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Questions related to Staining
I want to encapsulate hydrophilic compound in PLGA and found DESD method to be best. The problem I am facing is that PLGA is not completely dissolving in Ethyl acetate. How can I fix it. I am...
23 September 2023 6,650 0 View
I am culturing primary human diabetic dermal fibroblasts using growth media during the culturing process I have encountered contamination so started to use antibiotic antimycotic. Now as anti anti...
23 September 2023 9,046 2 View
(Kindly provide reference of a peer reviewed SCI indexed journal)
23 September 2023 4,341 0 View
How do I add supplemental material from a published OA paper ?
21 September 2023 6,450 2 View
I would like to measure the area and diameter of thyroid follicles on a photomicrograph using image J (see attached image). Does anybody have a simple method for doing this given the large number...
21 September 2023 10,031 1 View
can I add a title for an "in press" accepted article before publication ?
21 September 2023 3,949 1 View
I recently did docking using PyRx program, and noticed that docking multiple ligands simultaneously gives slightly different binding energy for some of the ligands compared to when each of the...
19 September 2023 8,287 2 View
Can i add an article using API?
17 September 2023 2,027 0 View
No one knows the origin of the genetic code for DNA/RNA observed in all living organisms on Earth. Current scientific thinking is that the genetic code evolved naturally during the abiogenic...
15 September 2023 2,415 4 View
Hi everyone. I am currently working on my Undergraduate Study which is detecting Waterborne Protozoans from Water Samples. I cannot process my water samples immediately. And the travel hour from...
14 September 2023 5,761 0 View
Hi, I am getting started with confocal image analysis in Qupath. I need to know if there are any good tutorial or seminar available online for researchers like ne to get started with. I have been...
14 September 2023 6,377 0 View
If we want to culture blood cells of some wild species for karyotyping. Does we culture it after 48 hours or if not then what are the other possibilities for doing this.
11 September 2023 337 2 View
Beads-enrichment CD14 monocyte freeze by CS10 in 5E6/ml. 37℃ water bath recover in day0，viability can reach 90% above. seed in X-VIVO 15+5%PLT+GM-CSF+IL4. 1E6/ml. Day1 cell counted, viability...
11 September 2023 9,849 0 View
After searching, reading posts, and trying to solve this issue myself for several days, I think it's time to ask for help. I'm performing a Hierarchical Clustering analysis on a dataset, that...
08 September 2023 4,090 5 View
Our prodrug is activated through ROS-mediated reactions, particularly with H2O2, as indicated by our in vitro data. I'm currently working on designing an experiment to effectively reduce...
07 September 2023 7,693 0 View
I am trying to prepare samples for ATAC sequencing and the requirements. of the company is 15 nM in 20 ul volume. I ran the qubit to measure the concentration of the tagmented DNA and it was for...
05 September 2023 4,444 1 View
I want to extract recombinant OMP Wza & OMP Pal from E.coli. What are the rapid & common methods used with the using of Ultracentifuge? Need help ASAP, thank you!
03 September 2023 1,790 1 View
The image pixel addition could be performed by a sliding window over an unknown image, comparing many places on a single known image to a single known image, to find highest total pixel sums....
03 September 2023 601 1 View
Hello everybody! Could somebody explain me more about the effect of detergent in the wash buffer of a co-IP?
27 August 2023 4,371 2 View
In powdered food material, the bulk density is measured by taken the food material in a cylinder and weight / (volume occupied by material in cylinder) while for soil it is measured by core soil...
26 August 2023 2,796 2 View
Hello! We're trying to study expression of SNCA forms relative to ACTB. In example, total expression of SNCA98+112+126+140 is 50, expression of SNCA98+112 is 100, expression of 126+140 is 30. How...
24 August 2023 8,237 0 View
Nowadays, non-native English-speaking teachers (NNESTs) do outnumber native English-speaking teachers (NESTs), but the latter are still the most preferred type of teachers by the majority of...
21 August 2023 7,862 1 View
Research Gate won't let me send messages to people unless we are mutually following one another. I want to thank someone for info and cannot respond to his message. Help
14 August 2023 9,807 1 View
Hi, We have ISH kit, for which we are looking for Dig-labeled RNA positive control. I've searched and as it seems like there was a Roche product, but it has been discontinued recently. Are there...
13 August 2023 9,292 0 View