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Questions related to Staining
I am analyzing RNA-Seq data, specifically focusing on the identification of variants within differentially regulated genes across treated and untreated samples. My primary analysis platform for...
02 January 2024 4,734 0 View
My stock solution of Rotenone (Sigma) is 10mM in which 0.004g of Rot is diluted by 1mL of DMSO. I have done cck-8 assay on SH-SY5Y (2 x 10^4) with Rot treatments. Several articles show that 1uM of...
02 January 2024 3,446 3 View
I am working with SHSY-5Y and using NEST-made tissue culture plates. I am looking for some companies that may support stronger adherence to these poorly adherent cell type.
31 December 2023 7,158 5 View
Hello folks, I am trying to install genice2 software.Even after satisfying the per-reqistics and installing completely when i try to run it the error i am getting is " cell: np.ndarray[3, 3] =...
27 December 2023 396 3 View
I am currently in need of an antibody targeting gene X, specifically focusing on a distinct splice variant where the major variance lies within the N-terminal region. Unfortunately, suppliers like...
27 December 2023 8,479 2 View
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25 December 2023 1,099 4 View
Dear researchers, I would like to ask you if anyone have a cracked version of Graphpad for Mac. Because I'm absolutely needing it, I don't have the free version anymore. Best regards Hind Sebbah
25 December 2023 5,722 0 View
Please suggest me few journals indexed in Scopus or WOS that only publish systematic literature reviews in the Marketing and tourism domain
22 December 2023 6,834 0 View
Due that I have forgotten my RG password, I can checked it in my iphone. Neither I can add another account.
21 December 2023 5,858 4 View
I've tried to use Ripa buffer containing 0.1% SDS + 10% of proteinase inhibitors combined with 3 cycles of freeze/thaw (Dry ice with isopropanol/Dry bath 70ºC), followed by 16.000rcf for 10...
18 December 2023 3,049 1 View
How can I improve its stability?
17 December 2023 7,698 1 View
Suppose we have a 1-2 pass heat exchanger. It's U =500 W/m2K. Now, lets say, we want to approximate it with 1-1 pass heat exchanger. Definitely, the value of U =500 W/m2K will become invalid for...
16 December 2023 3,114 4 View
During RNA FISH, the fluorescent probes are hybridized with target RNAs with 20-24bp base pairing. But how strong this interaction is with only several hydogen bonds? How much salt or fomamide...
15 December 2023 8,837 3 View
Hi, I want to isolate nuclear proteins from the diatom P. tricornutum. I can successfully isolate the nuclei, and can image them under the microscope. But I fail to lyse the nuclei. As they were...
15 December 2023 3,663 1 View
We have analysed fish and rapeseed oils for secondary oxidation products (AOCS, 1998) using the direct method protocol from Current Protocols in Food Analytical Chemistry (2001). We weighed off 25...
14 December 2023 8,381 2 View
I'm working on CD180 and TLR9 colocalization on CLL cells, but I am having difficulty getting a protocol for IF.
14 December 2023 7,260 0 View
We did a demo with Olympus/Evident APX 100 and EVOS M7000. Both look good. But we do not know all the things we will need in the future. Could you please share your experience with each of these...
11 December 2023 1,974 0 View
Is there a way to identify if a mutation in a promoter is increasing gene transcription? any application we could run a test for different mutations?
11 December 2023 5,246 1 View
I want to change .VCF file into .BED format, to import LiftOver.
08 December 2023 6,417 1 View
What is the difference between vector pET (26,21, a,,b,+.........? What are the changes made in the PET vector (for different pET)?
07 December 2023 5,596 1 View
Will equipotential surfaces always be equally spaced and equipotential surfaces help to distinguish regions of strong field from those of weak field?
06 December 2023 829 6 View
05 December 2023 3,672 6 View
Hello everyone, In the following paper, the authors do not mention how they charge the PDMS surface when studying the impact of their structures on the voltage and charge generated from their...
30 November 2023 1,088 1 View
Hello, I bought some Stevia rebaudiana seeds and tried to germinate them in vitro on MS medium. I sterilized them in a commercial bleach for 2 minutes and washed them 3 times in sterile deionized...
30 November 2023 1,477 4 View