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Questions related to Staining
I am replacing GFP with TDtomato for my sgRNA KI construct. First, I performed a PCR for TDtomato using a TDtomato plasmid as the template. When I ran the gel, I observed two bands (700 bp and...
18 July 2024 6,245 2 View
As part of my medical doctoral thesis, I am trying to improve the cryopreservation process in our laboratory. To do this, I would like to compare different glycol-based freezing media. These are...
17 July 2024 5,246 2 View
Hi, I'm going to use ReFinder to screen housekeeping genes, but I can't access to the Blooge Refinder or the (https://localhost/RefFinder-master/index.php) via XAMPP. Does anyone has ReFinder...
21 June 2024 471 1 View
Can you use the MTT assay of cell viability for tissue samples derived from rats e.g. liver, muscle, heart, brain, or is this method reserved for cultured cells? Thank you
30 May 2024 6,539 2 View
I am working on a project to detect genetic mutations in lung cancer using machine learning algorithms and I am new to genetics and medical sciences. Can I use RNA as input to the algorithm for...
14 May 2024 5,888 6 View
how to make alkali soil in lab
29 April 2024 9,777 6 View
Double-Staining of MPO and histones with SYTOX Green and DAPI
23 April 2024 5,599 0 View
Hello everyone, I am writing a systematic review about if X experimental group has superior effect than Y comparison group on outcome. Please note that the lower the scores of the outcome, the...
20 April 2024 1,982 1 View
I have respiratory epithelium which is already fixed in 4%PFA. Can i use these sections for transmission electron microscopy? I am looking for viruses on the apical surface.
10 April 2024 4,046 0 View
I am currently pursuing my PhD on ichthyodiversity of a river in my state. I want to do a comparison study, which biodiversity indices should I calculate for my study? Thanks in advance.
07 April 2024 7,787 0 View
The main reason is lack of well designed studies in the area of interest. Is there a standard tool for quality assessment of case reports?
30 March 2024 6,000 3 View
Hello dearest EV people! I want to plate my isolated EVs on coated glass coverslips and image them with confocal microscopy for CDs and other EV markers. I wonder if anyone tried a normal IF...
22 March 2024 3,454 1 View
As I remember according the classical enzymology there are four types of inhibition: Competitive, Non-competitive, Uncompetitive and allosteric Inhibition . There is an enzyme with Ca2+ as a...
22 March 2024 7,489 4 View
Benefits of accommodation establishments
21 March 2024 3,700 0 View
We are doing cell implants, and we are interested to see if anyone has any advice on using the cytopainter fluorescent dyes for detecting the cells 1 week post-implantation using frozen sections...
07 March 2024 1,217 1 View
Hi, I have to extract genomic DNA from plasma samples and will use the Qiamp DNA mini and blood kit (QIAGEN). At the moment the plasma samples are stored at -80° and they were obtained from...
05 March 2024 9,549 1 View
Does anyone suggest experimental condition (dark all time?) for measuring NO production in endothelial cells using DAF-2? Thank you Hamid
15 February 2024 3,058 1 View
I am currently investigating the safety profile of a drug in nude mice models, focusing on organs and extracted tumors. I have utilized H&E staining and IHC analysis in my experiments....
02 February 2024 1,310 1 View
I did Y2H experiment. negative control could not grow on QDO+X-alpha-Gal while positive control and my BD-gene+AD-gene could grow on QDO+X-alpha-Gal but did not turn blue.
29 January 2024 2,728 0 View
Hi, I am new to this platform. I am currently preparing for Western Blot experiments using Young (7-10 mo) and Aging (20+ mo) mice and the protein of interest will be tight junction proteins (ex....
29 January 2024 489 2 View
I have received a nanoparticle suspension from a manufacturer, which contained SDS 0.1% in stock solution. I doubt that it may influence the experiment, therefore i want to remove the SDS from...
25 January 2024 7,778 0 View
Hi, I am looking to carry out immunostaining of mauthner neurons in 4dpf larval zebrafish using the antibody 3A10 (DSHB). I have already immunostained fixed whole-larvae using a previously...
25 January 2024 351 0 View
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15 January 2024 9,250 4 View
I have several articles with two names Satyanarayana Murthy Pusuluri and PSN Murthy. How do I get all of them into my account
12 January 2024 7,959 1 View