anybody know the reference or housekeeping gene of coconut hispine beetle brontispa longissima for qPCR?
thanks
Hello Habib:
The mtDNA COI of Brontispa longissima can be amplified with the universal COI primers C1-J-2195 (50-TTGATTTTTTGGTCATCCAGAAGT-30) and TL2-N-3014 (50-TCCAATGCACTAATCTGCCATATTA- 30; Simon et al. 1994), and the ITS gene of B. longissima can be amplified with the universal primers 18SRCP (50-TCCGTAGGTGAACCTGCG-30) and 28SRCP (50-GTAGTCTCACCTGCTCTG-30; Huang et al. 2006, Tang et al.2014).
See the following link by Zhang et al. 2015 for more details. https://www.researchgate.net/publication/273181875_A_Rapid_Diagnostic_Technique_to_Discriminate_between_Two_Pests_of_Palms_Brontispa_longissima_and_Octodonta_nipae_Coleoptera_Chrysomelidae_for_Quarantine_Applications
Hope this helps
Regards,
Luis Miguel Constantino
Article A Rapid Diagnostic Technique to Discriminate between Two Pes...
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