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we have employed the metachromatic stain acridine orange for visualization of nucleic acids in gels. This dye interacts differently with double- and single-stranded polynucleotides, fluorescing...
08 August 2021 3,402 0 View
Hi guys, I've suspected my cell culture for contamination so I tested simple DAPI staining by cell fixation using ice cold methanol for 15 min followed by DAPI staining for 15 min. The result...
07 August 2021 9,683 5 View
Hello all, I am currently trying to culture cortical neurons in media free of T3 in hopes to observe change when supplementing the cells with different concentrations of T3. I have looked up some...
05 August 2021 4,422 2 View
Hi all, I am trying to stain the inner cell mass (ICM) of fixed human embryos with TUNEL to detect apoptosis (In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich) for confocal...
04 August 2021 3,653 2 View
Hi, I'm working with CRISPR Knock-in experiments, and I need a tool to check the insertion I have. I've been using ICE from Synthego for analysis of DNA sequencing data. However ICE is not able...
28 July 2021 4,173 5 View
Hello! I am getting some smearing appear on these 4-20% gradient gels. I loaded 20ug of protein each time. I am seeing my bands fine but the rest of the gel is a bit of a mess. I have attached an...
28 July 2021 2,552 3 View
I have fluorescent bands on the gel, the pictures for analysis quality is black (background) and white (bands). With ImageJ, if I don't do Edit-Invert before Select Lanes or Invert peaks before...
27 July 2021 749 3 View
I want to run flow cytometry on my samples to detect cell death. Samples include negative control (untreated cells will have the most healthy living cells), positive control (STS treated cells...
29 June 2021 4,041 1 View
My friend and I have been doing SSCP using silver staining for the past few days.We run it at 110V, 350mA and for 3 hours.Her product size is 250bp. We are unable however to see lanes or the...
26 June 2021 2,619 3 View
I am going to measure the leaf water content from coal char and biochar treated soils to see the soil moisture content change in soil due to char application and its reflection in plant leaf water...
22 June 2021 7,162 8 View
What is the Giemsa Stain protocol utilized to prepare and analyze root metaphase chromosomes? Thank you
14 June 2021 7,793 3 View
Hi, I'm doing my PhD with mdx animal heart and would like to evaluate with alizarin stain. I found a protocol, but the consistency of the Alizarin was very dense and I think it's not right. Does...
12 June 2021 8,787 0 View
Dear all, We have performed a classical trypan blue stainning. We would need to decolore it, but by using no toxic products. Do you know if alternatives to chloro hydrate decoloration are...
10 June 2021 7,576 2 View
I am trying to perform osteoblast quantification using H&E. I have been reading papers (like this one: Extracellular matrix with defective collagen cross-linking a... ) where measurements like...
06 May 2021 2,136 2 View
Isolation of protozoans from water samples
30 April 2021 5,014 1 View
I'm growing mold and yeast in the same liquid medium, can you tell the difference between yeast and mold spores under a microscope? I tried to differentiate it by using lactophenol as a staining...
28 April 2021 7,784 9 View
If PVN already injected with CTB, so can I stained for CRH and c-fos for the same PVN tissue injected with CTB?
24 April 2021 7,312 0 View
I would like to quantify hyaluronic acid in the skin section of WT vs Mutant mice. Is Alcian blue staining is a good pick? Any recommendations are appreciated. Thanks
22 April 2021 5,288 1 View
Hello everyone, I'm trying to stain human blood PBMC and monocytes at day 0 and day 6 M2 macrophages (obtain after differentiation from monocytes). After FACS analysis of my cells, first I don't...
22 April 2021 9,301 5 View
Hello, I am performing an immunofluorescence TUNEL assay using an abcam kit (ab66110) on frozen tissues of kidney mice using the following protocol from abcam (frozen tissues section)...
15 April 2021 436 2 View
We are working on comet assay experiment in single cell electrophoresis using bromide ethidium. We have ruled out cell absence in the samples and there does not seem to be any problem concerning...
15 April 2021 1,412 2 View
However, since Sprague Dawley rats are known to grow tumors at a high (and very variable) rate, the study was considered flawed in design and its findings unsubstantiated
14 April 2021 5,659 3 View
please advice... as I would be performing histological staining using alcian blue Haematoxylin staining on zebrafish cryosections to look at cartilage element or strs.
14 April 2021 7,697 3 View
Various fluorochromes such as CMA3 and DAPI are used in chromosome banding. Base-specific fluorochromes such as CMA3 (GC) and DAPI (AT) are also used to distinguish heterochromatic segments that...
05 April 2021 9,796 1 View