Any way to separate out fluorescent channels from a composite image?

Hi there, I've been using the EVOS Auto II microscope for some time now and usually I will save the transmitted light/bright field image and the images from fluorescent channels as separate...

15 February 2021 546 3 View

Cells have high and low confluency in same flask?

I'm currently growing OVCAR3 and OVCAR8 cells in T225 flasks. I returned to the lab a couple of months ago after being away for nearly half a year due to COVID-19, and since I've returned I've had...

23 October 2020 3,683 4 View

How do I perform procrustes analysis after Bayesian PCA?

I'm testing different methods of calculating missing landmarks for geomorphic morphometric analysis of rodent footprints. After an initial literature review, I came across Bayesian PCA (BPCA) and...

02 March 2020 6,756 3 View

CD45 stain disappearing after intracellular staining (image cytometry)?

I'm having issues with the stability of my CD45 stain conjugated with APC. I'm staining PBMCs for Circulating tumour cells. If I do not do the intraceulluar (CK) staining and simply CD45 stain...

02 January 2020 9,609 2 View

Tips for extracting DNA from clotted, frozen avian red blood cells?

I have clotted, frozen avian red blood cells that I am attempting to extract DNA from (for eventual micro satellite sequencing). I am using the Qiagen DNeasy Blood and Tissue Kit. The protocol was...

18 March 2019 8,656 3 View

What's the best way to contact you?

I'm working with some folks involved in the Herstory Project - I think they would be interested. [email protected] Thanks.

09 December 2018 3,461 3 View

I'm working with some folks involved in the Herstory Project - I think they would be interested. What's the best way to contact you?

[email protected] Thanks.

09 December 2018 4,784 2 View

Does anyone know this cell phenotype?

25 July 2018 744 1 View

Isolating tumor RNA in a sterile manner

Has anyone isolated tumor RNA from cell lines for use in cell culture? In all the papers I've read I can see no mention of isolating the RNA in any more sterile manner than is required for any RNA...

20 February 2017 8,530 3 View

Triple transfection mechanism : does GFP testifies of efficient transfection when expressed ?

Hi everyone, Pardon me if the question sounds a bit stupid but I just would like to be sure to understand the triple transfection mechanism. I'm performing triple transfection for AAV2 production...

23 September 2015 2,726 12 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View