The common name for the genus Rattus. | Review and cite RATS protocol, troubleshooting and other methodology information | Contact experts in RATS to get answers
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Questions related to Rats
I have Started Working on Diabetic Rats and I need to assess the activity of below mentioned Enzymatic activity Hexokinase Aldolase Phosphoglucoisomerase Glucose-6-phosphatase Fructose-1,...
07 April 2021 6,477 3 View
Dear colleagues in a study which aims to test the effect of a medicinal plant on obesity, I want to know if there is a protocol for rapidly inducing obesity in rats.
03 April 2021 6,697 7 View
Hi all, Recently, I am trying to measure LTP signal, but I cannot detect the response. I sliced the brain horizontally with vibratome. For slice, i used ice-chilled aCSF (0.5 mM, CaCl2), and the...
25 March 2021 6,767 3 View
I want to know how many isolated rat pulmonary artery rings, I can get from a rat, for use in the organ bath. So how long is a rat's main pulmonery artery. Thank you.
23 March 2021 2,906 1 View
For my experiment, we are examing the effects of estrous stage on heroin withdrawal enhanced fear learning in rats, and for several of the test days we would like to acquire the vaginal cells by...
22 March 2021 9,808 1 View
Toxicology studies say, Ethanolic extract of A.calamus even at a higher dose(3gm/kg) there is no toxicity which is done on adult rats. How to fix therapeutic dose for infant rats.
20 March 2021 8,506 6 View
We would like to categorize anxiety-like and locomotor activity from rats tested in the elevated plus maze and in open field, for correlation analysis with gut microbiota. Thus, we are looking for...
04 March 2021 7,063 4 View
Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...
23 February 2021 800 1 View
I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...
16 February 2021 3,035 2 View
My thesis involves experimental study with Wistar puppies. I am dividing the puppies equally in relation to the groups. However I only have 4 mothers and I will use all of their puppies. There is...
26 January 2021 7,179 3 View
I am working on the determination of tulathromycin in rat faeces using QE-focus (Thermo Fisher)(Waters BEH C18, 2.1x100mm, 1.7um)(0.1%FA+H2O, 0.1%FA+ACN) . I encountered two tricky problems....
03 January 2021 2,964 5 View
Hello, I'm using a Rabbit Glucagon antibody from Abcam to stain human primary islets and iPSC derived islets for Glucagon along with other pancreatic cell markers such as PdX1 and C-peptide. The...
29 December 2020 1,436 0 View
Dear, We developed a microcarrier that was pre-seeded hUC-MSCs to repair a rat bone defect. However, we didn't use GFP or Luciferin to track cell survival. If there's any way to identify the...
16 December 2020 6,230 3 View
Is anyone able to tell me why my anaesthetized rats often do a 'double breath', or diaphragmatic breath, like a hiccup (see video). This often begins after 1-2 hours under anesthesia, and can...
07 December 2020 2,678 5 View
I am looking for an anti-rat CLEC-2 antibody for IHC in rat lungs. Has anyone come across one? There are a few mouse ones but the data sheets don't say they cross react with rat, and the ones that...
07 December 2020 3,680 0 View
I have 1L of hybridoma supernatant without serum from a rat monoclonal antibody. I would like to purify it using a binding affinity column for rat kappa chain. Could you please tell me where I...
30 November 2020 3,471 3 View
The antibodies to human sexual hormones are much easier to get than antibodies to rat sexual hormones. So, the question is raised how correct is application of antibodies to human sexual hormones...
21 November 2020 5,254 1 View
Hi, I am interested to know the DNA sequence of the TF corrisponding to the DNA binding domain. Do you know any tool developed recently that can help?
15 November 2020 9,694 6 View
The rats are perfused with PBS and the brain sections are 18 microns thick and fixed on slides.
10 November 2020 956 3 View
Dear colleagues, I would like to ask you if anyone has experience with the fixation of whole rat organs (heart, lungs, liver, kidneys, brain, testes) in 10% buffered formalin. Thank you, Roman
29 October 2020 3,344 3 View
How should I measure motility, what is the process? Should I put it in the incubator (37 C) immediately after sacrification? Thank you from now.
29 October 2020 422 4 View
how can i collect many blood sample from the rat for pharmacokinetic study without killing the rat??
29 October 2020 2,856 8 View
I'm working on a mesenchymal stem cell culture isolated from rat bone marrow and I want to yield a specific MSCs count from passage 3 . I want to know the yield of cells in passage 3 to know how...
28 October 2020 544 1 View
We need to collect CSF samples repeatedly from the 4th or lateral ventricle of rats. Does anyone know of a protocol for implanting a cannula to be able to do this? Or even a protocol for this...
27 October 2020 5,975 3 View