The common name for the genus Rattus. | Review and cite RATS protocol, troubleshooting and other methodology information | Contact experts in RATS to get answers
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Questions related to Rats
I am working on the determination of tulathromycin in rat faeces using QE-focus (Thermo Fisher)(Waters BEH C18, 2.1x100mm, 1.7um)(0.1%FA+H2O, 0.1%FA+ACN) . I encountered two tricky problems....
03 January 2021 3,046 5 View
Hello, I'm using a Rabbit Glucagon antibody from Abcam to stain human primary islets and iPSC derived islets for Glucagon along with other pancreatic cell markers such as PdX1 and C-peptide. The...
29 December 2020 1,509 0 View
When multiple vendors supply equipment, who determines what information is available to the owner and the BACnet operator workstation? Is all the information available to the BACnet operator...
22 December 2020 7,100 0 View
Dear, We developed a microcarrier that was pre-seeded hUC-MSCs to repair a rat bone defect. However, we didn't use GFP or Luciferin to track cell survival. If there's any way to identify the...
16 December 2020 6,315 3 View
Is anyone able to tell me why my anaesthetized rats often do a 'double breath', or diaphragmatic breath, like a hiccup (see video). This often begins after 1-2 hours under anesthesia, and can...
07 December 2020 2,751 5 View
I am looking for an anti-rat CLEC-2 antibody for IHC in rat lungs. Has anyone come across one? There are a few mouse ones but the data sheets don't say they cross react with rat, and the ones that...
07 December 2020 3,753 0 View
I have 1L of hybridoma supernatant without serum from a rat monoclonal antibody. I would like to purify it using a binding affinity column for rat kappa chain. Could you please tell me where I...
30 November 2020 3,544 3 View
The antibodies to human sexual hormones are much easier to get than antibodies to rat sexual hormones. So, the question is raised how correct is application of antibodies to human sexual hormones...
21 November 2020 5,328 1 View
Hi, I am interested to know the DNA sequence of the TF corrisponding to the DNA binding domain. Do you know any tool developed recently that can help?
15 November 2020 9,761 6 View
I recently switched lot of a primary antibody used in a competitive RIA. I have performed a titration experiment and the new antibody now must be used a a 1:1M dilution to give the same maximum...
11 November 2020 5,096 0 View
The rats are perfused with PBS and the brain sections are 18 microns thick and fixed on slides.
10 November 2020 1,029 3 View
Dear colleagues, I would like to ask you if anyone has experience with the fixation of whole rat organs (heart, lungs, liver, kidneys, brain, testes) in 10% buffered formalin. Thank you, Roman
29 October 2020 3,416 3 View
How should I measure motility, what is the process? Should I put it in the incubator (37 C) immediately after sacrification? Thank you from now.
29 October 2020 503 4 View
how can i collect many blood sample from the rat for pharmacokinetic study without killing the rat??
29 October 2020 2,942 8 View
I'm working on a mesenchymal stem cell culture isolated from rat bone marrow and I want to yield a specific MSCs count from passage 3 . I want to know the yield of cells in passage 3 to know how...
28 October 2020 615 1 View
We need to collect CSF samples repeatedly from the 4th or lateral ventricle of rats. Does anyone know of a protocol for implanting a cannula to be able to do this? Or even a protocol for this...
27 October 2020 6,059 3 View
I am looking to study neurons of the bed nucleus of the stria terminalis. I have seen reports using coronal sections. Others present the distinct nuclei in transverse sections. What is the best...
23 October 2020 3,552 1 View
Hello every one, i´ve been working with rats for a few years but now i'm wondering wether there is a way to calculate the BMI or the adipose tissue in these animals. Also, i've been searching...
23 October 2020 3,001 5 View
I want to know that in which quantity if at all needed we should add sodium sulphate to cattle feed, so that we can see increase in milk production and reduction in methane production. Also in how...
22 October 2020 4,764 4 View
Our lab is trying to establish a BT-474 xenograft model. We have not been able to get the tumors to grow past 50-100mm3. We inject 1x107 cells in a 50:50 matrigel suspension. We used nude mice (5...
21 October 2020 2,846 4 View
Good day house. Please I intend to start exposure and treatment in Rats and Mice on the first day of pregnancy. So how do I determine the first day of pregnancy which is always designated as...
16 October 2020 7,615 4 View
I want to measure the levels of lipopolysaccharide (LPS) in rat serum and if possible, rat brain tissue samples. There are a lot of kits out there that can measure LPS in serum but I wasn't sure...
11 October 2020 319 0 View
I have a problem about rat anesthesia, the situation is that I have Zoletil 50 1mg/ml + xylazine hydrochloride 23.32mg/ml solution that I prepare at the same time from the same source (from the...
08 October 2020 9,349 7 View
Hello everyone, I need help from you with my CD31+Dystrophin analysis. The question is: How to analyze the Capillary Density and Capillary-to-Fibre-Ration in skeletal muscle? Any tips when you...
08 October 2020 828 0 View