The common name for the genus Rattus. | Review and cite RATS protocol, troubleshooting and other methodology information | Contact experts in RATS to get answers
7,051 views 1,078 posts
Questions related to Rats
The rats are perfused with PBS and the brain sections are 18 microns thick and fixed on slides.
10 November 2020 1,113 3 View
Dear colleagues, I would like to ask you if anyone has experience with the fixation of whole rat organs (heart, lungs, liver, kidneys, brain, testes) in 10% buffered formalin. Thank you, Roman
29 October 2020 3,521 3 View
How should I measure motility, what is the process? Should I put it in the incubator (37 C) immediately after sacrification? Thank you from now.
29 October 2020 594 4 View
how can i collect many blood sample from the rat for pharmacokinetic study without killing the rat??
29 October 2020 3,039 8 View
I'm working on a mesenchymal stem cell culture isolated from rat bone marrow and I want to yield a specific MSCs count from passage 3 . I want to know the yield of cells in passage 3 to know how...
28 October 2020 713 1 View
We need to collect CSF samples repeatedly from the 4th or lateral ventricle of rats. Does anyone know of a protocol for implanting a cannula to be able to do this? Or even a protocol for this...
27 October 2020 6,299 3 View
I am looking to study neurons of the bed nucleus of the stria terminalis. I have seen reports using coronal sections. Others present the distinct nuclei in transverse sections. What is the best...
23 October 2020 3,645 1 View
Hello every one, i´ve been working with rats for a few years but now i'm wondering wether there is a way to calculate the BMI or the adipose tissue in these animals. Also, i've been searching...
23 October 2020 3,117 5 View
I want to know that in which quantity if at all needed we should add sodium sulphate to cattle feed, so that we can see increase in milk production and reduction in methane production. Also in how...
22 October 2020 4,835 4 View
Our lab is trying to establish a BT-474 xenograft model. We have not been able to get the tumors to grow past 50-100mm3. We inject 1x107 cells in a 50:50 matrigel suspension. We used nude mice (5...
21 October 2020 2,917 4 View
Good day house. Please I intend to start exposure and treatment in Rats and Mice on the first day of pregnancy. So how do I determine the first day of pregnancy which is always designated as...
16 October 2020 7,715 4 View
I want to measure the levels of lipopolysaccharide (LPS) in rat serum and if possible, rat brain tissue samples. There are a lot of kits out there that can measure LPS in serum but I wasn't sure...
11 October 2020 396 0 View
I have a problem about rat anesthesia, the situation is that I have Zoletil 50 1mg/ml + xylazine hydrochloride 23.32mg/ml solution that I prepare at the same time from the same source (from the...
08 October 2020 9,504 7 View
Hello everyone, I need help from you with my CD31+Dystrophin analysis. The question is: How to analyze the Capillary Density and Capillary-to-Fibre-Ration in skeletal muscle? Any tips when you...
08 October 2020 905 0 View
I am performing LM on whole rat brains with a specific focus on the hippocampus and cannot find which information on which clearing technique is better.
06 October 2020 1,650 1 View
Hello sir. I am currently a master's student, and I am conducting pharmacokinetic studies in rats on the drug Posaconazole. However, I have encountered some problems recently. That is, the...
05 October 2020 7,416 1 View
I need to calculate the concentration of viral antigens or viral load in a given amount of mucus in an adult experimentally infected with RSV. The only comparable test I have come across in the...
02 October 2020 9,467 1 View
Is continuous glucose monitoring, comparable to what is existing in humans, available for mice and/or rats without large surgery ? thank you !
22 September 2020 6,315 2 View
Does anyone have experience in Hymenolepis infestation in rats? I have given them praziquantel for about 3 terms as the eggs were still found in the feces when it was evaluated 5 days after the...
16 September 2020 1,085 1 View
Dear all, I was thinking about sorting single-cell suspensions of wild-type mouse hippocampal neurons into glutamatergic and gabaergic neurons. As time is a critical factor, I was wondering...
16 September 2020 8,927 0 View
As we know, rats are very resistant to rise in blood glucose and lipids via high-fat diet. so, what is the lowest effective percentage of fat in the diet of them for the induction of type 2...
15 September 2020 2,486 3 View
I can fathom the idea of PK being different b/w dogs and rodents, but why would I be seeing a significantly different PK profile for the same drug tested on rats and mice?
11 September 2020 9,005 5 View
When the rats were induced diabetes with the administration of Streptozotocin Section from pancreatic islets and acini shows cytoplasmic vacuolation. Islets are small in size with reduced in the...
10 September 2020 9,655 2 View
Tissue processing techniques in particular..
07 September 2020 6,015 3 View