How to perform a Qualitative Analysis of Polarization on Twitter ?

Hi, I want to study the increasing polarization on social media. I have to use a qualitative method to do so. However, I am more familiar with quantitative methods. Hence, I am struggling to find...

15 January 2021 2,426 3 View

Using different scales for intra-organisational findings?

Hi all, I am trying to assess 3 unrelated factors in our organization. Together, they should give me an index of how well our workers in different countries are prepared to continuously learn...

22 November 2020 3,495 3 View

Have you tried the Tissue Total RNA Mini Kit, with DNase (50) rxns from Geneaid?

We are going to work with very small tissue samples and we are looking into different kit from many brands and this one is significantly less expensive, but we haven't heard about it. Has anybody?...

18 November 2020 1,387 1 View

Troubles with cryosectioning (frozen sections) on skin tissues?

Can someone help me with some cryosectioning issues? I made skin tissues in vitro and my protocol is already validated. I've always go for the paraffin cutting but is currently out of service....

05 October 2020 4,339 10 View

Comparing correlated means in a t-test?

Hello, I have a categorical variable (main teaching assignment) with 12 options (early childhood, mathematics, English, etc) and I am trying to determine which groups earn significantly higher...

08 September 2020 5,046 5 View

How do I interpret my AGE results?

Hello! I am in need of help analyzing my results from Gel Electrophoresis. The first photo indicates that there was DNA extracted but did not go down. The second one shows that the DNA has been...

07 August 2020 6,125 3 View

Interaction-effect with categorical dependent variable in SPSS?

Hi there, I am struggling with my thesis. I need to test in SPSS if there is a significant interaction effect. I have four groups, and they all fill in a survey. The only difference of the...

27 July 2020 6,074 1 View

How to avoid loss of solvent using Dolomite Microfluidics System and maintain stable flow throughout the run?

Hi everyone. I'm currently working with Dolomite Microfluidic system for preparation on PLGA based microparticles, but I seem to be encountering some issues which have been giving me some trouble...

29 June 2020 827 1 View

After running Chi-square test for comparison between 4 groups, is there a method of checking which groups differ significantly with nominal data?

My data is nominal/categorical. I want to see if 4 groups differ significantly. The question for them is if they convict a person or if they acquit the person. So the data is categorical: convict...

08 June 2020 5,208 7 View

Is anyone aware of datasets published in the grey literature about diversity of pollinators of apricots?

I am looking for research, which is maybe not published in English or in peer-reviewed journals.

28 May 2020 2,962 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View