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Questions related to Staining
Hi, I am looking for BAC or YAC that encode for the chromosome 21. Does anyone know where I could be able to find that? Have been looking around online but it seas as it is not available to...
13 June 2023 5,383 2 View
Planning to do IF stainning of paraffin embedded urothelial tissues from Göttingen minipigs. Any insights from previous experience is warmly welcomed!
12 June 2023 2,745 2 View
I am doing the PC12-C6 co-culture system and would like to double stain the cells to observe the cell to cell interactions. I first used the mouse FITC-conjugated anti-beta III tubulin antibody,...
12 June 2023 6,689 1 View
Is there a model that I can use to estimate the long term effect of irrigation water salinity on the soil after several seasons of irrigation, which puts in consideration the crop pattern planted...
12 June 2023 6,582 1 View
I did antifungal disc diffusion test on Candida albicans and even I have repeated this test many time. However, the control positive (fluconazole) gave negative result. I used newly bought...
09 June 2023 4,047 3 View
I am looking for an analytical data to evaluate walnut rancidity, there is some knowledge on such subject ?
08 June 2023 9,812 1 View
Hello everyone, I am trying to access TNFalpha cytotoxic effect on A549 cell line ( from 2.5-40ng/ml) using Invitrogen Live/Dead staining protocol (Calcein AM/ Ethidium homodimer-1). I found that...
07 June 2023 917 0 View
Weed control efficiency calculation
07 June 2023 5,505 3 View
Hello! I need help making a 25 mM HEPES buffer with a pH of 7.4. I've tried calculating the dilution, but I keep messing up. The stock solution I am planning to use is: 30 mM HEPES Buffered...
05 June 2023 7,811 1 View
We have successfully achieved the three required temperatures on the device but no amplification has been achieved till now. Kindly suggest a method/ troubleshoot for this.
05 June 2023 9,892 0 View
I urgently need a personal copy of CLSI M100 33rd Edition of Performance Standards for Antimicrobial Susceptibility Testing
04 June 2023 271 0 View
Hi, I encountered an issue while detaching some cells. I had both HEK 293 and RAW 264.7 cells. When I added 0.05% trypsin, the cells did not round up and instead detached in the form of a sheet....
02 June 2023 1,312 3 View
I am looking for all the possible solution could be implemented to remove sticky PDMS from master mould and with it any necessary precaution needed for avoiding similar situation as shown in the...
01 June 2023 4,733 3 View
I'm trying to do a long-range PCR (~10kb) and I'm just wondering what polymerase to use despite the Q5. Do you have any experience in doing a long-range PCR with other polymerases? Did you use the...
31 May 2023 9,749 3 View
I would like to add an epitope tag to my protein (internal, not C- or N-terminal) for imunocytochemical labelling. Do you have suggestions for specific epitopes / antibodies that may work well,...
31 May 2023 9,954 0 View
Can I become a doctor for special diseases? What kind of field is the biomedical field?
26 May 2023 1,257 2 View
I found that lemongrass has a carbohydrate, but i can't find what type of sugar in it.
25 May 2023 9,057 2 View
in antimitotic activity, cells of root tip get stained during geimsa staining but nucleus does not get stained, why?
24 May 2023 4,785 0 View
I am trying to make rubber films from Natural rubber. Unfortunately, I don't have access to extruders or similar equipment and therefore work with solvents. As for my curing system, I am using...
23 May 2023 6,290 5 View
I'm doing a flow experiment, flowing single MCF7 cells through PDMS microfluidic chips (connected to a glass microscope slide). Currently, I'm using a Pluronic F127 (0.1 wt%) coating. However,...
22 May 2023 9,637 3 View
I want to isolate single cell clones of HepG2 from the pool using limiting dilution cloning (LDC) in 96-well plates. But I found that it's hard to get single cell suspension of HepG2 (at least 30%...
22 May 2023 529 5 View
Hi All, Recently, I've used BIOP JACoP plugin to analyse the protein protein colocalization during mitosis (mitotic cells, treated with Eg5 inhibitor). I struggle to understand the output of the...
22 May 2023 3,300 0 View
I want to the possibility of integrating the cosmovision underpinnings in ethnomedicine.
20 May 2023 8,233 0 View
Greetings, community! I am mastering visceral pain experiments. We are looking for an easy way to detect visceral pain specific behavior in mice. I have found a couple of articles in which...
19 May 2023 8,448 2 View