Which database can I get the sequence of dgat1 gene or protein of Chlamydomonas reinhardtii ?

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Can anyone suggest a numerical method to calculate perimeter of a contour in charge density contour maps?

Hi i have drawn the charge density contour maps using VESTA taking CHGCAR files from VASP. Now, What I Want is that I need to know the perimeter of each contour appearing in the cross section 2D...

04 February 2021 3,637 1 View

How can I calculate flat band with valence band and conduction band potential for P-type semiconductor from negative slop Mott-Schottky plot?

I have a lot of journals regarding finding out VB and CB for n-type semiconductors from Mott-Schottky plots where MS slope is positive but I cont able to find out VB and CB for p-type...

01 December 2020 6,198 1 View

How can I calculate valence band maxima of a semiconductor using XPS?

I just need to calculate the valence band maxima for a semiconductor which is actually in a heterojunction with another semiconductor. The motivation is to find the exact energy level of VB and CB...

01 December 2020 3,774 7 View

How can I calculate the composition and quantity of different types of calcium aluminates and sulphides from EDS analysis of inclusions in steel?

How can I calculate the composition and quantity of different types of calcium aluminate (CA6, CA2, CA,...) and sulphides (CaS, MnS) from the EDS analysis result of inclusions present in Al-killed...

07 August 2020 4,621 1 View

Do I need to normalise the RNA for qPCR?

Dear all, Do I need to normalise the RNA before making the cDNA for qPCR to find out the absolute quantification in copy numbers? Why? What logic behind it when the primers amplify the...

20 February 2020 5,886 3 View

What kind of connectors and how to connect it to my small patch antenna?

I have designed a microstrip patch antenna that has a dimension of 13 mm X 8 mm. The microstrip line width is around 500 micrometers. The substrate is not PCB or FR4, for some reason, I can not...

10 February 2020 7,478 6 View

Total DNA-PK immunofluorescence on M059J cell?

Is anybody has any experience on doing Total DNA-PK immunofluorescence on M059J cells. As far I know M059J has inactivated or truncated DNA-PK protein. But can it be detectable by IF with Total...

06 February 2020 6,057 1 View

deleted the question.?

----deleted the question.

22 December 2019 4,752 2 View

What exactly we can infer from Charge Density Difference plot of a Hybrid system?

Consider a Hybrid system with two crystalline materials A and B, where is A used as a base (or substrate) and B (say a 2D semiconductor material) on top of A. For this system, a computational...

27 September 2019 4,425 2 View

Problem with the WB-F344 cel line. anyone had any experience ?

for last two months I try to culture WB-F344 cell which was purchased from JCRB cell bank. As mentioned in the documents I cultured them with MEM including 10% FBS, but after 4-6 passage the...

06 January 2019 4,021 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View