The systematic study of the complete complement of proteins (PROTEOME) of organisms. | Contact experts in Proteomics to get answers
1,236 views 732 posts
Questions related to Proteomics
Between Orbitrap Fusion (Thermo) and 6550 iFunnel Q-TOF LC/MSSystem (Agilent) which one is better for proteomic analysis? And the best software that I can use for data analysis especially if I'm...
14 December 2016 6,403 3 View
I am working on 4 bacterial strains I need the proteins that are present in all 4 strains for this I need guidance.... I did now how to do this step
01 December 2016 6,401 2 View
I came to know that Tris having primary amine group may interact with iTRAQ labels, and TEAB is an advised buffer to be used post precipitation; but if I don't have TEAB at present can I use...
25 November 2016 3,555 3 View
Dear colleagues, Following SCX, we've performed manual desalting of the individually-separated iTRAQ peptide fractions before LC-MS/MS. We used the commercially-available C18 Pipette Tips for the...
24 November 2016 5,088 5 View
In a study, we need to determine blood based protein biomarkers in stroke using proteomics techniques like MALDI-TOF-MS and 2D gel electrophoresis. So, which component of the blood should we use...
16 November 2016 413 3 View
I'm trying to do a proteomics analysis in synaptosome, but I'm not sure 2-D gel is a good method. Some paper indicated that a cationic 16-BAC polyacrylamide gel system should be used for...
15 November 2016 1,840 1 View
What is your opinion on batch correction in quantitative proteomics data sets. When do you use it? What is the reasoning/measure. How do you apply it to your data? How do you set up your...
03 November 2016 1,161 5 View
Which is the right loading control for cell culture supernatants while running WB? We are validating proteomics candidates from cell culture supernatants. The culture media was supplemented with...
31 October 2016 1,553 3 View
I am performing an MRM assay to target specific Salmonella proteins.
19 October 2016 7,290 2 View
Considering the total contents of a cell or tissue lysate (of your liking), would the lysate contain more proteins or metabolites? I have an impression it contains more protein than metabolites....
12 October 2016 1,146 2 View
I'll be using 2D gel to isolate proteins, but after that I'm not too sure how to proceed (aside from mass-spec). A comparison with actual patient leukemic cells will also be performed. I'd...
07 October 2016 228 1 View
MALDI TOF(Protein)Data format available in 1. raw format, 2. in MZ, 3. mzXML how to do peptide mass fingerprinting using it. How to use MASCOT AND PROFOUND database for analysis using MAVEN OR...
30 September 2016 2,244 3 View
As am curious, Is it we have started developing other technology like DIA, Target and Semi-Target Proteomics study, Due to Missing value issue. Do we have any stable tool to solve the Missing...
29 September 2016 992 2 View
We want to measure breath isoprene on a small (20 ml) volume of collected breath but would like to collaborate/contract with a lab that has the analytical facility/expertise rather than buying a...
27 September 2016 3,144 1 View
As the title describe, I wanna ask if anybody has known any method to eliminate the NTPs/dNTPs from the protein without nucleotiges that I use specific enzyme and ultraphonic method to broke them...
25 September 2016 8,159 3 View
Hi everyone, I am currently working with mzdata.xml files for protein identification through Mascot. I now need to perform de novo sequencing but softwares (e.g. pepnovo) don't accept this format...
23 September 2016 2,254 3 View
Hi, according to a pdf by Thermo, advertising the features of the Orbitrap Elite, stepped collisional energy is also available for this instrument. I always thought that this way of fragmentation...
20 September 2016 7,853 1 View
In my proteomic results, there is change in glycolysis and TCA and i want to know that which pathway is involved in changing these major metabolic processes. For that i want to do some more...
19 September 2016 492 3 View
I am facing one problem with identification carried out using Protein Pilot, I get many hits whose description shows reverse protein. Does anyone have any explanation for this? Use of Mascot...
18 September 2016 204 6 View
Hi, I`m looking for a plasmid to subclone into a mammalian system. It should have V5 and a His tag on N-terminus. Any suggestions?
15 September 2016 2,719 5 View
I am interested in calcium waves orchestrating repair and to study the contribution of certain calcium waves, I want to inhibit calcium in a very short time period after injury. I have...
14 September 2016 2,247 2 View
I can't use any nuclease because I need to purify RNPs. The RNA fragments are about 100nt.
06 September 2016 4,605 3 View
I saw a protocol for recombinant protein expression that recommended working in larger volumes (1L cultures) without antibiotics after inoculating overnight in antibiotics. How practical can this be?
06 September 2016 8,190 3 View
I am studying differential protein expression in microalgae under stress using 1 D proteomics. However, when I match the proteins with NCBI database using MASCOT software, the proteins are not...
02 September 2016 7,247 7 View