Has anyone tried to fluorescently label a Histagged protein bound to a Ni-NTA column?

We have a protein that is soluble when bound to the column but precipitates at the high protein concentrations and high pHs required for all of the coupling reactions. We were thinking of labeling...

25 August 2019 5,840 3 View

Can hearing stories support individuals and communities to create a new perception, a way of seeing that did not exist before hearing the story told?

As oral storyteller and researcher, my approach is heuristic; it is my experience that folktales and life stories offer encouragement, via the characters/protagonist journey, that fosters sound...

12 November 2017 4,938 2 View

Are there any books or journals I should read that may provide information about how long an intervention should last in a quantitative study?

I am conducting a quantitative study using a quasi-experimental design. I need help locating information about required sample size and how long the intervention should last. Are there any...

12 March 2015 4,218 3 View

Leishmania infection in mixed C57Bl/6xBalb/c mice

I am having a hard time finding literature reports on infection of C57Bl/6xBalb/c mice with Leishmania major. If I have an F1 generation of mice from C57Bl/6 and Balb/c, and infect them with L....

20 May 2014 7,016 2 View

Is there any method that uses private haplotypes as a measure of population genetic structuring?

The traditional methods to detect population structure like Fst sometimes are not able to detect differences among locations or populations even when they exist. In a case where there seems to be...

23 January 2014 5,556 3 View

Can monoclonal antibodies be used as anti-infective agents?

Seeking antibodies directed against bacterial or viral proteins that have proof of efficacy

28 July 2013 9,013 1 View

Which plasmid to use for expression of two proteins?

We are interested in expressing two proteins from the same plasmid. Which vector is the best? Is it possible to use the same promoter for both? We are not interested in bicistronic, but in a...

27 May 2013 4,189 7 View

How can I get my article published in an A level journal on HRM/Organizational Behaviour? Any tips/experiences?

Is there anyone who is experienced in publishing in A level journals? Any tips will be appreciated. Thank you.

01 January 1970 1,385 13 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

It is necessary to reflux the Schiff base and metal salts to obtain metal complex??

24 February 2021 9,166 3 View