Which database can I get the sequence of dgat1 gene or protein of Chlamydomonas reinhardtii ?

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What's wrong with my COMSOL model for turbulent flow in time dependent solver?

I am trying to simulate velocity field inside a domain of just ambient air (pressure is 1 atm & velocity is 10 m/s). There is a tank inside it which has a leakage. The leakage hole is leaking...

01 July 2024 2,249 0 View

How the CBM and VBM plots are made using VASP?

Hi all, I seen CBM and VBM plots in articles plotted using VASP, i pasted a part of the sample picture. I used to do with Gaussian, but can any one explain How exactly these could be plotted...

28 April 2024 5,155 4 View

The Fermi energy cannot be calculated for a bulk material. is it correct or not?

17 April 2024 6,217 0 View

In an L-fuzzy metric space, what is the topology on the Lattice L?

In an L-fuzzy metric space, what is the topology on the lattice L? We have the sequential definition of continuous T-norm in an L-fuzzy metric space. Without the topology, how can we talk about...

29 March 2024 2,401 0 View

Is there any field guide for identification of plants?

Please suggest how to identify the herbs, shrubs and trees in field

25 March 2024 7,172 6 View

Is there any method to isolate DNA from a clot blood sample preserved in RNA/DNA sheild buffer?

For DNA islolation

13 March 2024 7,635 4 View

Assessing the Impact of Urbanization on Agricultural Land?

Hi, "Assessing the Impact of Urbanization on Agricultural Land" I want to use Remote Sensing and GIS as tools to analyze the conversion of agricultural land to urban areas. Can anyone give...

24 December 2023 9,631 2 View

Can we culture the human PBMC with nanoparticle and do the metabalomics studies?

I want to know, can we culture the human PBMC with nanoparticle and do the metabalomics studies? Please let me know

15 December 2023 548 2 View

What is the difference between the items/statements of an attitude scale and a perception scale?

I want to know how to select the items both of these scales? & how should the items/statements of each scale be formulated or phrased?

02 December 2023 2,190 0 View

Mechanism of crystal staining?

Where does the crystal violet bind to when added to live and dead cells? Which part of the live cells they bind to?

09 October 2023 5,577 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

How I can add anew research in my account ?

Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?

21 July 2024 7,545 0 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,405 2 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

How to prepare materials before protein-peptide or protein-protein docking?

Hi all, I am conducting research on screening peptides (20-35 amino acids) with potential for treating dengue fever through protein-peptide docking methods. In the protein database, I found the...

10 July 2024 8,037 1 View