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Questions related to Staining
Hi, I would like to inject tamoxifen to mice pups from P7 to P11 once daily. I’ve tried injecting 30ul of tamoxifen with 27G needle to intraperitoneal space but most leaked out. I came across an...
24 January 2023 7,768 1 View
Is it the sum or average of all SDs?
18 January 2023 7,656 4 View
I am inducing ischemia by OGD in vitro. If I want to estimate mitochondrial membrane potential or look at ROS levels by means of fluorescent dyes, can I fix the cells and then stain for these? I...
18 January 2023 2,222 4 View
I am using β-actin as a loading control for my western blots to enable quantification of proteins. I never used to have an issue with this protein but recently I am getting faint bands during...
17 January 2023 1,838 4 View
I recently performed an experiment where I coated a CD3 antibody on a 96 well (flat-bottom, TC treated) plate at a concentration of 4 µg/ml (50 µl per well) for 2h at 37°C, 5% CO2. Afterwards, I...
17 January 2023 1,875 2 View
Hi, I would like to use Apotracker green (Biolegend) with other surface antibodies. Does someone have experience with it ? Would you add the Apotracker Green together with the other antibody or...
14 January 2023 9,078 3 View
Its been some time since i worked with enzymes. So as i now had a look at Enzymes by several vendors i wondered about the units the activity was given in and their meaning. e.g browsing Novozymes...
13 January 2023 8,249 2 View
for how many times can I use/re-use Giemsa stain to stain my slides? and would that affect the tone of the stain and make it lighter?
12 January 2023 7,355 2 View
I have a question about a picture I took with the confocal microscope. I isolated PBMCs from human blood using ficoll. I centrifuge it 15 minutes at 800xg without a brake. After isolating the...
11 January 2023 7,286 3 View
Did anybody ever attempt to combine a X-gal staining with an antibody staining ? We would like to try but if somebody else has done this and it works or not that would be very helpful.
11 January 2023 8,689 0 View
I want to stain a microorganism with a sterol specific fluorescent-dye for FACS and reculture high sterol producers after sorting. I know the fluorescent probe Filipin is available for staining...
10 January 2023 4,531 1 View
I am preparing Rifampicin (injectable) with a final required concentration of 5μg/5μL by dissolving a 450mg tablet in 100% DMSO. I want to inject 5μL of the drug solution into my animal model. The...
08 January 2023 4,413 1 View
TNF alpha, IL-6, and IL-1b protein expression has not elevated after the colon and small intestine tissues(.5cm ) were treated with one microgram/ml for tissue incubation with DMEM media for 6...
08 January 2023 4,533 4 View
Somehow this question ended up in the "Penicillin" category? So trying again. I am working with primary murine jejunal enteroids/spheroids, but I am having difficulty keeping them growing...
29 December 2022 6,790 0 View
Hi, We're experiencing troubles with THP-1 differentiated to macrophages immunostaining. The nucleus is not uniformly stained with Hoescht, not even the protein we want to stain. Any suggestion on...
20 December 2022 4,158 0 View
can anyone can suggest a polymer thickening agent, possibly aerosol ?. We are using nanocomposite polymer solution for dip coating. Polymer is too runny. Thx
16 December 2022 7,931 5 View
Hi everyone. I am working with immunofluorescence, and for three different targets, which should all show up in the nucleus I am having the same issue of what looks like positive staining with...
16 December 2022 462 10 View
We use the LIVE/DEAD™ BacLight™ from Invitrogen™ (L7012, ThermoFisher Scientific) to look at the effect of drug compounds against Pseudomonas aeruginosa biofilms, in in vitro static conditions....
11 December 2022 6,527 1 View
Are neural crest cells the same as neural crest stem cells? what are their specific markers?
07 December 2022 7,307 1 View
hello good day for everyone if anyone can help me what is the most suitable fixative for striations in cardiac and skeletal muscular tissue to fix striations and sarcomere sand what is the...
24 November 2022 9,372 2 View
Hello, I would like to know whether the following compounds can be used interchangeably for Van Gieson staining: Acid fuchsin: https://pubchem.ncbi.nlm.nih.gov/compound/Acid-fuchsin Acid fuchsin...
24 November 2022 250 1 View
I was trying to prepare a calcein solution with the quenching concentration of the calcein dye. I could not completely dissolve the calcein powder either in a pH nine buffer or in a minimum volume...
23 November 2022 8,088 0 View
So the way I've been doing it is I suspend the cells in 5mL DPBS, take a small sample and count it, then centrifuge, aspirate off supernatant, and resuspend pellet in 1:1 cell PBS and 10%...
21 November 2022 2,595 0 View
Hi, I am a PhD student who is trying to use synthetic amyloid oligomers. However, I have found that there are so many different types of techniques and methods. I would be grateful if you anyone...
21 November 2022 5,959 0 View