How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How the precipitation Ratio and flow Ratio effect the output of the result?

I am doing HEC HMS calibration than i want to increase simulated value and volume of the computed so that my issue is how the precipitation and flow ratio make influential that result?

22 January 2021 152 2 View

How can a researcher searching in a new field understand the new field without taking a long time?

By that I mean, for example, I was a researcher in solar cell materials, and currently, I want to research the study of evaporation in soil and water, i.e. in the processes of solving water...

10 January 2021 6,499 4 View

Bio Rad CFX96 Touch Real time PCR System?

Dear friends I would like to ask about your experience with the instrument (Bio Rad CFX96 Touch Real time PCR System) and its performance. Also I would like to know if this version need...

29 November 2020 2,166 5 View

How can I simulate the algorithm for a IEEE802.11ac MAC layer that can optimally reflect the performance evaluation and analysis?

How can I simulate the algorithm for a IEEE802.11ac MAC layer that can optimally reflect the performance evaluation and analysis?

08 November 2020 2,492 2 View

Original DECLUE python/java/c++ implementation?

Anybody working on DENCLUE/improvements? I need to know about any "working" implementation of simple original DENCLUE algorithm.

25 October 2020 3,471 2 View

Is there an implementation of original DENCLUE available?

Does anyone know of a WORKING implementation of the original DENCLUE density based clustering algorithm, and NOT its extensions? I have explored a few from github but they ain't working.Moreover,...

01 September 2020 8,917 1 View

Can anyone share UMATHT subroutine?

I want to see the format of formulation in your code, so i can write for my own problem. U, DUDT, DUDG(NTGRD), FLUX(NTGRD), DFDT(NTGRD), DFDG(NTGRD,NTGRD)

04 August 2020 5,268 4 View

What is the possible way to find out the common antigen in coronavirus?

As COVID-19 has shown the strain variations, various mutants, if we developed vaccination at one place, r we sure that perticular gene has not mutated take example of E-gene of coronavirus.

27 July 2020 9,934 3 View

Does society influence on language or language influence on society? How?

The public question is to all experts of linguists, applied linguists and sociolinguists. This helps to figure out the impact of society in language and the impact of language in society.

28 June 2020 3,048 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View