Staining

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Questions related to Staining

QPCR Standard Curves showing unexpected signals?

I am currently generating a standard curve for two targets highly specific to my organism using a TaqMan probe-based qPCR assay and standard serial dilutions ranging from 10^6-10^1 gene copies. I...

28 November 2023 227 3 View

Can anyone recommended me best suited activation function in neural networks for real time scenarios?

Iam planning to do some literature work on rationale neural networks and functionalities of activation functions like sigmoid and others, please recommend me some effective articles related one

26 November 2023 1,152 3 View

How to draw a protein secondary structure topology from protein sequence?

Dear all, Can anyone provide me with a software or a webtool for drawing 2D-topology for a protein from a sequence similar to the one provided in the following figure?

17 November 2023 8,393 2 View

Why there is cytoplasmic stain of KI67 in HCC mouse liver tissue?

When stained with KI67 in mouse liver tissue with HCC, I can see cytoplasm stained and not nuclear staining. This is IF staining where the tissue is fixed with formalin and permeabilized with 0.2%...

15 November 2023 196 1 View

Importing model with CIN3D8 infinite Elements , removes existing sets! How to create node sets again with the resulting Orphan mesh?

I have created an Abaqus model using a Python script, the script creates the model, and defines the material, the assembly, the partitions, and the creation of sets of points, since I want to use...

15 November 2023 1,137 2 View

How to get the permission to use MARS-5 (Medication Adherence Report Scale-5) questionnaire for my research?

Hello everyone. I am planning to use the MARS-5 (Five item Medication Adherence Report Scale) questionnaire for my research. I want to know how should I get the permission to use this scale. Your...

13 November 2023 9,135 3 View

Is anyone currently using a programmable peristaltic pump with 4-roller pumping heads and 1/16" ID tubing from Braintree Scientific, Inc.?

I am in the process of setting up a programmable peristaltic pump for mouse cardiac perfusion. I'm uncertain about the appropriate blunt needle specifications, specifically 24 gauge (24g) with a...

13 November 2023 9,677 0 View

I'm trying to calculate a Soil Quality Index using the weighted additive SQI method. Do you have any R code that you can provide?

I'm trying to calculate a Soil Quality Index using the weighted additive SQI method. Do you have any R code that you can provide?

09 November 2023 505 2 View

How to isolate Golgi Vesicles from cultured cells?

I want to isolate Golgi vesicles from Hela cells by flotation through a discontinuous sucrose gradient. Theoretically, Golgi band will be harvested from the 0.8 M/1.2 M sucrose interface. But I...

03 November 2023 2,434 0 View

Hi, anyone working on cas12 protein for nucleic acid detection? I also wish to know how to design crRNA?

I was looking for nucleic acid detection with CRISPR. I wish to know how and where to design the crRNA? Which company deals with it? Any lead would be appreciated.

02 November 2023 510 1 View

Best way to preserve FACS enriched cells for downstream single nucleus RNA & ATAC seq?

I am working with a cell population that needs to be enriched by cell sorting from fresh tissue. I am wondering what the best way to preserve the cells, after sorting, for downstream single...

31 October 2023 9,178 0 View

How can deep learning models be optimized to improve the early detection of various cancer types using medical imaging data?

How can deep learning models be optimized to improve the early detection of various cancer types using medical imaging data?

31 October 2023 2,536 5 View

What strategies can be employed to overcome the limited availability of labeled data for training deep learning models in cancer identification?

What strategies can be employed to overcome the limited availability of labeled data for training deep learning models in cancer identification?

31 October 2023 4,005 3 View

What is the protocol to sonicate surgical instruments?

Hi, I'm exploring ways to sterilise my instruments used in cell culture to dissecte unfixed live human eye tissue. Does anyone have an ideal protocol for sonication or other sterilisation...

30 October 2023 4,595 2 View

Are there any ongoing collaborations within the realm of fixed point theory?

I am currently researching various contractions using a measure of noncompactness for both single and multivalued mappings. My study focuses on exploring the existence of solutions for equations...

30 October 2023 4,925 3 View

What is the purpose of Sephadex G-100 separation after PEG precipitation for exosomes/EVs in a publication?

Hello! I am reviewing a procedure for exosome/EV purification from a large-volume blood sample (porcine, terminal draw). In brief, protocol is: 1) Blood collection 2) Two-step spin to get...

27 October 2023 6,837 1 View

Nanoparticles impact on animal tissues: Histological (H&E) analysis of liver tissues, revealed the presence of slightly orange-colored cells?

Nanoparticles impact on animal tissues: Histological (H&E) analysis of liver tissues, revealed the presence of slightly orange-colored cells....... Does anybody observe similar structures in...

25 October 2023 5,229 3 View

Alcian blue staining protocol for glutaraldehyde-fixed chondrocytes?

I have fixed human chondrocytes in 6-well plate by glutaraldehyde. For alcian blue staining pH 1, I used this protocol: 1) Rinse wells 3x with PBS. 2) Rinse 1x with 1% acetic acid. 3) Apply...

25 October 2023 7,411 0 View

Annular lattice in MCNP?

Dear all. I need to describe an annular lattice in MCNP. I found the way to create a hexagonal lattice or rectangular lattice. But it seems that there is no way for annular lattice. Please see...

25 October 2023 8,964 4 View

Does population size affect mutation and how can a mutation become more prevalent in certain populations?

Does population size affect mutation and how can a mutation become more prevalent in certain populations?

24 October 2023 4,319 7 View

Decalcification on rodent nasal turbinate's?

Hello, I am planning to write my first project where I will be attempting to find an optimal decalcification time for rodent (rats specifically) nasal turbinate's, in Kristensen fluid (KF). I was...

20 October 2023 906 0 View

Does anyone have tips for expanding a vector from a supplier?

Does anyone have tips for expanding a vector from a supplier? It's ~5ng/uL and stored in 50% glycerol. I attempted to do a transformation as normal but I did not get any growth.

19 October 2023 1,258 1 View

How to align pdb files using python scipts via python in jupyter notbook ?

I want to cluster multiple pdb files with respect to one reference pdb. I have to use Align > all this to *Ca in Pymol. However, how could i do this via scripting in Pymol? As I have multiple...

16 October 2023 10,094 3 View

Why my skin frozen section always tear around the tissue, other area all very good?

I make the skin longitudinal frozen section. leica machine I conduct the temperature -30℃，but when I start section the temperature will be -23~-27 ℃，this temperature mistake？ dont know why only...

16 October 2023 9,633 1 View

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