Recommend antibodies that works in paraffin sections for synaptic and astrocytes markers in rat samples?

More Mar Muñiz Moreno's questions See All

Do you think the people can change his activity behavior just because have a chronic disease?

Along several years specialist study the physical activity, bur why not everybody meke changes.

03 December 2020 2,191 3 View

I would like to ask some questions about concentrations of Urea 3M and 8M in two experiments?

I would like to ask if anyone knows if concentrations of Urea 3M and 8M can interfere with protein purification at Ni-NTA and Glutathione Sepharose 43 resins. Another question, Urea, at 3M and 8M...

17 November 2020 5,014 3 View

I would like to ask if anyone among you knows what is the ideal concentration of TFA to use at a chemical synthesis of peptides?

During a chemical synthesis of peptides by the F-moc method, the following happened: all the initial steps of the synthesis occurred as planned, but in the cleavage, TFA is used. In the lab, there...

02 November 2020 2,997 8 View

How is vertical agriculture progressing in Colombia?

Vertical agriculture offers an innovative solution to reduce the pressure of cultivation on soils. The intensive use of machinery and large areas of land harm biodiversity.

28 October 2020 8,773 3 View

Innovation, Ideas permeability. Which factors make an idea to be commercialized?

There are a lot of new ideas in different fields, but only few will be innovative (new+comercialiced). As an example, Escalators and moving walks have the same mechanical design from more than...

13 July 2020 7,807 12 View

What is the minimum viral load that can be detected by TEM or SEM?

Imagine SARS-COV-2 in a surface to be recovered with an hyssop .

08 July 2020 1,193 3 View

Any good manual for phenomenological approach to sustainability?

I'm thinking about a turn from my ethnographic approach to a phenomenological approach in the study of sustainability and forest preservation. However, I didn't find any good manual to design and...

06 July 2020 9,618 1 View

Are there someone thinking about a simulation software for evacuation/circulation tasks including COVID-19 propagation?

I think it would be interesting to implement the propagation of the COVID-19 virus in some simulation software for the circulation of people, for a population diverse in ages, heights, etc.,...

30 June 2020 7,481 14 View

COVID-19, Lessons learned ?

It should be mandatory to learn from what life teaches us. With COVID-19, we have been forced to buy sanitary materials because in our country there was no industry to do so (dependency) and we...

30 June 2020 3,018 36 View

How to extract polygons of species distribution from books?

If you need a distribution map from a book, how do people deal with that? Do u email authors to obtain the layers or is there a way to export the polygons from the book images? Colleagues and I...

20 June 2020 4,391 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View