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Questions related to Staining
When Crystallographic file of the protein contains more than 1 part , in interaction with each other while they are of two different conformer of same protein , what can be the optimal approach?...
05 April 2023 7,887 3 View
Hi everyone, We want to start performing some FluoroJade C stainings at the lab but the product that other labs have recommended is longer available (AG325 from Sigma-Aldrich). Do you have any...
05 April 2023 9,608 2 View
Hi everyone, I'm using the Sartorius Incucyte SX5 for in vitro live imaging experiments and I'd like some clarification about the quantification of the fluorescent signal. The provided software...
04 April 2023 1,354 0 View
I am planning to stain cells with an Orange dye to measure intracellular hydrogen peroxide. Link for the dye kit is below. Protocol suggest examining cells at Ex/Em = 543 nm/545-750 nm. I want to...
03 April 2023 7,854 0 View
I used Millicell-24 Cell Culture Plate, fixed trans-well PCF for colonic epithelial monolayer. the PCF Filter with coated Matrigel including the cells. the filter is translucent and I couldn't...
03 April 2023 700 0 View
Hi everyone, I am currently working on lung fibrosis research on rat models of interstitial lung disease, I have problems with quantification of lung fibrosis on ImageJ. I used Masson trichrome...
02 April 2023 9,326 2 View
what chemicals can be used as alternative to the ones mentioned below (in rogosa sl broth): 1. Ammonium citrate 2. Tween 80 3. Manganese sulphate
31 March 2023 3,156 0 View
Hello, I currently do multiplex immunostaining on mouse small intestine swissroll sections. We apply the protocol described by Adrien et Guillot al (Deciphering the Immune Microenvironment on A...
29 March 2023 4,688 2 View
Hey, I´m struggling with the results of the two methods. I use the Bioanalyzer (RNA 6000 Pico Kit) and Nanodrop to measure the concentration and integrity of my mRNA samples. I heat denature the...
29 March 2023 687 1 View
We are trying to prepare healthy striatal brain slices to do some whole cell patch-clamp electrophys recordings. The brain slices that we get seem to have (selectively?) lost the MSNs - they look...
29 March 2023 4,126 3 View
I conducted an extensive search for a comprehensive database that encompasses the biological processes involved in various diseases. Although there are existing resources, such as KEGG and...
28 March 2023 6,297 1 View
I wish to download TCGA gene expression from XENA browser using the following...
28 March 2023 6,098 2 View
I am doing ovarian histology and fixing the head, tail trimmed carcass in Davidson's fixative. My sample oocytes get crumble when I section.
24 March 2023 5,175 0 View
while doing klenow reaction for blunt end cloning,i am not getting why it is necessary to add dNTPS to chew 3' overhangs
24 March 2023 3,812 1 View
I'm trying to do CHIP-qPCR on various cells lines on both histone markers and TF. The problem I've encountered is both high background in non-binding intergenic regions and also high binding in...
23 March 2023 9,359 0 View
we made a whole genom sequence of some bacterial isolates and we need a pefect tool for analysis of fasta sequence
21 March 2023 4,204 2 View
I am trying to calculate volume of an unknown protein sample from bradford assay. Help me in estimating protein volume for SDS PAGE gel .
21 March 2023 2,910 3 View
I'm currently searching for comet assay kit, which can give almost accurate results and should be cheap of cost. Can anyone recommend any kits regarding this that you have worked with?
20 March 2023 1,437 2 View
In our lab we started working with precision cut liver slices of HCC and CCC. We use William's Medium E with Phenol Red as a pH indicator. We observed a rapid color change from pink to yellow...
20 March 2023 9,582 0 View
This author has accidentally claimed paper from Ferid Murad for himself. Can you please correct this?
19 March 2023 5,237 2 View
I am new to extracting RNA from fruit flesh. Currently, I'm extracting RNA from sweet cherry flesh using Fruitmate and Nucleospin Takara Bio. I have tried using RNeasy kit Qiagen but I failed (but...
17 March 2023 4,833 2 View
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16 March 2023 1,666 3 View
I am trying to use the Ovation RNA seq V2 kit from Nugen or Tecan to amplify low-input RNA (about 1ng). (https://lifesciences.tecan.com/ovation-low-input-rna-seq-kit-v2?p=tab--1). The Protocol...
14 March 2023 2,093 2 View
1. Is TMEM192 the only selectable protein in LysoIP? 2. If I'm going to do LysoIP, should I make sure the TMEM192 protein expression do not change first? 3. After LysoIP, if I want to confirm...
14 March 2023 1,424 1 View