Is there any RNA marker known for exosome that can be used as internal control in qRT-PCR for exosomal RNA
For electrochemical sensors, the electroactive surface area (ESA) show higher value for the working material as compared to bare (reported in several papers). My bare electrode is FTO and working...
17 January 2021 7,220 3 View
I am new to PCR. We are working on PCR based amplification of targeted DNA sequence of Plasmodium Falciparum. For that, We are targeting the Ring stage of the parasite. I want to know on which...
15 December 2020 6,954 3 View
The current study Digital Marketing in Indian and its Impact on Consumer behavior in post and Pre-covid-19 in Agra a district of the state utttar pradesh India has to be taken up . How should I go...
06 December 2020 5,614 4 View
I have tried using PowerSoil Pro kit from Qiagen with slight modifications- incubating at 65 degree for 10 mins after adding solution CD1, increasing the number of washes with CD5 to 3 times and...
27 October 2020 7,782 3 View
I came to know that there is no oilglands in sandalwood and it is a byproduct during transformation of sapwood to heartwood. Is it true?
04 October 2020 8,216 2 View
Hello all, I need a small help for downloading free climate data sets for annual mean temperature, rainfall, flood and drought for period of 30 years of Bihar, India. Please suggest me some...
04 October 2020 1,939 19 View
Hi, Can someone share the CIF file (XRD analysis) for SFCA, SFCA I, SFCAII, CF, Ca-Magnetite. Can't find these in COD Thanks, Moni
25 August 2020 7,377 2 View
I have provided the images reported in journal papers. Which technique in TSL OIM Analysis 7 is used to identify the planes and directions in the EBSD map? How is it colour coded with the pole...
03 August 2020 8,078 1 View
Dear Sir/Madam, I want to make Private the loaded research papers in the Research Gate . How can I do this? with regards Sincerely H N Sinha
23 July 2020 4,520 4 View
If the ultra-low carbon steel is held at high isothermal temperatures will it be possible to form pearlite? How can the formation of pearlite be confirmed other than morphological confirmation?
14 July 2020 9,251 4 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...
28 February 2021 6,560 14 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View