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Questions related to Staining
I need to quantify the c-fos expression in different laminae of spinal dorsal horn, can we just draw the outline acoording to the map?Or are there any morphological charateristics that can help?
03 September 2022 3,250 1 View
I want to detect markers of degranulation of T cells in HIV patients (mainly perforin and granzyme B), but I'm not sure if I need to stimulate the cells or not before test. Both are available in...
24 August 2022 5,495 2 View
Hello, I'm trying to determine the amount of glucose consumption using 14C-labelled 2-DG. What would be the good controls to use in the experiment? For negative I planned to use low glucose...
24 August 2022 5,188 2 View
How much polybrene should be added when infecting Caco-2 cells with lentivirus?
23 August 2022 3,224 1 View
Hi all, I carried out a flow cytometry in adipose tissue with a new permeabilization and fixation method in order to improve the intracellular staining for TFs... However, comparing with the...
17 August 2022 894 0 View
https://www.nature.com/articles/217168a0
15 August 2022 6,562 0 View
Hematoxylin is used as a nuclear counterstain. There are various formulations available (types of hematoxylin), classified by the mordant used.
14 August 2022 8,682 2 View
Hello all, This question is for those who use mice as a model for skin medication. One of my projects is looking to see the effects of a small molecule on the skin. I am looking for a paper that...
02 August 2022 5,209 0 View
Hi! I am counting fluoroJade positive cells and I have found clear positive FJ cells and not so clear positive FJ cells in the same brain slice... Would it be correct to count both types as...
18 July 2022 4,795 0 View
Hello all, I have sequenced results of TnSeq library of Agrobacterium tumefaciens grown at two different growth conditions. My overall goal is to see which genes are important/beneficial for the...
12 July 2022 923 4 View
I want to co-culture Neutrophils with leukemia cells (non-adherent) and want to separate the Neutrophils from the leukemia cells after the co-culture. If Neutrophils are purely adherent then I...
30 June 2022 1,153 2 View
Hello everyone, we are struggling to differentiate murine splenocytes population to enrich it with Th17-positive cells. With the protocol described shortly below, we are able to see splenocytes...
24 June 2022 9,558 0 View
Hi! I am new in flow cytometry and I would like to confront with someone who is more experienced than me. I am currently carrying out a project to determine intracellular levels of several...
05 June 2022 1,526 5 View
Hello, this is the third day that I grow L-929 cell line. Since yesterday, the cells aggregate and become like this big cluster. I want to know if this is normal. How to break the aggregation??...
03 June 2022 6,401 0 View
Hi all, I did DAPI staining for MCF-7 cells as a control (not treated with anything at all). They are fixed cells and stained with DAPI for 10 mins and washed with PBS three times. Please see the...
30 May 2022 7,582 3 View
Hello, I have a technical question regarding the measure of the SSC. Following some treatment, my cells are becoming bigger (I can see it with the microscope) and by FACS the FSC increase,...
25 May 2022 1,331 0 View
Hello, Can anyone recommend a specific FUCCI vector to image cell cycle in mammalian cells? We are looking for a vector/s with all four tagged proteins, and preferably lentiviral. Thanks in...
19 May 2022 7,308 0 View
Hello, I am trying to stain spermatozoa using eosin-gentian dye. I prepared Mann's eosin-methyl blue: 7.5 ml of10% of aqueous eosin + 0.15 ml Aniline blue directly from the stock + water to final...
26 April 2022 5,386 0 View
I was having edge effect issues with DAPI staining of spleen frozen sections. After reading over some of the posts on this topic I decided to switch from Fluouromount-G w/DAPI to adding it in...
20 April 2022 3,545 3 View
Hi guys, I am looking to optimize viability staining on peripheral blood mononuclear cells (PBMCs) with acridine orange and propidium iodide (AOPI). I will be plotting a titration curve of...
12 April 2022 6,179 0 View
Is there any other antibody which is capable of staining adult rat astrocytes for following FACS analysis other than the anti-ACSA1 antibody? Thanks in advance.
04 April 2022 5,319 0 View
I wish to conduct a study involving the geometric morphometric analysis of entorhinal cortex and hippocampal shape variations. As I hope to acquire MRI scans from online databases, I would just...
18 March 2022 1,683 2 View
I have previously stored fish skin swabs dry at -20, I was wondering if there is an alternative storage method for better DNA quality and yield.
07 March 2022 3,529 0 View
Hello. I have a question while western blot. I think I have problem with my protein extraction. First 4 lane on this picture is mine and last lane is other lab member's. Only mine looks smear. I...
03 February 2022 4,947 2 View