The common name for the genus Rattus. | Review and cite RATS protocol, troubleshooting and other methodology information | Contact experts in RATS to get answers
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Questions related to Rats
Hi, I'm trying to induce cellular senescence in HaCaT cells. I tried H2O2 and then tried to check the cellular senescence using S A beta gal assay. Unfortunately I didn't succeed to get SAbeta...
28 May 2021 4,796 4 View
I'm going to analyze GSK-3 concentration in rat hippocampus homogenate but I don't know how correctly should I calculate this concentration per total protein level examined by Bradford method?
18 May 2021 7,335 1 View
Hi everyone, I'm currently studying an MSc in drilling and well engineering, however, I'm struggling to come up with my project proposal for my final thesis. I really like the use and...
18 May 2021 9,953 3 View
I am performing intravitreal injections on postnatal day P2 or P3 neonatal rat pups with an AAV vector and wondering if anyone can comment on how the rats tolerate injections in both eyes as...
14 May 2021 8,774 3 View
We are searching for the best accessible vein, and an approach without anaesthesia.
29 April 2021 10,050 4 View
I was wondering if it would be better to treat the rats in during their most active time
27 April 2021 6,991 1 View
Hello! I want to isolate RBEC from a rat, I used the protocol - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4208856/. However, I used 5-day old rats instead of adult rats. Subsequently, the...
26 April 2021 9,353 2 View
rAAV2/5 even though it is expressing the fluorescent tag in vivo, in the rat cortex, it is not working at all in the primary cultures.
17 April 2021 4,890 1 View
Usually skeletal muscle after injury undergo regeneration process following by all key phases of degeneration, inflammation and so on so until regeneration of fibers. For example we usually...
14 April 2021 2,091 2 View
Can we inject sumanirole maleate intracranially in rat?
13 April 2021 9,623 1 View
I have Started Working on Diabetic Rats and I need to assess the activity of below mentioned Enzymatic activity Hexokinase Aldolase Phosphoglucoisomerase Glucose-6-phosphatase Fructose-1,...
07 April 2021 6,538 3 View
I mean a coating with LPS on glass coverslips to perform immunocytochemistry through cell adhesion upon LPS. I can't find anything in literature. I can only find LPS coating on plastic wells for...
05 April 2021 2,137 0 View
Dear colleagues in a study which aims to test the effect of a medicinal plant on obesity, I want to know if there is a protocol for rapidly inducing obesity in rats.
03 April 2021 6,759 7 View
Hi. I had done a non probability survey to collect information on retirment planning among workers about to retire and those who had already retired. I now want to compare the two populations...
29 March 2021 5,039 3 View
Hey everyone, I am working with a pancreatic cancer cell line which forms tumors in the pancreas in the orthotopic mice model and the same cells form zero colonies in the soft agar assay. I was...
29 March 2021 6,314 0 View
Hi all, Recently, I am trying to measure LTP signal, but I cannot detect the response. I sliced the brain horizontally with vibratome. For slice, i used ice-chilled aCSF (0.5 mM, CaCl2), and the...
25 March 2021 6,829 3 View
I want to know how many isolated rat pulmonary artery rings, I can get from a rat, for use in the organ bath. So how long is a rat's main pulmonery artery. Thank you.
23 March 2021 2,966 1 View
For my experiment, we are examing the effects of estrous stage on heroin withdrawal enhanced fear learning in rats, and for several of the test days we would like to acquire the vaginal cells by...
22 March 2021 9,869 1 View
Toxicology studies say, Ethanolic extract of A.calamus even at a higher dose(3gm/kg) there is no toxicity which is done on adult rats. How to fix therapeutic dose for infant rats.
20 March 2021 8,568 6 View
I am currently trying to perform co-culture of Astrocyte/Schwann cell (primary culture), for Immunostaining I have to use antibodies specifically recognize each cell type, to do so I have been...
11 March 2021 3,868 0 View
We would like to categorize anxiety-like and locomotor activity from rats tested in the elevated plus maze and in open field, for correlation analysis with gut microbiota. Thus, we are looking for...
04 March 2021 7,119 4 View
Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...
23 February 2021 887 1 View
I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...
16 February 2021 3,112 2 View
My thesis involves experimental study with Wistar puppies. I am dividing the puppies equally in relation to the groups. However I only have 4 mothers and I will use all of their puppies. There is...
26 January 2021 7,257 3 View