The common name for the genus Rattus. | Review and cite RATS protocol, troubleshooting and other methodology information | Contact experts in RATS to get answers
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Questions related to Rats
Which dose of stz (except 3mg/kg) is better for induce Alzheimer in fast time with minority rat of death in male wistar rats through icv induction ?
20 August 2023 713 0 View
Which dose of stz (except 3mg/kg) is better for induce alzheimer in shorter time with minority rate of death in male wistar rat through icv induction
20 August 2023 9,215 6 View
We had some male wistar rats and we tried to induce stz( 3mg/kg)through icv induction but after few days they showed mortality signs such as feet and hand paralyses and nose and ears bleeding and...
20 August 2023 4,056 0 View
I need to cultivate endothelial cells (ECs) under low or no glucose conditions. Usually I culture ECs using endothelial basal media (EBM) or endothelial growth media 2 (EGM2). But I can't remove...
25 July 2023 1,365 4 View
Hello everyone, I am in the doing viral injection (hamilton and automatic pump) with AAV-mcherry targeting piramidal neurons in mouse aging from P40 to P50. After 2 to 3 weeks, I performed...
11 July 2023 7,699 2 View
I'm interested in somatosensory metabolism in a model of cortical malformation with susceptibility to absence seizures.
14 June 2023 7,826 1 View
Hello there. I just completed an experiment using both hM4D(Gi) and hM4D(Gq) DREADDs in the cerebellar vermis, both co-expressed with mCherry. I noticed that while I had a lot of expression of the...
31 May 2023 4,602 1 View
Hi, Does there has anyone know the working PH range of the lyso-tracker probe? I want to distinguish secretory granules and lysosomes for analyzing their interactions, but I just realized that the...
29 May 2023 8,259 2 View
I need to perform cytotoxicity studies with an isolated substance for a future formulation with oral administration. However, I don't know which cell lines to choose to test cell viability......
06 May 2023 3,829 4 View
I think of applying the drug as a continuous infusion over 36 hours. I thought of putting it on a bio-compatible membrane over the surface of the brain is this possible? has anyone seen a study...
27 April 2023 8,537 0 View
Thanks.
27 April 2023 8,203 2 View
I am working with a protein whose structure is not available in PDB, and I wanted to know if it is possible to find the protein residues which are RNA binding.
01 April 2023 8,903 5 View
For e.g in antimicrobial studies, I want to carry out an assay using "Methanolic extract" of a plant sample. Is it advisable since methanol as a solvent possess some antimicrobial properties? Will...
26 March 2023 5,694 6 View
Please, how can I sterilize a nano preparation if it is affected by light, and it is in colloidal state and may lock the syringe filter? Later I have to study its effect on cultured cells....
19 March 2023 1,042 1 View
I am trying to do some immunofluorescence on mouse testis sections but after staining and mouting, the tissue seems to degrade rapidly and becomes smudgy, in particular nuclei. Has anyone...
22 February 2023 7,262 2 View
I am working on the Chaperone mediated autophagy and presently working over the isolation of lysosome from HEK cells. I am able to isolate the lysosomes but their inetegirty is very low when it is...
20 February 2023 5,070 1 View
Hi I have a membrane that has a a protein inside it. I want to know how can I put another protein in this membrane and check their interactions. I would be thankful if you help me by explaining...
17 February 2023 6,327 6 View
After contextual fear conditioning + 4-OHT injection I can see dtTomato-positive cells in several regions of the brain (mice are sacrificed 4 days later) but nothing in the dorsal CA1. Can someone...
14 February 2023 7,878 0 View
If i start clustering in k-means for some data using initial centroids, lets assume 10 centroids to create 10 clusters, is it possible that it will end with 8 or 9 clusters?
14 February 2023 8,807 6 View
Apart from the following: aging atlas, human aging genomic resources, agefactDB, the digital aging atlas, AGEMAP, genage, gisao.db, gendr, longevity map, drug-age, cell-age, CSgene, open genes and...
08 February 2023 498 0 View
Hello everyone, I want to concatenate about 20 files, but It shows an Error “one starts earlier or overlaps than another”, so they can not concatenate, I only can have 10 of them in the new...
07 February 2023 8,235 1 View
I Wonder if there is any study to compare different rodent responses validity in "hot plate" test. Thanks A lot
01 February 2023 5,691 0 View
Hello everyone, i hope you all are doing well, I have performed docking study with some selected molecules along with the reference inhibitor and the docking results were for selected two...
31 January 2023 7,367 1 View
Hi, I am running westerns on adolescent mouse hippocampal tissue and I am struggling to identify the correct band for Mouse MAP2/cd. Attached below is a snapshot of westerns with two different...
24 January 2023 3,984 2 View