The common name for the genus Rattus. | Review and cite RATS protocol, troubleshooting and other methodology information | Contact experts in RATS to get answers
7,052 views 1,078 posts
Questions related to Rats
I have Started Working on Diabetic Rats and I need to assess the activity of below mentioned Enzymatic activity Hexokinase Aldolase Phosphoglucoisomerase Glucose-6-phosphatase Fructose-1,...
07 April 2021 6,608 3 View
I mean a coating with LPS on glass coverslips to perform immunocytochemistry through cell adhesion upon LPS. I can't find anything in literature. I can only find LPS coating on plastic wells for...
05 April 2021 2,217 0 View
Dear colleagues in a study which aims to test the effect of a medicinal plant on obesity, I want to know if there is a protocol for rapidly inducing obesity in rats.
03 April 2021 6,830 7 View
Hi. I had done a non probability survey to collect information on retirment planning among workers about to retire and those who had already retired. I now want to compare the two populations...
29 March 2021 5,105 3 View
Hey everyone, I am working with a pancreatic cancer cell line which forms tumors in the pancreas in the orthotopic mice model and the same cells form zero colonies in the soft agar assay. I was...
29 March 2021 6,380 0 View
Hi all, Recently, I am trying to measure LTP signal, but I cannot detect the response. I sliced the brain horizontally with vibratome. For slice, i used ice-chilled aCSF (0.5 mM, CaCl2), and the...
25 March 2021 6,923 3 View
I want to know how many isolated rat pulmonary artery rings, I can get from a rat, for use in the organ bath. So how long is a rat's main pulmonery artery. Thank you.
23 March 2021 3,039 1 View
For my experiment, we are examing the effects of estrous stage on heroin withdrawal enhanced fear learning in rats, and for several of the test days we would like to acquire the vaginal cells by...
22 March 2021 9,945 1 View
Toxicology studies say, Ethanolic extract of A.calamus even at a higher dose(3gm/kg) there is no toxicity which is done on adult rats. How to fix therapeutic dose for infant rats.
20 March 2021 8,642 6 View
I am currently trying to perform co-culture of Astrocyte/Schwann cell (primary culture), for Immunostaining I have to use antibodies specifically recognize each cell type, to do so I have been...
11 March 2021 3,945 0 View
We would like to categorize anxiety-like and locomotor activity from rats tested in the elevated plus maze and in open field, for correlation analysis with gut microbiota. Thus, we are looking for...
04 March 2021 7,221 4 View
Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...
23 February 2021 1,085 1 View
I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...
16 February 2021 3,262 2 View
My thesis involves experimental study with Wistar puppies. I am dividing the puppies equally in relation to the groups. However I only have 4 mothers and I will use all of their puppies. There is...
26 January 2021 7,409 3 View
I am working on the determination of tulathromycin in rat faeces using QE-focus (Thermo Fisher)(Waters BEH C18, 2.1x100mm, 1.7um)(0.1%FA+H2O, 0.1%FA+ACN) . I encountered two tricky problems....
03 January 2021 3,154 5 View
Hello, I'm using a Rabbit Glucagon antibody from Abcam to stain human primary islets and iPSC derived islets for Glucagon along with other pancreatic cell markers such as PdX1 and C-peptide. The...
29 December 2020 1,591 0 View
When multiple vendors supply equipment, who determines what information is available to the owner and the BACnet operator workstation? Is all the information available to the BACnet operator...
22 December 2020 7,195 0 View
Dear, We developed a microcarrier that was pre-seeded hUC-MSCs to repair a rat bone defect. However, we didn't use GFP or Luciferin to track cell survival. If there's any way to identify the...
16 December 2020 6,493 3 View
Is anyone able to tell me why my anaesthetized rats often do a 'double breath', or diaphragmatic breath, like a hiccup (see video). This often begins after 1-2 hours under anesthesia, and can...
07 December 2020 2,930 5 View
I am looking for an anti-rat CLEC-2 antibody for IHC in rat lungs. Has anyone come across one? There are a few mouse ones but the data sheets don't say they cross react with rat, and the ones that...
07 December 2020 3,860 0 View
I have 1L of hybridoma supernatant without serum from a rat monoclonal antibody. I would like to purify it using a binding affinity column for rat kappa chain. Could you please tell me where I...
30 November 2020 3,646 3 View
The antibodies to human sexual hormones are much easier to get than antibodies to rat sexual hormones. So, the question is raised how correct is application of antibodies to human sexual hormones...
21 November 2020 5,525 1 View
Hi, I am interested to know the DNA sequence of the TF corrisponding to the DNA binding domain. Do you know any tool developed recently that can help?
15 November 2020 9,844 6 View
I recently switched lot of a primary antibody used in a competitive RIA. I have performed a titration experiment and the new antibody now must be used a a 1:1M dilution to give the same maximum...
11 November 2020 5,160 0 View