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Questions related to Staining
I tried ACN, DMSO and other solvent like methanol, water but its seems that peptide is not soluble and it is forming a gel in ACN and water. The peptide has 10-12 amino acid.
03 July 2023 7,404 1 View
Here is an image I tried to take using TEM ( JEM-1400S) for human adenovirus. I used uranyl acetate to negatively stain the sample. However, this image I have indicates the sample is positively...
28 June 2023 514 1 View
I have found a Bacillus isolate that showed antimicrobial activity against gram positive and negative ATCC test strain. I have done the test three times where showed same activity against test...
24 June 2023 9,024 2 View
How can preparation of sample for western blotting from isolated pancreatic islet be done?
24 June 2023 7,948 0 View
I'm using the R&D ELISA kit to read Lipocalin and Myeloperoxidase. The kit says to read at 450nm with correction of 540nm or 570nm but the Biotek 800TS absorbance reader only has 490nm and...
23 June 2023 6,639 0 View
After staining, the sections appear very light. Should I increase the incubation time in DAB?
19 June 2023 860 1 View
I'm using the Biotechne Human S100A8/S100A9 ELISA kit to assay fecal calprotectin but struggling to get the components needed for the recommended extraction buffer in the protocol of the kit: 0.1M...
19 June 2023 6,798 0 View
After extraction of folic acid from probiotic bacteria is there any further purification step?
16 June 2023 6,312 6 View
Hi, I am looking for BAC or YAC that encode for the chromosome 21. Does anyone know where I could be able to find that? Have been looking around online but it seas as it is not available to...
13 June 2023 5,444 2 View
Planning to do IF stainning of paraffin embedded urothelial tissues from Göttingen minipigs. Any insights from previous experience is warmly welcomed!
12 June 2023 2,815 2 View
I am doing the PC12-C6 co-culture system and would like to double stain the cells to observe the cell to cell interactions. I first used the mouse FITC-conjugated anti-beta III tubulin antibody,...
12 June 2023 6,733 1 View
Is there a model that I can use to estimate the long term effect of irrigation water salinity on the soil after several seasons of irrigation, which puts in consideration the crop pattern planted...
12 June 2023 6,643 1 View
I did antifungal disc diffusion test on Candida albicans and even I have repeated this test many time. However, the control positive (fluconazole) gave negative result. I used newly bought...
09 June 2023 4,082 3 View
I am looking for an analytical data to evaluate walnut rancidity, there is some knowledge on such subject ?
08 June 2023 9,880 1 View
Hello everyone, I am trying to access TNFalpha cytotoxic effect on A549 cell line ( from 2.5-40ng/ml) using Invitrogen Live/Dead staining protocol (Calcein AM/ Ethidium homodimer-1). I found that...
07 June 2023 953 0 View
Weed control efficiency calculation
07 June 2023 5,603 3 View
Hello! I need help making a 25 mM HEPES buffer with a pH of 7.4. I've tried calculating the dilution, but I keep messing up. The stock solution I am planning to use is: 30 mM HEPES Buffered...
05 June 2023 7,862 1 View
We have successfully achieved the three required temperatures on the device but no amplification has been achieved till now. Kindly suggest a method/ troubleshoot for this.
05 June 2023 9,961 0 View
I urgently need a personal copy of CLSI M100 33rd Edition of Performance Standards for Antimicrobial Susceptibility Testing
04 June 2023 326 0 View
Hi, I encountered an issue while detaching some cells. I had both HEK 293 and RAW 264.7 cells. When I added 0.05% trypsin, the cells did not round up and instead detached in the form of a sheet....
02 June 2023 1,380 3 View
I am looking for all the possible solution could be implemented to remove sticky PDMS from master mould and with it any necessary precaution needed for avoiding similar situation as shown in the...
01 June 2023 4,794 3 View
I'm trying to do a long-range PCR (~10kb) and I'm just wondering what polymerase to use despite the Q5. Do you have any experience in doing a long-range PCR with other polymerases? Did you use the...
31 May 2023 9,824 3 View
I would like to add an epitope tag to my protein (internal, not C- or N-terminal) for imunocytochemical labelling. Do you have suggestions for specific epitopes / antibodies that may work well,...
31 May 2023 10,004 0 View
Can I become a doctor for special diseases? What kind of field is the biomedical field?
26 May 2023 1,288 2 View