How to validate image labeling quality within dataset?

Hello, it is about creating a dataset for semantic segmentation with three classes. The problem is that one class dominates with >90% and one class is

18 February 2021 4,254 5 View

Are there any DNA extraction kit from resins?

Hi everyone, I'm looking for a DNA extraction kit or some protocols to obtain the total DNA from resin samples. For this purpose, some studies suggest the use of plant extraction protocol of...

25 October 2020 3,898 3 View

How to calculate the effect size of a mixed models anova (from existing research papers)?

Hi all I'm comparing some research papers for my university. The most studies use a mixed model ANOVA design, but did not specify the effect sizes. 1. Is it possible to (re)calculate the effects...

23 October 2020 5,013 3 View

Antibody : loss of binding at high concentration (beyond saturation) Why?

Hi everyone, It is commonly observed that some antibodies presents a binding saturation curve that goes down at high concentration (at concentration beyond saturation). Does anybody have a good...

20 August 2020 778 5 View

TRP1 deficient yeast strain not growing on 5FAA containing medium?

05 August 2020 3,010 5 View

What would be the ideal number of epoch for my Neural Network?

Hello, If I am training my Neural Network with training data comprised of 908 batches, as the rule of thumb, would 20 epochs for training be sufficient? If 20 epochs are not sufficient, does...

26 March 2020 2,291 6 View

How important is it to set seed in experiments in computer science?

Hello, If I am going to submit my work to a CS conference, and also make my Python code available publicly, is it absolutely necessary that I set seed in my Python code? I ran an experiment, but...

07 March 2020 1,832 3 View

How to process ARC dataset with GTP2 Double Heads Model?

Hello, I am interested in processing the ARC dataset (http://nlpprogress.com/english/question_answering.html) with the GPT2 double heads model neural network. The dataset (tab delimited) is...

22 November 2019 3,796 1 View

How to distinguish "elementary English" from "advanced English"?

Hello, When we take a look at English test prep books that are specially designed for ESL learners, we often see in their title that the book is for "learners at elementary level", "learners at...

15 November 2019 5,393 1 View

Can someone please guide me to a software that I can use to create time lapse models for the marine environment?

I am working on a concept related to the formation of coral reefs and I need to demonstrate the concept in the form of a visual model that changes over time. I am fairly new to this area of...

16 November 2018 9,276 4 View

RWP1 growing in RPMI medium?

Hello everyone, Has anyone try to culture the normal prostate RWPE-1 cell line in RPMI medium supplemented with foetal bovine serum? There's some articles available but neither describe details...

08 February 2021 6,455 1 View

Is there any possibility to buy specific drug resistant human and mouse tissue array from company or other research organisations?

I need some specific cancer tissue samples' slides for IHC which is resistant and sensitive to specific cancer drugs, especially for prostate, lung, and breast cancer. Is it possible to buy or...

27 November 2020 731 2 View

What are the morphological changes of PC3 cells during autophagy and/or apoptosis?

I was performing wound healing assay seeding 300,000cells/well in 6 well plates with 2mL RPMI medium. At 0hr after intervention, the cells looked like the first picture. At 24hr after...

18 November 2020 9,671 2 View

How many samples/measurements do I need for certain a sensitivity & specificity?

I'm taking some measurements using Raman spectroscopy of healthy pig eye samples using a new excitation wavelength and my supervisor said to choose a sample size to allow for spec/sens of 85%....

01 November 2020 8,611 6 View

Glucose dehydrogenase interference with glucokinase assay?

Hello all, I'm currently working with undergraduate student-researchers (there are no graduate students at my university) to purify and characterize the drug targetability of glucokinase from pig...

25 October 2020 3,553 7 View

What statistical test should I use?

Hi, I am trying to choose the appropriate statistical test to evaluate cells (drug vs control) over 4 different time points (inc. 0hr). Specifically I was testing prostate cells treated with a...

12 October 2020 2,870 19 View

Should antiandrogens and / or TMPRSS2 protease inhibitors be given in adult patients without prostate cancer and also in women? Only in men? Never ?

As we know, not long ago the WHO named Wuhann Pneumonia (SARS-COV 2): Covid-19 (from Coronavirus Disease 2019), which has become a serious pandemic and health emergency with multiple pathological...

21 September 2020 4,618 13 View

Which wedium do we use for prostate cancer LNCaP cell line?

Dear Scientists, I am a Master student and I am doing an anti-cancer effect on LNCaP cell line. I have not had any experience with this cell line before. I found on ATCC and some papers, they...

14 September 2020 3,375 6 View

What is the best way to extract DNA from Pig's fecal sample?

Dear Colleagues, As we know the DNA extraction steps of animal fecal sample is bit different than human fecal sample. For instance, we generally use QIAamp DNA Stool Mini Kit to extract DNA as a...

09 September 2020 1,927 4 View

PC-3 cells: what does a neuron like/threadlike morphology indicate about the cell's condition?

I was performing wound healing assays with PC-3 cells (cultured in RPMI+10%FBS; 500,000 cells/well in 6 well plate). I seeded them for 24 hours, then applied the scratch with a 100uL pipette tip....

03 September 2020 7,664 4 View