The common name for the genus Rattus. | Review and cite RATS protocol, troubleshooting and other methodology information | Contact experts in RATS to get answers
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Questions related to Rats
Hi Im seeking advice on what would be the best model of T2D and metabolic syndrome in rats Thank you
05 September 2020 3,977 4 View
Hello all, Has anyone tried to orally administer ketamine to mice or rats inside drinking water, wihout addition of any sugar? Do the animals really avoid drinking water with ketamine? I tried to...
02 September 2020 717 3 View
I am looking for a source of cDNA primers, rat genome, real time PCR. Previously I used rtprimers bd, but is seems to be out of service for a while now.
02 September 2020 5,452 3 View
Hello everyone, my interest is to understand the molecular mechanisms involved in anaphylactic shock using Wistar rats. While i inject non-allergic naive rats with iv bolus (1 mg ova in 1 ml),...
28 August 2020 9,877 2 View
I am looking for a model cell line to validate anti-rat EpCam Antibodies but i am struggling to find a good candidate. Many thanks!
25 August 2020 528 2 View
Hello everybody, I'm planning to investigate the in-vitro release of different markers (CXCL10, INF-a, IFN-b, 1L-1a, IL-1b, IL-6, free DNA) by NR8383 rat macrophages, upon incubation with several...
21 August 2020 9,049 1 View
Hello, I am trying to find antibodies that can be used in paraffin rat brain sections against S100B , PSD95, Synapsin-I, VGLUT1,VGLUT2. I am checking the technical info provided by some companies...
13 August 2020 5,674 2 View
I'm conducting a toxicological experiment. I administered crude ethanol extract to pregnant rats. I need to check two things -the serum concentration of the extract in the dam rats...
12 August 2020 2,135 2 View
Hi everyone, For my experiments I need to work with the rat pancreatic cell line Ar42J. I have purchased (twice now) the cells from ATCC, however after the process of thawing and transferring...
04 August 2020 5,883 0 View
This is in an in vitro model of skeletal muscle cells where insulin-mediated AKT phosphorylation is increased. Any explanation will be appreciated.
02 August 2020 9,434 0 View
Hello, Does anyone have any experience with isolation of CD8+ cytotoxic T cells and natural killer cells from rat spleen? I'd prefer kit-based if possible, but any protocols you can provide would...
31 July 2020 5,709 6 View
How can chemical laboratory experiments be done on pregnant rats? Upon knowing the effect of a chemical substance, protein, hormonal or enzyme on animals, experiments with pregnant rats .. Do any...
23 July 2020 6,328 1 View
Hi! Anyone knows whether I can performe behavioral tests in rats at PND30 and then, repeate them at PND60? Or this is not correct? Thanks.
21 July 2020 3,917 3 View
Dear Experts, I have studied the magnetic properties of a perovskite cobaltite and found that Curie-temperature (TC=147 K) is decreasing and Curie-temperature (TCW) is increasing with an...
21 July 2020 4,009 4 View
How do I use deamethasone to supress rat Hypothalamic-Pituitary-Adrenal Axis? What is the dose normally used (eg. 3 mg/kg once or repeat for many dayds.....) and how long the supressed effects...
18 July 2020 4,243 2 View
potassium oxonate is used to induce hyperurecemia in rats . It has effect on relative kidney weight .
14 July 2020 5,958 3 View
I am looking for a reliable and sensitive AMH ELISA for use with rat serum (and potentially plasma). Recommendations are welcomed! Thank you in advance.
09 July 2020 3,499 2 View
Hello, everyone! I have been working with this model in my thesis protocol. I wanted to ask you if you have ever let your mice living after the administration of acetic acid 0.6% for more than 24...
05 July 2020 6,462 0 View
I am asking whether there are any differences between mouse and rat microglia. For example the density, flow cytometry isolation protocols, number of isolated cells, surface markers, antibodies,...
04 July 2020 1,946 3 View
We have IVIS lumina 2, which is currently having a problem of camera not being cooled to recommended range of -90C. What happens exactly if it does not cool to -90C? My understanding is that it'll...
01 July 2020 4,389 1 View
Rat heart tissue, I am assaying for CSE/CTH protein expression always give a triplelt bands at 72,56 and 45Kda (Published data only showing a single band detected at 45Kda in heart tissue). The...
25 June 2020 5,035 3 View
I need an automated system to help me count the total number of blood vessels and total number of muscle fibers (Cross section). It will be used for large muscle (Lateral and Medial Gastrocnemius)...
23 June 2020 442 0 View
I am doing a baseline + 2 rounds of behavioural testing (NOR + NLR) - so total 3 rounds of testing, all 1 week apart in male SD rats. Is it necessary to habituate them before every test, or is...
18 June 2020 9,343 5 View
Hi all, My lab has been working with Athymic Nude mice in order to test anti-cancer compounds in mice with tumor xenografts. The main cell line we work with is the epidermoid carcinoma line...
16 June 2020 7,705 0 View