The common name for the genus Rattus. | Review and cite RATS protocol, troubleshooting and other methodology information | Contact experts in RATS to get answers
2,384 views 615 posts
Questions related to Rats
I`m studing melosuppression and i want to count bone marrow cells
27 September 2019 5,426 3 View
Hello everyone i read number of articles but everyonechoosed diffirent dossage and route of administration but which is the best dossage creating testicular toxicity in rats and route of...
26 September 2019 7,273 3 View
I would like to induce inflammation in rats using carrageenan, but I realized that there are mainly 3 types of carrageenan kappa, iota , lambda carrageenan and I found many products available in...
18 September 2019 1,619 1 View
I am looking for an animal model espically rats that simulate metabolic syndrome in human
07 September 2019 843 2 View
Good afternoon everybody. Could you tell me whether this dotplot is OK for rat leukocytes or not? Glanulocytes seem to move to the left. They are not located above monocytes. Thank you in advance.
13 August 2019 6,381 3 View
What is the best chemical in inducing PCO? is Letrozole or Estradiol-Valerate EV? How can I get non-time consuming method in PCO induction? and what are the doses? Regards;
02 August 2019 4,705 11 View
I need a method to evaluate chromatin of rat sperm.
31 July 2019 3,852 3 View
Hello there, I'm trying current-clamp recording from rat hypothalamic slices. In the literature, the hypothalamic neurons should fire spontaneously, but almost all of my neurons go deep (-80~-90...
19 July 2019 9,359 12 View
I am using the BB1 receptor antagonist PD168368 at 10uM as has been shown in the literature, but am having trouble adequately inhibiting activity through this receptor in the basolateral amygdala...
17 July 2019 3,096 0 View
Hi, I want to isolate and culture macrophages from rats (different sites like bone marrow, peritoneal fluid etc.) Most of the reported literature says to use the supernatant from mouse fibroblast...
12 July 2019 7,405 3 View
Hi all, I have found loads of paper to fix rat testes but plenty of protocol variations. I have started to fix some testes in NBF 10% for 24h but it doesn't look enough fixed. Do someone...
08 July 2019 4,835 1 View
Hellos. Im seeking advice on what would be the best model of T2D in rats given the following constraints: 1) I do not prefer leptin or leptin receptor deficient models since these mutations...
05 July 2019 5,702 3 View
The ones provided by the companies go only up to 12 weeks.
27 June 2019 9,725 1 View
Please anyone help me to understand the result of continuous EEG recording from rat in different stress condition. Whenever I calculate the absolute band power of specific band I always get the...
22 June 2019 9,241 2 View
We have a monoclonal antibody raised in rats and we would like to sequence it. Can someone advise on the optimal primers we could use.
21 June 2019 5,283 2 View
I give C. rodentium to mice by oral gavage. 10Exp9 CFU dissolved in 500 ul PBS to one mice. All mice will survive after oral gavege. But the infection load (from feces) varies from 10Exp8 CFU/g to...
21 June 2019 8,857 0 View
Hi Does anyone have a detailed protocol for bronchoalveolar lavage (BALF) in rats? So far my LIVE/DEAD cell ratio in the BALF is very low (20%/80%). Also can anyone share a Wright-Giemsa...
20 June 2019 3,728 1 View
Hi, I am doing WB with heart tissues (Rat) these days and having a problem of high background. We have no issue of getting high background for WB done with cells or other tissues, the protocol is...
12 June 2019 2,798 3 View
Observed a relative increase in both parent and its metabolite exposures in body fluids following intake of parent form in rats. The increase was observed in highest test dose compared to lowest dose.
24 May 2019 3,002 3 View
Hi, Does anyone know how to isolate the hematopoietic stem cells from rat bone marrow ?
23 May 2019 7,853 3 View
If I would like to study the effect of a small molecule compound in animal, say rat. Do I need to conduct a dose-response study with 4 to 5 different doses in rats? Usually people find the maximum...
14 May 2019 9,296 3 View
I am starting a project with melatonin supplementation and would like to make a stock solution for dilution when giving the melatonin to rats; I suppose if I dilute the melatonin in alcohol and...
08 May 2019 6,931 2 View
We observed lower-limbphagia from phalanx to knee in transection models of spinal cord injured rats. How can we manage and prevent it ?
06 May 2019 3,498 5 View
I am going to be inducing pluripotency in adult rat dermal fibroblasts and adult rat bone marrow cells, and eventually the same with adult human peripheral blood CD34+ monocytes. Can anyone...
01 May 2019 8,365 5 View