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I am working with a recombinant protein that requires efficient endotoxin removal for downstream applications. I am considering using Triton X-114 phase separation or endotoxin removal columns....
27 February 2025 2,408 5 View
According to the International Code of Nomenclature for algae, fungi, and plants (ICN), a type must be a preserved physical specimen. The current rules do not recognize a DNA sequence alone...
19 February 2025 6,037 4 View
Hello everyone, I am using the Agilent DNA Bioanalyzer with a DNA chip for my analysis. However, when I run the chip, the generated graph appears unusual (attached for reference). I am trying to...
14 February 2025 5,152 2 View
Is CARBOSEP CHO782 column(HPLC) is compatible for using 5mmol sulphuric acid as mobile phase instead of water? Or any method is available to analyse acidic samples in same? 4% H2SO4 based sample...
12 February 2025 9,777 2 View
I would like to know if Chr 6 is diploid or not in MEG-01 cells. I have tried to find any information online, the best I can find is that MEG-01s are hyperdiploid; modal number = 56 to 58; the...
29 January 2025 8,437 2 View
I am trying to track down I looked at this morning and now I cannot find it again
28 January 2025 1,272 1 View
robot path
19 January 2025 9,967 1 View
What is the appropriate method for isolation or extraction of lipopolysaccharides by phenol from E. coli? The Phenol-water method is a commonly used and effective technique for extracting and...
13 January 2025 8,448 3 View
I am attempting to simulate quasi-static compression using the Abaqus/Explicit (2022) solver. The simulation runs successfully on my personal computer; however, when the same input file is...
13 January 2025 1,480 2 View
I am working on my master's thesis, and I want to study "Accurate lifetime statistics, prediction and repair decision of orbital critical equipment". In China, the gross passed tonnage of...
09 January 2025 5,340 2 View
I am looking for articles/journal about Pre-Operative Assessment of Patients Undergoing Elective TURBT or Rigid Cystoscopy + Bladder Biopsy: Is a Group & Save Clinically Required? I am...
06 January 2025 437 0 View
How to annotate the XYZ coordinates of an object to the camera on a 2D image?
29 December 2024 7,034 1 View
can i do weight by volume basis of the preparation ?
20 December 2024 2,668 5 View
Hi, From a long time I have been trying to get pure cytoplasmic and nuclear protein extracts from virus infected A549 cell lines. I have tried to check for the pure protein extracts of cytoplasm...
06 December 2024 6,138 2 View
hello is there a reason for HUVEC not adhering to the denuded amniotic membrane? I have read several protocols that are quite similar except for the chemical used to remove the epithelial cells...
15 November 2024 1,160 0 View
As the COO of a multinational operation, you must have a lot of experience working across cultures. Others may want to know how you manage cross-cultural teams, especially when it comes to...
13 November 2024 4,339 4 View
Business case implementing Compressed natural gas versus using established Diesel methodology
05 November 2024 2,748 2 View
What is the most suitable DNA extraction kit for isolating bacterial DNA from bile samples: the widely-used QIAamp DNA Stool Mini Kit from Qiagen or the more affordable Vivantis GF-1 Bacterial DNA...
30 October 2024 7,718 1 View
Hi everyone, I would like to isolate circRNA from a mix of circRNA, linear RNA and RNA lariats (resuspended in nuclease-free water). Please suggest optimal incubation time and temperature that has...
23 October 2024 2,980 3 View
Hi Has anyone tried to seed cells on the amniotic membrane I'm trying to seed HUVEC on the interstitial surface of the amnion but it is not working I was just wondering if there is a technique...
04 October 2024 8,479 0 View
I have been engaged in pet genetic testing for more than ten years, and there is two question that always surrounds me: What are the ethical and privacy issues of pet genetic testing? A pet’s...
03 October 2024 5,091 0 View
From a research perspective, what aspects should future follow-up studies focus on? This type of therapy has demonstrated significant tissue regeneration and repair capabilities in initial...
02 October 2024 5,200 0 View
Hello fellow researchers, I am currently working on isolating Ophiognomonia leptostyla from acervuli on walnut leaves but have been facing issues with conidial germination on PDA plates. After 10...
01 October 2024 7,645 3 View
I came across a zoonotic disease that is spread by the closest pet that is seen to be safest; - a 'pet CAT'. Toxoplasmosis is a highly infectious and fatal disease that can be of public health...
19 September 2024 2,225 1 View