The common name for the genus Rattus. | Review and cite RATS protocol, troubleshooting and other methodology information | Contact experts in RATS to get answers
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Questions related to Rats
4-nitroquinolone oxide (4NQO) is approved as a carcinogen/strong mutagen in experimental animals, especially on rat. I am looking for the mechanism of this carcinogen on rat tongue tissue, why not...
19 April 2019 4,684 1 View
Hi all, please help me with this question. Thanks a lot in advance!
19 April 2019 4,453 3 View
I'm currently studying perinatal opioid exposure in rats. We've been using morphine pellets, but are considering switching to osmotic minipumps. However, we want to ensure that our dosing is as...
19 April 2019 3,167 2 View
We are attempting to develop a protocol for cryostat section on adult male and female mice brains. I was curious to see if anyone had a protocol that listed approximately how deep into the brain...
16 April 2019 5,608 3 View
I will be collecting blood samples from rats (in EDTA-coated tubes) and centrifuging to extract the plasma. My question is: what type of tube should I be using to aliquot the plasma for long-term...
08 April 2019 4,924 3 View
I am testing some compounds for wound healing in rats (rodent) and want to further extend the research for the drug development for wound healing in animals/human. So, please tell me the different...
31 March 2019 8,370 3 View
Hi, I want to use potassium channel (BKca) opener as a standard for in vivo evaluation of my compound in rats for anti-hypertensive activity.
27 March 2019 7,642 2 View
Dear all, does anyone know the protocol for how to slice a cortex (rat). The major doubt I am having is, how to position the cortex for IHC?
18 March 2019 4,041 3 View
Dear all, I am using SB431542, a Smad 2/3 inhibitor to treat dermal fibroblasts from rat. Beside proliferation, I want to test the effect of SB431542 on the function of fibroblasts. Is...
14 March 2019 9,744 2 View
Different protocols to measure anhedonia in rats by 'sucrose preference test'. Not an standard procedure available.
13 March 2019 1,237 6 View
Hello, I'm working with a fungicide, I'm trying to see its effect on rats. My problem now is with the dose, in articles I see that they take the LD50 and they divide in 100 and they take the...
13 March 2019 4,231 3 View
Hello, I'm working with rats in a toxicity test, I'm testing the effect of a fungicide and I would like to know the minimal number of rats I need to start my toxicological studies? Test rats and...
11 March 2019 2,020 12 View
I'm trying to perform a vaginal smear from female rat but I couldn't get any vaginal secretions, I insert 100ul saline and withdraw it a gain from the vagina but no cells are found in the smear
06 March 2019 4,610 8 View
Had anyone checked for miR-133 or miR-1 expression in Schwann cells before? I'm having inconsistent results (Taqman qPCR) with different generations of rat Schwann cell cultures here (pre n+10 vs...
01 March 2019 8,444 0 View
I am in doubt as to the best volume for intranasal instillation in rats, what volume ensures the best distribution of the particles?
21 February 2019 3,031 1 View
I am researching on a chemotherapeutic agent for gliomas which i intend to deliver using nanotechnology.
16 February 2019 9,683 4 View
Dear all, I am trying to find the best way to measure HbA1c in rats from our current project. Most commercially available kits for rat HbA1c won't work since they either require whole blood,...
13 February 2019 7,301 1 View
Trying to detect MMP3 bands and in need of a positive control. I ran rat lung lysate and looks like is showing some bands at MMP3 and MMP13 molecular weight. Does somebody knows is it could serve...
06 February 2019 6,325 1 View
Why do we prefer male rats instead of female rats in obesity-related studies? Is there an important difference in terms of body weight, blood lipids, catecholamines etc.
28 January 2019 6,796 9 View
Hello everybody I am at the first steps in Alzheimer's research. I just want to know the most routine and acceptable protocol for Alzheimer induction in rat.
15 January 2019 7,690 2 View
Does anyone know of recordings of 2D MDCK / BMEC permeability or 3D rat brain perfusion measurements of temozolomide permeability? Thanks!
14 January 2019 2,199 14 View
Lipid level include cholesterol, HDL, triglyceride and LDL
09 January 2019 1,752 3 View
We have a human OSKM gene cosntruct that we want to use to reprogram mouse and rat fibroblasts as well as human cells.
30 December 2018 1,685 2 View
Hi Researchers, i want to induce insulin resistance/T2D in rats and i found that, i can give the animals 10% fructose water to drink for two weeks before giving low dose of streptozotocin. Can...
26 December 2018 10,024 5 View