Can rats orally consume ketamine which is added in their drinking water?

More Ege Kingir's questions See All

How to prepare miRNA-Seq library prep pipeline?

Hey everyone, I am trying to do a miRNA-Seq experiment, but as you all know, before doing so I need to prepare a library and when reading up on it and asking people from around the field I still...

28 July 2018 4,336 5 View

Is it possible to extract and purify miRNAs from frozen whole blood samples?

Hello everyone, I have been trying to find a reliable and reproducible method for extracting miRNAs from whole blood. I am aware there are PAXgene tubes, however the blood samples are collected...

25 April 2018 6,706 3 View

What are the applications of NGS systems that are useful for miRNA-Seq?

Hello everyone, For my project I need to do a comprehensive miRNA-Seq experiment and at the moment our collaborators only have a MiSeq System which as far as I know is a targeted sequencing...

26 October 2017 3,051 4 View

Affordable and Reliable RNA Sequencing Core Facility?

Hello everyone, For my research I need to do some RNA-Seq experiments in order to generate a miRNA profile from scratch from blood serum samples. However, we have a specific budget for this...

23 October 2017 585 3 View

Quantile Normalization and Data Processing

Hello everybody, I am fairly new to microarray expression analyses, therefore I am trying to reproduce data from raw data provided from a different research experiment. In the paper it...

10 August 2017 6,683 3 View

Why are my Ct values are vastly different from each other in qPCR? Also any tips on how to generate the graphs related to qPCR manually?

Hey everybody, I did a qPCR and gained results, however in some of my samples, one of my triplicates has a much higher Ct value than the others. This is quite frequent but only just one sample is...

15 August 2016 2,032 4 View

How to do cDNA synthesis without a kit?

Hello everyone, I have been having issues with the cDNA synthesis that I have been doing recently and wanted to check if there is anything that I can do to improve it as my results have not been...

03 August 2016 3,376 5 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

Can any tell me or suggest any research article or review about the concentration of peptide dissolve in system water and given to Adult Zebrafish?

Hello, everyone! I have chemically synthesized antioxidant peptide isolated from the plant. I have to validate its bioactivities in Adult zebrafish. I have to administer this peptide to zebra...

01 February 2021 5,809 3 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Jim Macdonall

Ege,

I do not know the answer. But rats can be induced to drink sapid solutions without using sugar. See

Patterns of ethanol consumption as a function of the schedule of ethanol access.

Marcucella H, Munro I, MacDonall JS.J Pharmacol Exp Ther. 1984 Sep;230(3):658-64.PMID: 6470974

Jim

Ege Kingir

Jim Macdonall Thank you very much for your answer sir! However as far as I know, low concentrations of ethanol is not a bitter drink for rats. Ketamine, on the other hand, is considered as bitter, so it is said that rats don't drink it just inside the drinking water. Is there any alternative way for bitter tasting drugs that you know of?

You're welcome.

The method in my article has been used to increase consumption of quinine, a bitter substance. See my reference list for articles.