Staining

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Questions related to Staining

What is the difference between vector pET (26,21, a,,b,+.........?

What is the difference between vector pET (26,21, a,,b,+.........? What are the changes made in the PET vector (for different pET)?

07 December 2023 5,667 1 View

Will equipotential surfaces always be equally spaced and equipotential surfaces help to distinguish regions of strong field from those of weak field?

06 December 2023 879 6 View

What are the 3 additional types into which AI is classified?

05 December 2023 3,747 6 View

How to charge PDMS?

Hello everyone, In the following paper, the authors do not mention how they charge the PDMS surface when studying the impact of their structures on the voltage and charge generated from their...

30 November 2023 1,169 1 View

How to germinate Stevia rebaudiana seeds?

Hello, I bought some Stevia rebaudiana seeds and tried to germinate them in vitro on MS medium. I sterilized them in a commercial bleach for 2 minutes and washed them 3 times in sterile deionized...

30 November 2023 1,530 4 View

QPCR Standard Curves showing unexpected signals?

I am currently generating a standard curve for two targets highly specific to my organism using a TaqMan probe-based qPCR assay and standard serial dilutions ranging from 10^6-10^1 gene copies. I...

28 November 2023 297 3 View

Can anyone recommended me best suited activation function in neural networks for real time scenarios?

Iam planning to do some literature work on rationale neural networks and functionalities of activation functions like sigmoid and others, please recommend me some effective articles related one

26 November 2023 1,209 3 View

How to draw a protein secondary structure topology from protein sequence?

Dear all, Can anyone provide me with a software or a webtool for drawing 2D-topology for a protein from a sequence similar to the one provided in the following figure?

17 November 2023 8,466 2 View

Why there is cytoplasmic stain of KI67 in HCC mouse liver tissue?

When stained with KI67 in mouse liver tissue with HCC, I can see cytoplasm stained and not nuclear staining. This is IF staining where the tissue is fixed with formalin and permeabilized with 0.2%...

15 November 2023 266 1 View

Importing model with CIN3D8 infinite Elements , removes existing sets! How to create node sets again with the resulting Orphan mesh?

I have created an Abaqus model using a Python script, the script creates the model, and defines the material, the assembly, the partitions, and the creation of sets of points, since I want to use...

15 November 2023 1,210 2 View

How to get the permission to use MARS-5 (Medication Adherence Report Scale-5) questionnaire for my research?

Hello everyone. I am planning to use the MARS-5 (Five item Medication Adherence Report Scale) questionnaire for my research. I want to know how should I get the permission to use this scale. Your...

13 November 2023 9,181 3 View

Is anyone currently using a programmable peristaltic pump with 4-roller pumping heads and 1/16" ID tubing from Braintree Scientific, Inc.?

I am in the process of setting up a programmable peristaltic pump for mouse cardiac perfusion. I'm uncertain about the appropriate blunt needle specifications, specifically 24 gauge (24g) with a...

13 November 2023 9,729 0 View

I'm trying to calculate a Soil Quality Index using the weighted additive SQI method. Do you have any R code that you can provide?

09 November 2023 543 2 View

How to isolate Golgi Vesicles from cultured cells?

I want to isolate Golgi vesicles from Hela cells by flotation through a discontinuous sucrose gradient. Theoretically, Golgi band will be harvested from the 0.8 M/1.2 M sucrose interface. But I...

03 November 2023 2,497 0 View

Hi, anyone working on cas12 protein for nucleic acid detection? I also wish to know how to design crRNA?

I was looking for nucleic acid detection with CRISPR. I wish to know how and where to design the crRNA? Which company deals with it? Any lead would be appreciated.

02 November 2023 560 1 View

Best way to preserve FACS enriched cells for downstream single nucleus RNA & ATAC seq?

I am working with a cell population that needs to be enriched by cell sorting from fresh tissue. I am wondering what the best way to preserve the cells, after sorting, for downstream single...

31 October 2023 9,234 0 View

How can deep learning models be optimized to improve the early detection of various cancer types using medical imaging data?

31 October 2023 2,598 5 View

What strategies can be employed to overcome the limited availability of labeled data for training deep learning models in cancer identification?

31 October 2023 4,079 3 View

What is the protocol to sonicate surgical instruments?

Hi, I'm exploring ways to sterilise my instruments used in cell culture to dissecte unfixed live human eye tissue. Does anyone have an ideal protocol for sonication or other sterilisation...

30 October 2023 4,685 2 View

Are there any ongoing collaborations within the realm of fixed point theory?

I am currently researching various contractions using a measure of noncompactness for both single and multivalued mappings. My study focuses on exploring the existence of solutions for equations...

30 October 2023 4,997 3 View

What is the purpose of Sephadex G-100 separation after PEG precipitation for exosomes/EVs in a publication?

Hello! I am reviewing a procedure for exosome/EV purification from a large-volume blood sample (porcine, terminal draw). In brief, protocol is: 1) Blood collection 2) Two-step spin to get...

27 October 2023 6,938 1 View

Nanoparticles impact on animal tissues: Histological (H&E) analysis of liver tissues, revealed the presence of slightly orange-colored cells?

Nanoparticles impact on animal tissues: Histological (H&E) analysis of liver tissues, revealed the presence of slightly orange-colored cells....... Does anybody observe similar structures in...

25 October 2023 5,320 3 View

Alcian blue staining protocol for glutaraldehyde-fixed chondrocytes?

I have fixed human chondrocytes in 6-well plate by glutaraldehyde. For alcian blue staining pH 1, I used this protocol: 1) Rinse wells 3x with PBS. 2) Rinse 1x with 1% acetic acid. 3) Apply...

25 October 2023 7,457 0 View

Annular lattice in MCNP?

Dear all. I need to describe an annular lattice in MCNP. I found the way to create a hexagonal lattice or rectangular lattice. But it seems that there is no way for annular lattice. Please see...

25 October 2023 9,030 4 View

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