Can anyone help me to identify these Antarctic serpulids at least until genus? I know the photos are not good but if anyone has any suggestion to have an idea, it will be great. Many thanks! Blanca
I've been reading up on how to best represent qPCR data, specially on answers given on this site by Dr. Jochen Wilhelm. I understand box plots are the way to go, but I still don't understand them...
11 February 2021 7,228 3 View
Hi everyone! I'm working on a DNA extraction from serum. Unfortunately the concentration was very low and the PCR amplification didn't work, maybe because the DNA extracted was very fragmented. Do...
23 August 2018 4,155 3 View
Hello, I have fixed and obtained frozen sections of salamander embryos for fluorescence microscopy. The sections are 8um thick and the embryo used for the attached images was kidney bean shaped...
13 February 2017 7,604 1 View
Hello everyone,I am gearing up to prepare my first ImmunoTEM samples via a pre-embedding protocol. The samples are isolated mouse sperm. I searched the literature and I am gathering bits and...
03 March 2016 9,794 5 View
We are using EpiQuick histone extraction kit from Epigentek, but we want to know if anyone can suggest any other kit as well as any recommendation... Although this kit leads obtaining histones...
05 October 2015 7,000 7 View
I run the electrophoresis in a pre-cast 4-20% gel at 200 V for ~45 min, then i've tried to transfer them to nitrocellulose membrane in the following conditions (always in a cold, wet environment):...
01 January 1970 7,229 6 View
I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...
02 March 2021 6,270 3 View
I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...
23 February 2021 1,631 3 View
The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...
18 February 2021 9,538 1 View
Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...
15 February 2021 7,703 4 View
I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...
22 January 2021 585 10 View
I am currently conducting my final year dissertation which is looking at the efficacy of common police lineup practices. However, before I launch my study, I have put out a pilot study which is...
08 January 2021 4,376 4 View
Hello, Please, I need your opinion. I want to map the depth of 20,000 hectares of land. I have sampling points of 20,000 with their respective depth attributes and photos of the topsoil(less than...
31 December 2020 4,325 3 View
I want to calculate an enzyme's energy of activation, but I don't have the enzyme's molecular weight to calculate the rate constant or the pre-exponential factor A. This is part of an assignement...
25 December 2020 8,738 3 View
We see a photos of solar array damage suffered by the ESA Eureca spacecraft that was recovered by the Space Shuttle. photo shows a sustained arc site on its solar arrays. In this case, the...
23 December 2020 2,512 2 View
I performed Tricine SDS PAGE according to the Nature protocol. Included in the gel is 4% stacking, 10% spacer, and 16% resolving. I intended to separate peptides lower than 10kDa. Therefore, I...
16 December 2020 1,578 1 View