The common name for the genus Rattus. | Review and cite RATS protocol, troubleshooting and other methodology information | Contact experts in RATS to get answers
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Questions related to Rats
I am trying to measure autophagy flux in RPE1 cells by measuring the LC3II-LC3I ratio. I would like to induce autophagy using spermidine while inhibiting lysosomal digestion using bafilomycin to...
31 August 2022 2,717 0 View
The location of the rat's flocculus is relatively deep and it's very difficult to operate on them.So I would like to ask for more details about the flocculus injection. Could you please give me...
29 August 2022 1,791 0 View
Dear Colleagues, I often use Dry Needling (DN) to treat Myofascial Syndrome with is very frequent in Chronic Pain. Some of the patients are treated with acetylsalicylic acid (ASA). Is this...
15 August 2022 1,108 3 View
We are aiming to use vit. c as antioxidant in quails. we are trying to set the effective dose for it. last papers used vit. c mixed with food for quails or vit. c for rats by gavage. How can we...
29 June 2022 9,055 0 View
We have designed two HIIT swimming protocols for elderly rats. During the pilot we found that the rats are not able to perform the protocols based on the percentage of their body weight because...
18 June 2022 9,084 0 View
I would like to find an anesthetic that has the least effect on coagulation factor. Thanks
03 June 2022 5,464 9 View
Hello everyone! I'm trying to calculate muscle fiber area of a sample but no with immunofixation, only H&E. I'm having a tough time trying to separate each muscle fibers using BZ-X analyzer....
27 May 2022 1,683 1 View
I conduct pressure myography experiments on mesenteric arteries. The physiological saline solution is bicarbonate balanced and pH with 5% CO2, 20% O2 and nitrogen balanced, back temperature is...
25 February 2022 8,060 0 View
ABSTRACT A master's thesis on the topic: "Optimization of parameters of gas turbine working in climatic conditions of the Republic of Iraq" Year of defense: 2017. Specialization: 13.04.01 «Heat...
15 January 2022 712 11 View
I am trying to use 300 um hippocampal sections for immunohistochemistry but the antibodies have not penetrated deep enough (
26 October 2021 1,283 0 View
I´m using Leica VT1000 vibratome for slicing rat´s cerebellum for patch clamp experiments. Which parameters are better to use? Thanks
25 October 2021 9,576 1 View
Grid-walking test refers to the method to assess the locomotion accuracy of a rat/mouse as described in PMID: 22142899. It's an ardous work to stop the video recordings again and again to count...
19 October 2021 5,045 1 View
Hello , Did anyone here used a specific anti-rat NK cell antibody which works in rat paraffin sections (FFPE) ? I have found that ANK44 (Abcam) and others which are reactive to NK cells, a T...
15 October 2021 5,167 0 View
I am asking about a detailed protocol.
13 October 2021 4,315 4 View
I'm recently working on anthelmintic activity in vivo on rats for which I need to induce helmenthiasis in rats can anyone suggest me that how should I grow parasite
04 October 2021 2,792 3 View
Best module for breast cancer induction.
29 September 2021 4,205 3 View
What is the best approach to induction of anemia in experimental rats?
16 September 2021 4,116 4 View
I couldn't find any anti-rat CD20 which has been tested for in vivo B cell depletion in rats. Could any anti-rat CD20 or CD-19 ab work for B cell depletion in rats?
16 September 2021 4,773 2 View
Hi, I am interested in chemogenetically manipulating GABA cells and came across 2 genetic rat lines: VGat-IRES-cre ad Gad1-cre rat lines. I was wondering what the difference between these two...
09 September 2021 8,559 1 View
Hello! I am having problems in the cultivation of brain microvessel cells. I isolated cells from adult rats. In cultivation, I used 4 μg / ml puromycin for 2 days. (1 image) I have 3 problems:...
08 September 2021 9,414 1 View
I have done beta gal assay in mcf10 A cell line. I am not familiar with the result. Would you please let me the know that whether the uploaded cell image are showing senescence or not?
06 September 2021 732 3 View
I am planning to perform in vitro electrophysiology experiments on rats model. Is it possible to store whole rat brain for invitro electrophysiology experiments or one have to perform the...
04 September 2021 6,034 2 View
Hello, I have to administer antiplatet drugs, such as aspirin, clopidogrel, prasugrel, ticagrelor to rats and I am thinking of oral gavage as the preferred route. Which you consider to be the...
03 September 2021 4,286 2 View
My recent results indicate a decrease in the expression of beta-catenin in rat cardiomyocytes in response to a specific drug and in-silico model of binding energies between the said drug and...
30 August 2021 7,898 1 View