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Questions related to Staining
What is stained blue and what is stained red exactly in bone with masson trichrome? I read different descriptions. Can I have a reference too?
17 January 2022 9,541 0 View
Is there any paper about using Tunel staining to detect mitochondrial DNA (mtDNA) damage?
10 January 2022 5,387 1 View
I used to do EdU + ClickIt staining regularly. 2 months ago it stopped working. Specifically, in FACS, all of the cells are positive every time, despite my changing the cell type and all of the...
29 December 2021 5,013 0 View
I am exploring the use of 2D gel DNA electrophoresis for analyzing plasmid DNA replication in vitro. I have found a number of articles that utilize this technique for this purpose, but they all...
29 December 2021 4,196 2 View
Hey guys, I am currently stimulating cryo pbmcs for 18 hours with lps (100ng/ml) right after thawing these cells. After my incubation I stain these cells extracellulary for CD14 and CD16 for...
15 December 2021 2,948 2 View
Hi, I am new (next to zero experience) to circular dichroism. Can anyone tell me what secondary structure is associated with the following CD spectra? After some reading, I found this article...
05 December 2021 7,825 8 View
I need to do troubleshooting for getting proper RNA bands in the gel. So any method or way of troubleshooting is welcome. And my samples are either getting stuck in wells of the gel or are getting...
26 November 2021 3,003 2 View
I have some adherent cells in a flask. Early next week, some of those cells will be trypsinized, passaged and seeded in a new flask for future experiments; so far so good. The rest of the cells...
25 November 2021 8,407 5 View
Hello, I need to stain in a complete way all the cell membrane of a zebrafish larvae around 6dpf. I approached bodipy, but this staining doesn't work in fixed larvae. Some of you have some ab,...
24 November 2021 7,923 2 View
All my Selaginella got contamination after sterilization phase. I already try to change method for sterile it such :- 1. soak them in fungicide + tween 20 2. rinse with dh2O (3times) 3. dip in...
18 November 2021 8,909 1 View
Can you stain and image (via TEM) bacteriophage directly from lysate? (10^9 PFU/ml suspended in SM-buffer) Or do phages need to be purified via other means prior to imaging?
25 October 2021 7,503 2 View
I have a sample of Trypan Blue that looks like a tangle of threads in blue and transparent background, but I am not sure if that is what the sample should look like or if it is contaminated and...
14 October 2021 4,557 1 View
My surface stain is Mouse Anti porcine CD3e AF488 for T cell and Mouse Anti porcine CD21 AF647 for B cell. and i am using Mouse anti rabbit PE for intracellular staining? Can i use Mouse seroblock...
06 October 2021 7,698 3 View
Heme is known to bind to cytochrome C with thioester bonds involving the cysteine residues. However, the paper that reports the three-dimensional structure of cytochrome C from bovine heart (PDB:...
29 September 2021 939 2 View
I'm looking for an enzyme, deglycosylation kit or chemical method for deglycosylation that preserves the protein and/or the oligosaccharides. It is an O-linked glycoside.
16 September 2021 3,009 4 View
I am doing IHC with mouse cornea and after adding Prolong Gold Anti-fade mounting media, there are now bubbles on the slides. How can I remove the bubbles? Thank you!
15 September 2021 7,632 3 View
Live cell imaging using IncucyteZoom is the best way to measure effect of drugs on rate of cell proliferation. I noticed that despite of how accurate we try to count the cells using automated...
13 September 2021 7,132 3 View
Attempting to stain the nucleus of live yeast cells for microscopy viewing after metal treatment. The Hoechst dye is a little difficult to tweak and work with as it preferentially stains nucleus...
10 September 2021 9,548 0 View
i am working on rheological properties of polymeric blends hydrogel but if i want it to correlate with structural studies of gel samples ,can we do other characterizations of hydrogel samples?
10 September 2021 9,381 3 View
Any suggested readings for plant programmed cell death techniques? (DAPI staining, DNA laddering, etc.)
02 September 2021 2,458 2 View
I synchronized the cells at G1 stage and then released them.with different cell cycle inhibitor. I found out that cells treated with G1 inhibitor has low no of cells in G1 phase comparing to other...
19 August 2021 6,796 0 View
Hi everyone, Can someone share their optimal concentrations of PMA/ionomycin for spleen T cells? I plan to use PMA/ionomycin as a positive control for T cell activation (surface markers,...
18 August 2021 7,366 3 View
Could anyone share the protocol for cell recovery from gelatin scaffolds without a substantial loss of cell number?
17 August 2021 4,720 3 View
Dear all, I realized that Biolegend has all its Treg staining kits as AF488 conjugated FoxP3 and other dyes for CD25/CD4. What is the reason behind it that they are changing CD25 and CD4...
13 August 2021 3,567 2 View