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Questions related to Staining
Hi. I need help to ID this white colony (4-7 days old at room temp). If more than 7 days of incubation, it turns green on top of the PDA agar and yellow at the bottom.
19 May 2023 1,542 1 View
Hi, I am trying to use MATLAB gamultiobj, but I need the variables to be between -1.5 and 1.5, but after it runs I realize it didn't take them into account. The variables exeed the conditions. I...
18 May 2023 7,858 2 View
Dear peers, I have a technical question about monitoring tumor bone metastasis. We want to establish a tumor model of bone metastasis and monitor the metastatic procedure. I am aware that IVIS...
17 May 2023 1,706 0 View
I'm doing an IF experiment to show the GBP (guanylate binding protein) protein level (cytosolic protein). However I'm having trouble doing my experiment, the fluorescence signals are expressed in...
15 May 2023 2,218 4 View
Hello everyone, lately I'm working with spheroids of primary mice intestinal stem cells. I'm trying to set up some 2D culture as well, trypsinizing the spheroids and seeding the individualized...
15 May 2023 8,337 3 View
antimicrobial test
14 May 2023 1,487 0 View
Unexplored traditional knowledge systems and techniques in Indian handicrafts and art forms encompass a wide range of practices. These include: Textile Weaving: Unexplored techniques in textile...
12 May 2023 827 0 View
hematoxyline and eosin are routin stain
06 May 2023 237 2 View
Hi all, I'm trying to evaluate the amount of viable E. coli cells in a colture. I'm going to use the Nuclear Green LCS1 and I will use a plate reader to evaluate the fluorescence emitted by the...
05 May 2023 5,916 2 View
Hi My experiment is to look at the intracellular location of a small peptide. I have difficulties when I try to do the IF staining for the small peptide that has been uptaken by cells, and the...
04 May 2023 8,832 2 View
there are many types of carbohydrates(glycoprotein ) :neutral and acidic glycoprotein
01 May 2023 7,472 2 View
My research direction is germination of actinomycete spores,and I can't tell if the spores have germinated under a light microscope at 100X.
30 April 2023 5,498 2 View
While collecting primary data with the help of a structured questionnaire, which is the best way to collect information on income/saving/expenditure? For ex: 1. Amount spent on the purchase of...
27 April 2023 7,365 2 View
I am preparing to do intracerebroventricular injection in mice, what is the volume of cerebrospinal fluid in mice?
26 April 2023 8,669 1 View
Hello, We are currently measuring concentrations of protein kinase A (PKA) and its phosphorylated from (p-PKA) in sub regions of the hippocampus using α-tubulin as loading control. We are using...
25 April 2023 6,434 0 View
Could anyone please provide me with the information on digital pathology slide scanner? Having computer programming software.
24 April 2023 8,960 1 View
Hi, Looking for sample tube formation assays picture for image J vessel analysis practice. Also, What is your take on Image J for measuring vessel interbranch distance and branching angles? are...
23 April 2023 7,699 1 View
What are the technical steps to calculate the dilution factor for my ELISA samples (proteins or Growth factors in plasma) so I am sure I don't get min or max values in the readout results?
20 April 2023 725 3 View
Hello, I am measuring multiple different parameters in cells using fluorescent channels in flow. I would also like to assess well viability. Given that I take the same volume from each well,...
17 April 2023 2,698 4 View
Hi all, Would it be useful to monitor concentration of suspension cells or bacteria during cultivation? We are thinking of developing the device which measures optical density directly in...
13 April 2023 2,368 2 View
I want to culture mouse Kupffer cells and find appropriate media composition for the same.
13 April 2023 505 3 View
Hello Everyone, I had ordered Recombinant Alexa Fluor® 488 Anti-GFAP antibody from abcam to stain specifically astrocytes. How ever, this antibody is staining other cells too. Does anyone have a...
10 April 2023 3,201 1 View
Hello everyone. My query is, can we do docking analysis (ligand and receptor binding studies) for insecticidal toxin with the particular insect specie (like Spodoptera frugiperda) using...
06 April 2023 4,696 3 View
I have to do culture every day with mouse endothelial cells. But trans-well plate (with hanging insert) is very expensive. Is it possible to measure intercellular (tight junction) barrier...
05 April 2023 6,873 1 View