Is there any RNA marker known for exosome that can be used as internal control in qRT-PCR for exosomal RNA
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
While working on caisson foundation, I applied static vertical load
25 July 2024 9,357 1 View
We have seen in the literature that Ang-1 can be considered as one of the markers. We need information about few more markers.
01 June 2024 9,650 1 View
Notch 3 can be considered as marker for stable blood vessel formation?
01 June 2024 9,788 0 View
Both angiogenesis and arteriogenesis are needed to make stable blood vessel formation. Ang-1 is one of the markers for stable blood vessel formation. Can you please suggest names of few more...
01 June 2024 1,912 1 View
Globally what are the Research opportunities in stabilizing overburden dump slopes in opencast mining?
16 May 2024 5,334 1 View
Conceptual Designing of aircraft provides a major role in designing of aircraft. Let's consider the weight estimation in it. For electric aircrafts its fuel variation is " zero ". Hence W|f is...
18 March 2024 946 3 View
I conducted X-ray diffraction (XRD) investigation on my chitosan sample and need assistance interpreting its diffractogram. If possible, could someone share the chitosan JCPDS card number with me?...
01 March 2024 2,080 4 View
PTFE: Polytetrefluoroethylene or teflon and PPL: Polypropiolactone
14 February 2024 6,223 0 View
I am able to run PMG and dfe but not mg in Stata. they are showing an error and saying __ec is listed as a predictor. But I had dropped these variables including est variables before running MG....
12 January 2024 5,646 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?
05 August 2024 10,034 1 View