The common name for the genus Rattus. | Review and cite RATS protocol, troubleshooting and other methodology information | Contact experts in RATS to get answers
2,382 views 615 posts
Questions related to Rats
I have been looking for ViBo cervical cancer cells information in the ATCC but I can´t find nothing about it. I ask for help if anypeople know about an article or web site for consult ViBo cells...
11 January 2023 7,344 3 View
I want to evaluate wound healing of a drug-delivery system in rats. According to the ethical standards for the use of animals in research, it is recommended to use a minimum number of animals to...
29 December 2022 5,409 2 View
I am curently working on primay neuron cell culture and i read that D-arabinofuranoside inhibits the glial cells. Any other compounds that can inhibit the division of glial cells?
18 October 2022 4,575 1 View
Hello, I'm working on senescence and I'm inducing it using TNF alpha. I would like to assess the induction of the phenotype looking for the cytokines typical of the secretome. Which cytokines do...
20 September 2022 2,159 3 View
I am trying to measure autophagy flux in RPE1 cells by measuring the LC3II-LC3I ratio. I would like to induce autophagy using spermidine while inhibiting lysosomal digestion using bafilomycin to...
31 August 2022 2,658 0 View
I would like to find an anesthetic that has the least effect on coagulation factor. Thanks
03 June 2022 5,402 9 View
I´m using Leica VT1000 vibratome for slicing rat´s cerebellum for patch clamp experiments. Which parameters are better to use? Thanks
25 October 2021 9,517 1 View
Grid-walking test refers to the method to assess the locomotion accuracy of a rat/mouse as described in PMID: 22142899. It's an ardous work to stop the video recordings again and again to count...
19 October 2021 4,983 1 View
I am asking about a detailed protocol.
13 October 2021 4,253 4 View
I'm recently working on anthelmintic activity in vivo on rats for which I need to induce helmenthiasis in rats can anyone suggest me that how should I grow parasite
04 October 2021 2,731 3 View
Best module for breast cancer induction.
29 September 2021 4,141 3 View
What is the best approach to induction of anemia in experimental rats?
16 September 2021 4,056 4 View
I couldn't find any anti-rat CD20 which has been tested for in vivo B cell depletion in rats. Could any anti-rat CD20 or CD-19 ab work for B cell depletion in rats?
16 September 2021 4,716 2 View
Hi, I am interested in chemogenetically manipulating GABA cells and came across 2 genetic rat lines: VGat-IRES-cre ad Gad1-cre rat lines. I was wondering what the difference between these two...
09 September 2021 8,497 1 View
Hello! I am having problems in the cultivation of brain microvessel cells. I isolated cells from adult rats. In cultivation, I used 4 μg / ml puromycin for 2 days. (1 image) I have 3 problems:...
08 September 2021 9,346 1 View
I have done beta gal assay in mcf10 A cell line. I am not familiar with the result. Would you please let me the know that whether the uploaded cell image are showing senescence or not?
06 September 2021 673 3 View
I am planning to perform in vitro electrophysiology experiments on rats model. Is it possible to store whole rat brain for invitro electrophysiology experiments or one have to perform the...
04 September 2021 5,967 2 View
Hello, I have to administer antiplatet drugs, such as aspirin, clopidogrel, prasugrel, ticagrelor to rats and I am thinking of oral gavage as the preferred route. Which you consider to be the...
03 September 2021 4,215 2 View
My recent results indicate a decrease in the expression of beta-catenin in rat cardiomyocytes in response to a specific drug and in-silico model of binding energies between the said drug and...
30 August 2021 7,835 1 View
As we know that Xylazine/Ketamine administration, leads to acute hyperglycemia and subsequently a decrease levels of plasma insulin in fasted rats and this count as sampling error in experiments...!
21 August 2021 4,433 6 View
I have done some studies on DM rats and rat pancreatic acinar cell line AR42J. I would like to extend my research to alpha cell line to see the glucagon secretion. However, I can only find mouse...
10 August 2021 7,994 1 View
Hey, I am analyzing rat chondrocytes via flow cytometry. unfortunately, I treated them to become senescent and they secrete something viscose. How do I get rid of that, not hurting the sample?
06 August 2021 9,988 2 View
Hi, I'm doing western blot for phospho-RhoA using rat brain tissue extract. But I'm getting double bands around the right molecular weight, and they are very close. Is there any idea of what...
04 August 2021 2,217 4 View
I used this kit for measuring Total TG content in rat Plantaris muscle. However, I have some questions about the calculation of the final TG concentration. It is highly appreciated if anyone can...
30 July 2021 4,399 2 View