Contact experts in Staining to get answers
1,168 views 3,247 posts
Questions related to Staining
I would like to add an epitope tag to my protein (internal, not C- or N-terminal) for imunocytochemical labelling. Do you have suggestions for specific epitopes / antibodies that may work well,...
31 May 2023 9,919 0 View
in antimitotic activity, cells of root tip get stained during geimsa staining but nucleus does not get stained, why?
24 May 2023 4,740 0 View
I'm doing a flow experiment, flowing single MCF7 cells through PDMS microfluidic chips (connected to a glass microscope slide). Currently, I'm using a Pluronic F127 (0.1 wt%) coating. However,...
22 May 2023 9,600 3 View
I want to isolate single cell clones of HepG2 from the pool using limiting dilution cloning (LDC) in 96-well plates. But I found that it's hard to get single cell suspension of HepG2 (at least 30%...
22 May 2023 492 5 View
Greetings, community! I am mastering visceral pain experiments. We are looking for an easy way to detect visceral pain specific behavior in mice. I have found a couple of articles in which...
19 May 2023 8,401 2 View
Dear peers, I have a technical question about monitoring tumor bone metastasis. We want to establish a tumor model of bone metastasis and monitor the metastatic procedure. I am aware that IVIS...
17 May 2023 1,641 0 View
I'm doing an IF experiment to show the GBP (guanylate binding protein) protein level (cytosolic protein). However I'm having trouble doing my experiment, the fluorescence signals are expressed in...
15 May 2023 2,172 4 View
antimicrobial test
14 May 2023 1,437 0 View
hematoxyline and eosin are routin stain
06 May 2023 185 2 View
Hi all, I'm trying to evaluate the amount of viable E. coli cells in a colture. I'm going to use the Nuclear Green LCS1 and I will use a plate reader to evaluate the fluorescence emitted by the...
05 May 2023 5,864 2 View
Hi My experiment is to look at the intracellular location of a small peptide. I have difficulties when I try to do the IF staining for the small peptide that has been uptaken by cells, and the...
04 May 2023 8,792 2 View
there are many types of carbohydrates(glycoprotein ) :neutral and acidic glycoprotein
01 May 2023 7,420 2 View
What are the technical steps to calculate the dilution factor for my ELISA samples (proteins or Growth factors in plasma) so I am sure I don't get min or max values in the readout results?
20 April 2023 649 3 View
Hello, I am measuring multiple different parameters in cells using fluorescent channels in flow. I would also like to assess well viability. Given that I take the same volume from each well,...
17 April 2023 2,620 4 View
Hi all, Would it be useful to monitor concentration of suspension cells or bacteria during cultivation? We are thinking of developing the device which measures optical density directly in...
13 April 2023 2,331 2 View
I want to culture mouse Kupffer cells and find appropriate media composition for the same.
13 April 2023 476 3 View
Hello Everyone, I had ordered Recombinant Alexa Fluor® 488 Anti-GFAP antibody from abcam to stain specifically astrocytes. How ever, this antibody is staining other cells too. Does anyone have a...
10 April 2023 3,160 1 View
Hello everyone. My query is, can we do docking analysis (ligand and receptor binding studies) for insecticidal toxin with the particular insect specie (like Spodoptera frugiperda) using...
06 April 2023 4,648 3 View
I have to do culture every day with mouse endothelial cells. But trans-well plate (with hanging insert) is very expensive. Is it possible to measure intercellular (tight junction) barrier...
05 April 2023 6,826 1 View
I am planning to stain cells with an Orange dye to measure intracellular hydrogen peroxide. Link for the dye kit is below. Protocol suggest examining cells at Ex/Em = 543 nm/545-750 nm. I want to...
03 April 2023 7,808 0 View
I used Millicell-24 Cell Culture Plate, fixed trans-well PCF for colonic epithelial monolayer. the PCF Filter with coated Matrigel including the cells. the filter is translucent and I couldn't...
03 April 2023 643 0 View
Hi everyone, I am currently working on lung fibrosis research on rat models of interstitial lung disease, I have problems with quantification of lung fibrosis on ImageJ. I used Masson trichrome...
02 April 2023 9,280 2 View
what chemicals can be used as alternative to the ones mentioned below (in rogosa sl broth): 1. Ammonium citrate 2. Tween 80 3. Manganese sulphate
31 March 2023 3,121 0 View
Hello, I currently do multiplex immunostaining on mouse small intestine swissroll sections. We apply the protocol described by Adrien et Guillot al (Deciphering the Immune Microenvironment on A...
29 March 2023 4,641 2 View