Can you recommend a reliable AMH ELISA for rat serum/plasma?

More Anne Marie (Mulligan Tuttle) Gannon's questions See All

Non-parametric version of the wo-way repeated measures ANOVA?

Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...

24 July 2024 3,284 6 View

How to determine the acceptable amount of DMF when synthesizing MOFs?

I have synthesized MOFs already. However, I need to scale it up to produce 100g of MOFs. During my calculation, I need 15L of DMF just for this synthesis! So I thought, maybe DMF is just used as a...

01 July 2024 1,905 1 View

Should there be a difference in biotin management of Alopecia if the patient is a chronic energy drink user?

We have bumped into this energy drink, Celsius, with 1000% of RDA. I am starting to collect some information about this, but there is paucity of information.

08 June 2024 4,733 0 View

Is it normal to wash metal-organic frameworks with DI water for a week?

I have synthesized MOFs and been washing for about 2 weeks now until I reach the goal of UV absorbance of near 0.

27 May 2024 2,295 0 View

Does anyone have experience with AML/T cell cultures in serum-free medium?

We want to keep AML cell lines together with T cells in serum free medium for about 4-7 days. Currently we culture them in RPMI 1640 with 10% FCS, so something resembling that would be good I...

22 May 2024 1,763 0 View

How do you wash the modified biochar?

I usually wash it with MeOH by stirring (10 min.) then centrifuge at 8000rpm (10 min.) followed by DI water washing by stirring (10 min.) and then centrifuge again. I do it for 3x. The final one...

12 May 2024 8,835 0 View

How to look for Special Education MA or PhD Graduates that can validate a Questionnaire?

I am currently working on my Thesis proposal and I am looking for the best way to approach MA or PhD professionals in the field of Special Education. To be specific, I will determine level of...

06 May 2024 5,879 4 View

What are your free yet efficient go-tos tools for meta-analysis?

Need advice on user-friendly tools to ease meta-analysis process of selecting eligible and fugitive studies

02 May 2024 2,332 3 View

How to separate cells from gels?

Hi all, I have a set of cell culture samples embedded in 1% agarose. They have been cultured for 14 days. They have been fixed with 4% PFA. I am wondering if I can separate the whole cells from...

26 April 2024 6,030 1 View

Where does conceptual framework/theoretical framework fall in a grounded theory (Strauss and Corbin)?

I am writing my research using the GT of Strauss and Corbin. However, typical research templates always have this Conceptual or Theoretical Frameworks.

09 April 2024 3,076 1 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

What can be the minimum number of animals (rats) to be used for a pilot/preliminary study on intradermal drug delivery?

Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?

02 August 2024 4,570 1 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View