How can one explain a selective increase in Glut1 mRNA levels while Glut4 remain unchanged?

02 August 2020 0 9K Report

This is in an in vitro model of skeletal muscle cells where insulin-mediated AKT phosphorylation is increased. Any explanation will be appreciated.

Badges
Science topic

Similar topics
Rodentia
Muridae
Murinae
Rats

More Dipsikha Biswas's questions See All

Is there any cracked version available TuFLOW software? How to get tutorials of this software?

Is there any cracked version available TuFLOW software? How to get tutorials of this software . As i want to use for modified shallow water equation in urban flood modeling and simulation

06 July 2024 2,741 0 View

There are any alternative software Infoworks or Mike for urban flooding?

please suggest if there are any cracked versions of these software available.

04 July 2024 8,704 2 View

Which solvent and substrate is found to be best suited for deposition of electrodes with MXene ink?

Suppose we have dried MXene slurry (Ti3C2Tx), then the combination of which solvent (in which this dried MXene slurry powder will be mixed, subsequently forming the "ink") and corresponding...

13 June 2024 5,763 0 View

Do you think academia, particularly in the humanities and social sciences, is a continuum of semantic tricks?

Every concept feels like its predecessors stated it more precisely, with successors just adding context. It's rewording, but the understanding stays the same. For example: Structuralism, as...

10 June 2024 3,979 0 View

Can the aims outlined by a translator in the preface be understood as Skopos?

Skopos theory, developed by Hans Vermeer, is a functionalist approach to translation that emphasizes the purpose (Skopos) of a translation as the primary factor guiding the translation process....

02 June 2024 761 3 View

Which institute in India has Intelligent gravimetric analyzer for hydrogen gas storage?

16 May 2024 9,115 0 View

What type of validity is measured for questionnaires by Product Moment Pearson Correlation in SPSS?

Product Moment Pearson Correlation was done in SPSS for the validity test by correlating each item's questionnaire scores with the total score. What type of validity (content/ criterion/ construct...

27 April 2024 4,753 2 View

How can we induce drought stress in rice plants in vitro? What methods measure stress( MPa) accurately?

This question seeks to explore the techniques for inducing drought stress in rice plants in laboratory conditions and methods for accurately assessing the resulting MPa stress levels. It aims to...

17 March 2024 5,840 0 View

How to create a map of strip cultivation?

I want to create a map of specific cultivation, which is very narrow. It is not possible to identify in satellite image with 15 m resolution and also not possible to digitize on Google Earth Pro....

25 January 2024 2,818 2 View

Why there are traces of fluoride particles in my supernatant while synthesizing MXenes (Ti3C2Tx)?

I am trying to synthesize MXenes (Ti3C2Tx), using the MILD etching method (in-situ formation of HF by reaction of LiF + HCl). I am taking equal amounts of LiF powder (Sigma Aldrich, >99.99%...

24 January 2024 986 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View