult to quantify Dil-LDL since Dil-LDL appears as single...
04 October 2023 5,987 0 View
Hi! We are producing lentivirus for T cell transduction. However, there are several protocols that do not mention the use of polybrene for titration in HEK293T, but they use centrifugation...
04 October 2023 7,504 2 View
Ecologists should pay attention to which aspects when applying mathematics?
03 October 2023 4,957 8 View
Hi, looking for Foxp3-cre/GFP x R26-fl/YFP Treg lineage tracking mice on a BALB/c background. Does anyone have these or know of a lab that does? Thanks for your help!!!
02 October 2023 5,873 0 View
I am Performing analysis of Maltodextrin in Sugar Column by HPLC-RID. As i am getting slightly peak split even with new column. %RSD values of standard solutions and with bracketing standard...
30 September 2023 4,352 0 View
Waste sulfuric acid is a byproduct generated from isobutane/butene alkylation. It is essential to remove the impurities and recover the sulfuric acid from the waste acid.
29 September 2023 1,159 0 View
someone please explain what is the nature of histone binding to plasmid DNA in CYTOPLASM 1) Does histone bind in form of nucleosome to plasmid? or they float in the cytoplasm as individual...
28 September 2023 4,046 2 View
Network Pharmacology
26 September 2023 6,309 1 View
If I want to knock out or knock down an RNA binding protein, are there any specific considerations to keep in mind? How can I make sure that the wildtype reconstitution is able to bind RNA and...
25 September 2023 5,193 1 View
Has anyone worked with this antibody?
24 September 2023 4,727 0 View