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Questions related to Staining
I am using pPICZalfa vector with gene length 1.1 Kb. Currently I am trying for In-vitro multicopy generation (Bgl II - BamHI), but the transformation efficiency in DH5alfa is decreasing as my...
11 January 2024 8,458 2 View
Dear colleagues, Has anyone of you ever experienced difficulties with the high background of the solvent-PBS (used to suspend the exosomes after isolation by ultracentrifugation) during NTA...
10 January 2024 7,858 3 View
Hello, We are looking to measure mean exosome size (range 50 to 150 nm) from Nanosight ns300 nanoparticle tracking data. The output is an Excel file with the average particle concentration at each...
09 January 2024 1,446 2 View
Hello everyone, I am currently engaged in a project that involves simulating core-shell structured ferroelectric nanoparticles using COMSOL Multiphysics. The specific challenge I'm facing is in...
07 January 2024 6,752 3 View
I am analyzing RNA-Seq data, specifically focusing on the identification of variants within differentially regulated genes across treated and untreated samples. My primary analysis platform for...
02 January 2024 4,872 0 View
My stock solution of Rotenone (Sigma) is 10mM in which 0.004g of Rot is diluted by 1mL of DMSO. I have done cck-8 assay on SH-SY5Y (2 x 10^4) with Rot treatments. Several articles show that 1uM of...
02 January 2024 3,586 3 View
I am working with SHSY-5Y and using NEST-made tissue culture plates. I am looking for some companies that may support stronger adherence to these poorly adherent cell type.
31 December 2023 7,249 5 View
Hello folks, I am trying to install genice2 software.Even after satisfying the per-reqistics and installing completely when i try to run it the error i am getting is " cell: np.ndarray[3, 3] =...
27 December 2023 493 3 View
I am currently in need of an antibody targeting gene X, specifically focusing on a distinct splice variant where the major variance lies within the N-terminal region. Unfortunately, suppliers like...
27 December 2023 8,592 2 View
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25 December 2023 1,253 4 View
Dear researchers, I would like to ask you if anyone have a cracked version of Graphpad for Mac. Because I'm absolutely needing it, I don't have the free version anymore. Best regards Hind Sebbah
25 December 2023 5,836 0 View
Please suggest me few journals indexed in Scopus or WOS that only publish systematic literature reviews in the Marketing and tourism domain
22 December 2023 6,950 0 View
Due that I have forgotten my RG password, I can checked it in my iphone. Neither I can add another account.
21 December 2023 5,933 4 View
I've tried to use Ripa buffer containing 0.1% SDS + 10% of proteinase inhibitors combined with 3 cycles of freeze/thaw (Dry ice with isopropanol/Dry bath 70ºC), followed by 16.000rcf for 10...
18 December 2023 3,169 1 View
How can I improve its stability?
17 December 2023 7,813 1 View
Suppose we have a 1-2 pass heat exchanger. It's U =500 W/m2K. Now, lets say, we want to approximate it with 1-1 pass heat exchanger. Definitely, the value of U =500 W/m2K will become invalid for...
16 December 2023 3,225 4 View
I need more information about this technique, and I have found only a few in some articles and about species of ants. thanks in advance.
16 December 2023 5,798 0 View
During RNA FISH, the fluorescent probes are hybridized with target RNAs with 20-24bp base pairing. But how strong this interaction is with only several hydogen bonds? How much salt or fomamide...
15 December 2023 8,941 3 View
Hi, I want to isolate nuclear proteins from the diatom P. tricornutum. I can successfully isolate the nuclei, and can image them under the microscope. But I fail to lyse the nuclei. As they were...
15 December 2023 3,797 1 View
We have analysed fish and rapeseed oils for secondary oxidation products (AOCS, 1998) using the direct method protocol from Current Protocols in Food Analytical Chemistry (2001). We weighed off 25...
14 December 2023 8,538 2 View
I'm working on CD180 and TLR9 colocalization on CLL cells, but I am having difficulty getting a protocol for IF.
14 December 2023 7,346 0 View
We did a demo with Olympus/Evident APX 100 and EVOS M7000. Both look good. But we do not know all the things we will need in the future. Could you please share your experience with each of these...
11 December 2023 2,085 0 View
Is there a way to identify if a mutation in a promoter is increasing gene transcription? any application we could run a test for different mutations?
11 December 2023 5,322 1 View
I want to change .VCF file into .BED format, to import LiftOver.
08 December 2023 6,494 1 View