The common name for the genus Rattus. | Review and cite RATS protocol, troubleshooting and other methodology information | Contact experts in RATS to get answers
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Questions related to Rats
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Hello. I am isolating single nuclei from snap-frozen samples. The RNA integrity from whole tissue/bulk RNA extraction is good with clear 28S/18S bands on bleach gel. However, after performing...
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This is what the head of the Israeli National Security Council said: There is a long period of time to eliminate Hamas, and after this time, which is nearly a year
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Hello, I have cross-sectional data on immune cell density in a disease group. Compared to controls, the disease group shows an increase in immune cell density. Now, I want to run a clinical...
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Hi guys, I am running WB on JESS (ProteinSimple) targeting p65 (NFkB) and we are, for some reason, detecting only a 46kDa band in the liver tissue. This is the cytosolic portion of the...
17 June 2024 4,846 0 View
I have been using the Lonza nucleofector for RNP delivery to OT-I T cells without any issues for the past six months. However, recently, my nucleofected OT-I T cells have not been proliferating in...
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Hi, I am implanting G2P10 GRIN lenses for 2P Imaging, Ø1 mm and 3.400 mm long, to PRL mPFC; DV:-1.7. After the implantation I used superglue really carefully around the craniotomy and the lens in...
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Can Opti-MEM without serum be used for transfection? Myotubes are typically at a high density upon successful differentiation. Does this reduce the efficiency of transfection?
16 May 2024 5,964 0 View
Hi, which easily transfectable cell lines respond to poly (I:C) (ie by IP10 induction). My 293 cells don't seem to! I appreciate I need to transfect poly (I:C) if the cell line is deficient in...
13 May 2024 10,063 0 View
I am analysing data from hippocampal cellular models I have 12 conditions and 3-6 technical replicates and 3 biological replicates. I have a few questions, what correction method do I use for...
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I recently did FRAP and FLIP experiments in a different lab. In FRAP: I treated HuH7 cells with different inhibitors. The import seemed not influenced, but of one inhibitor the resulting...
17 April 2024 1,459 0 View
How can I delete a research publication
27 March 2024 7,910 1 View
In my study, I have a within-subject independent variable (the DV was measured twice for each participant), and I am also interested in examining the effect of participants’ demographic...
15 March 2024 9,177 3 View
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02 February 2024 1,607 1 View
My study requires the induction of STZ in 30 Sprague-Dawley rats (either sex) to develop type 2 diabetes. My protocol was: 1) Fast the rats overnight. 2) Prepared STZ solution in ice-cold citrate...
23 January 2024 7,287 4 View
I have done an RMSD validation for such protein by docking the two ligands one by one, but each docking results in large RMSD value compared to the reference.
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When undertaking clinical trials with limited sample size and repeated measures, the question arises: which is more advantageous—employing "Generalized Estimating Equations (GEE)" or opting for...
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Suppose, I have daily data for a certain time series variable. However, if I want that variable to use quarterly data for research, how should I organize the data?
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I am performing stereotaxic injection of an AAV8 serotype into mice and then immunofluorescent staining for target antigens in free-floating brain tissue. The surgeries appear to go very well and...
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Hi everyone, I'm part of a team keen on developing a deeper understanding of protein interactions within a cellular context. Our primary goal is to identify and implement the most effective assay...
22 January 2024 2,038 2 View
I am staining for VGLUT2 in mouse brain sections. I am seeing a lot of debris on the slices and these are visible in both Green and Red channels. By looking at the sizes I am sure that these are...
18 January 2024 1,426 0 View
I want to distinguish neutrophils from other cells in PFA-fixed and paraffin-embedded tissues. Could you provide recommendations for a basic histology staining method, other than H&E staining,...
17 January 2024 4,433 4 View
I need explanation of metaanalysis of subgroup analysis
03 January 2024 3,210 0 View