The systematic study of the complete complement of proteins (PROTEOME) of organisms. | Contact experts in Proteomics to get answers
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Questions related to Proteomics
We are spending more than the cost of the service contract every year on buying 'zero air' cylinders for the electrospray sheath gas. We have the 5600 and 6600 with NanoSpray III sources. We...
01 November 2017 4,402 3 View
Has anyone performed proteomics to determine the origin of exosomes in plasma? Can you please explain to me how did you performed it? Thanks :)
31 October 2017 4,403 2 View
I need to compare between metal treated and untreated bacterial samples.We have LC-MS facility which determines only molecular weight.Thanks
25 October 2017 3,267 6 View
Hi I have money to buy one of these for both metabolomics and proteomics. How stable are these instruments especially the 5600 model as regards mass accuracy? Thanks Brendan
18 October 2017 1,251 3 View
Our lab bought a qtrap 6500+ coupled with ESI sorgent and UHPLC. I know that instrument is exelent for target proteomic, but i want to know if it can be used for proteomic discovery.
12 October 2017 6,637 4 View
Hello, I am currently working on a project related to study of the intact protein by Top Down Proteomics. Proteins are fractionated by Gelfree 8100 Fractionation Station and after fractional...
03 October 2017 6,486 7 View
Hi, I am currently trying to compare a proteome dataset using only a small selection of protein abundances. Specifically, I want to use a selection of 21 proteins, sum the intensities of these...
22 September 2017 1,399 3 View
Just as showed in attached picture, in a 120min LC-MS run, there is a high and wide peak group in about 15-30min and in the rest of the elution time, the peak intensity is low which means that...
18 September 2017 6,054 5 View
Is anyone aware of a good proteomic study aimed at identifying endogenously biotinylated proteins? I know that a handful of mitochondrial proteins are known to have biotin cofactors, but wondered...
11 September 2017 1,421 3 View
It seems most cases od PRM are based on QE series and Fusion or Fusion lumos. I am just wondering if the Elite suitable for PRM? I know the scaning speed of Eilte may be a limmitation but now I...
06 September 2017 6,189 3 View
Our group plans on using the same tissue sample for MS-based proteomics and lipidomics. Our current method for protein extraction is using RIPA buffer to homogenize followed by the FASP protocol....
04 September 2017 7,837 4 View
Hi, does anyone have an idea what might be causing this dark brown (pale, clear yellow if I re-suspend) precipitate to form? Here is some background on my sample preparation: 1. Lyse HEK293 cells...
30 August 2017 8,962 7 View
Is there a comprehensive (perhaps proteomics-based) approach that will screen out autoantibodies from blood and also (reliably) predict their target? I am interested in autoantibodies against CNS...
18 August 2017 883 1 View
Can we use ProteoMiner kit from Bio-Rad for comparative proteomics studies to know differentially expressed proteins? Which high abundant protein depletion kit is best suitable for comparative...
16 August 2017 5,930 4 View
The software should work like this: I choose a molecule; and then the software scans a whole library of many many molecules, and then it quantifies the overlap of each one of them with my target...
16 August 2017 2,377 3 View
I am over expressing BirA fused to my protein of interest in 293 cells, doing pull downs with streptavidin beads and sending for mass spec. I still don't have any results even though BirA-POI is...
03 August 2017 890 1 View
Does anybody have some good papers on peptide peak area between-sample repeatability after treatment with trypsin (i.e. bottom-up proteomics)? Needs to be for complex samples, and not just...
04 July 2017 4,568 2 View
Dearests, I am working on Proteomics and want to check the immune response (changes) brought by a challenge from a pathogen to the honeybee larvae based on hemolymph samples. Furthermore, I want...
11 May 2017 4,508 3 View
I performed virtual screening of the natural products from the ZINC library with the aim to identify compounds against the b5 active site of the proteasome. Carfilzomib and epoxomicin were...
25 March 2017 1,675 3 View
We are studying swine (pig) plasma samples for proteomics. However, as long as we understand, there is no reliable commercial kit to deplete albumin and IgG from swine plasma. I wonder if anyone...
14 February 2017 5,954 3 View
Hi, I wonder if any of you is working in commercial clinical proteomics laboratory using LC-MS/MS methods. Please let me know. Thank you
20 January 2017 7,425 4 View
Its general questions also about the quality of results between the two methods?what about the total protein ID?for example if I performed the two methods for 2 protein mixtures, in one of the...
18 January 2017 3,591 3 View
proteomics
17 January 2017 7,813 3 View
20 December 2016 8,002 3 View