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Questions related to Staining
No one knows the origin of the genetic code for DNA/RNA observed in all living organisms on Earth. Current scientific thinking is that the genetic code evolved naturally during the abiogenic...
15 September 2023 2,267 4 View
Hi everyone. I am currently working on my Undergraduate Study which is detecting Waterborne Protozoans from Water Samples. I cannot process my water samples immediately. And the travel hour from...
14 September 2023 5,686 0 View
Hi, I am getting started with confocal image analysis in Qupath. I need to know if there are any good tutorial or seminar available online for researchers like ne to get started with. I have been...
14 September 2023 6,223 0 View
If we want to culture blood cells of some wild species for karyotyping. Does we culture it after 48 hours or if not then what are the other possibilities for doing this.
11 September 2023 204 2 View
Beads-enrichment CD14 monocyte freeze by CS10 in 5E6/ml. 37℃ water bath recover in day0，viability can reach 90% above. seed in X-VIVO 15+5%PLT+GM-CSF+IL4. 1E6/ml. Day1 cell counted, viability...
11 September 2023 9,786 0 View
After searching, reading posts, and trying to solve this issue myself for several days, I think it's time to ask for help. I'm performing a Hierarchical Clustering analysis on a dataset, that...
08 September 2023 3,980 5 View
Our prodrug is activated through ROS-mediated reactions, particularly with H2O2, as indicated by our in vitro data. I'm currently working on designing an experiment to effectively reduce...
07 September 2023 7,628 0 View
I am trying to prepare samples for ATAC sequencing and the requirements. of the company is 15 nM in 20 ul volume. I ran the qubit to measure the concentration of the tagmented DNA and it was for...
05 September 2023 4,293 1 View
I want to extract recombinant OMP Wza & OMP Pal from E.coli. What are the rapid & common methods used with the using of Ultracentifuge? Need help ASAP, thank you!
03 September 2023 1,686 1 View
The image pixel addition could be performed by a sliding window over an unknown image, comparing many places on a single known image to a single known image, to find highest total pixel sums....
03 September 2023 540 1 View
Hello everybody! Could somebody explain me more about the effect of detergent in the wash buffer of a co-IP?
27 August 2023 4,237 2 View
In powdered food material, the bulk density is measured by taken the food material in a cylinder and weight / (volume occupied by material in cylinder) while for soil it is measured by core soil...
26 August 2023 2,639 2 View
Hello! We're trying to study expression of SNCA forms relative to ACTB. In example, total expression of SNCA98+112+126+140 is 50, expression of SNCA98+112 is 100, expression of 126+140 is 30. How...
24 August 2023 8,129 0 View
Nowadays, non-native English-speaking teachers (NNESTs) do outnumber native English-speaking teachers (NESTs), but the latter are still the most preferred type of teachers by the majority of...
21 August 2023 7,736 1 View
Research Gate won't let me send messages to people unless we are mutually following one another. I want to thank someone for info and cannot respond to his message. Help
14 August 2023 9,696 1 View
Hi, We have ISH kit, for which we are looking for Dig-labeled RNA positive control. I've searched and as it seems like there was a Roche product, but it has been discontinued recently. Are there...
13 August 2023 9,211 0 View
I am planning to stain a cell surface protein by immunofluorescence and then take readouts by plate reader. As I would like to detect cell surface protein, I guess cells should not be fixed. I...
08 August 2023 9,664 5 View
Hi, I was conducting few physochemical analysis for my target protein and found out that the protein located in the transmembrane region. Usually, transmembrane protein are hydrophobic and will...
04 August 2023 4,253 2 View
The color of the mold colony morphology is teal (above) and white (below). Mold isolated from soil planted with soybeans. From the microscopic images it can be seen that this mold appears to be a...
03 August 2023 4,926 0 View
We've been looking at counting amyloid plaques in mouse hippocampus using 20x fluorescence images (antibody D5452). However when using either ImageJ or Matlab code, the fill holes or watershed...
01 August 2023 5,643 3 View
any scoring system available or a quantitively method?
30 July 2023 3,907 4 View
There are many different software tools available to visualize and sometimes to edit protein structure files, eg. in PDB format. I am working on a protein with close to 1900 amino acids. I have...
28 July 2023 6,242 3 View
What are the different types of ecological pyramids and why are energy and biomass pyramids shaped like a pyramid?
28 July 2023 415 0 View
In the detection of programmed cell death, the literature shows by using Promega PCD kit, the TUNEL staining shows green fluorescence and DAPI staining shows blue fluorescence. I want to make it...
24 July 2023 7,861 9 View