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Questions related to Staining
I am planning to stain a cell surface protein by immunofluorescence and then take readouts by plate reader. As I would like to detect cell surface protein, I guess cells should not be fixed. I...
08 August 2023 9,729 5 View
Hi, I was conducting few physochemical analysis for my target protein and found out that the protein located in the transmembrane region. Usually, transmembrane protein are hydrophobic and will...
04 August 2023 4,506 2 View
The color of the mold colony morphology is teal (above) and white (below). Mold isolated from soil planted with soybeans. From the microscopic images it can be seen that this mold appears to be a...
03 August 2023 4,986 0 View
We've been looking at counting amyloid plaques in mouse hippocampus using 20x fluorescence images (antibody D5452). However when using either ImageJ or Matlab code, the fill holes or watershed...
01 August 2023 5,709 3 View
any scoring system available or a quantitively method?
30 July 2023 3,977 4 View
There are many different software tools available to visualize and sometimes to edit protein structure files, eg. in PDB format. I am working on a protein with close to 1900 amino acids. I have...
28 July 2023 6,335 3 View
What are the different types of ecological pyramids and why are energy and biomass pyramids shaped like a pyramid?
28 July 2023 475 0 View
In the detection of programmed cell death, the literature shows by using Promega PCD kit, the TUNEL staining shows green fluorescence and DAPI staining shows blue fluorescence. I want to make it...
24 July 2023 7,931 9 View
Can anyone explain why a study's ELISpot assays might be designed wherein its cells are being plated at a concentration of 200K/well (the standard amount) and then 100K/well? The concentration of...
24 July 2023 5,561 1 View
Why is the rate of decomposition affected by pH of soil and hat environmental factors do decomposers require to increase their activity?
23 July 2023 1,949 2 View
I would like to transfect HEK293 with a plasmid containing a luciferase (downstream of a response element to this pathway) to check a cell signal pathway, but in order to have a population of...
21 July 2023 904 1 View
I want to analyze the following T regulatory cell phenotypes: CD4+CD25+IL-10+ T regs, CD4+CD25+Foxp3+ T regs, and CD4+LAP+ (TGF-beta1-secreting) T regs. The IL-10+ and LAP+ T regs seem to have to...
20 July 2023 991 0 View
Hi, I am currently using pHrodo Green AM as a marker for my target cells, which was used in a phagocytosis assay with my M1-like THP-1 cells and I have some questions regarding this specific...
18 July 2023 10,006 1 View
I am new to creating solutions, and any help would be beneficial!
18 July 2023 9,165 0 View
I am getting absorbance in 6 range nad transmittance in 6000 range even in the absence of sample. when sample is placed similar results obtained. In background mode only flat line at zero...
18 July 2023 2,392 3 View
I am working on a project identifying molds grown on hard cheese. One of my plates (PDA) had growth that looked like yeast. I have been staining the different mold samples using LCPB. I attemped...
17 July 2023 691 1 View
Assuming that both are heat inactivated and sterile filtered, is there a difference between normal goat serum and donor goat serum. I can't seem to find any major difference between Is their an...
17 July 2023 4,296 2 View
Dear researchers, I am performing heart staining with TTC. after staining, the tissue becomes hard, rough and shrinks and i am unable to take photographs. How I can avoid it?
17 July 2023 5,093 1 View
how to know about different types of contraction mapping to prove fixed point theorem like ciric, rus, f-contraction, suzuki contraction etc
16 July 2023 4,921 1 View
Is photosynthesis done by producers or consumers and bacteria that perform photosynthesis primary producers or consumers?
13 July 2023 5,778 6 View
Hi everyone. I have protein with concentration of 0.6 mg/mL The total volume of my protein is 4 mL. Protein size is ~18 kDa How can I convert my total protein concentration to micro...
11 July 2023 8,646 8 View
I will do qPCR from DNA genome. My DNA concentration is only 2-0.5 ng/uL. What is the minimum concentration of DNA template for qPCR? If need more DNA concentration, what should I do? Thank you...
07 July 2023 399 2 View
I have a problem with number of cells in E0771 cell line . I usually use 100-2000 cells for sphere but I couldn't get good sphere.
05 July 2023 1,148 6 View
I am doing westernblots. Thee independent experiments. In each experiment i get different result. For example, if i see a decrease in my target protein at 10ng/ml then in another repetition, it is...
04 July 2023 5,117 3 View