The systematic study of the complete complement of proteins (PROTEOME) of organisms. | Contact experts in Proteomics to get answers
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Questions related to Proteomics
We have thought about glass beads and sonication: Is there a frequency one can use to sonicate and lyse open eukaryotic cells but leave the bacteria intact? Alternatively, is it reasonable to...
09 November 2015 6,381 5 View
Dear colleagues, Hi. I am new to LC-MS/MS proteomics and would like to seek you advice on a few LC configuration: Is peptide pre-concentration using the trap column a must (in the LC-MS/MS first...
02 November 2015 7,762 9 View
Hi guys,I'm working with bacterial cell pellet and would like to discuss what the possible causes of the occurrence of a few spots on 2D gel electrophoresis. I used at 400 ug protein strip pH...
16 October 2015 9,657 13 View
I have proteomics data and metabolomics data of the same sample set of a disease model. I have performed network/pathway analysis to each platform, but is there a good ways/tools/softwares to...
28 September 2015 4,893 1 View
Phenotypic markers have been assessed and we see profound difference.
21 September 2015 9,503 3 View
I performed silver staining using a standard protocol. But the problem is As soon as I add silver nitrate to gel, the solution turns white and the gel becomes white. What could be the reason for...
15 September 2015 8,133 4 View
I am reading some articles about use of ion mobility-mass spectrometry for determination of dynamics of proteins. I am not able to understand how to convert mass data to the structure of any state...
15 September 2015 3,492 3 View
I am trying to extract some cell-specific transcriptomic/proteomic data for cancerous tissues from Human Protein Atlas, however, it did not work out. I appreciate any cues in doing so, and if...
15 August 2015 5,356 4 View
Dear Researchers, DNAbinder, DNABIND and HMMER are useful for web servers for predicting DNA-binding proteins and they take many sequences at a time. BindN, DR_bind, DNA Bind Prot, metaDBSite, and...
16 July 2015 8,363 3 View
Dear colleagues, With access to only one Orbitrap instrument, we are conceiving 2 different LC systems for switching between proteomics (nano-UHPLC) and metabolomics (UHPLC) analyses once a...
27 June 2015 5,024 5 View
When I run SDS PAGE gel and perform zymogram with same gel.I observed that the bands which are more intense (more protein) after CBB staining give less intense activity bands in zymogram, whereas...
26 June 2015 2,388 6 View
I have a set of proteins of interest from a non-model organism (the beautiful marine bivalve Ruditapes philippinarum) and I would like to "predict" / map possible protein-protein...
22 June 2015 9,867 3 View
To distinguish protein splicing isoform seems to be quite difficult by using mass spectrometry, because of the nearly identical sequence of them. And after fragmentation in mass spectrometry,...
13 June 2015 2,915 7 View
Hi everyone, I am planning to do TMT labeling on digested peptides. I read the manual from Thermo. It seem that the manual doesn't mention anything about whether the digestion reaction should be...
11 June 2015 382 4 View
After doing in solution trypsin digestion of proteins and bringing up my sample to 5% ACN and 1% Formic acid to run it on the MS I end up with a white precipitate after couple of hours. If I do a...
01 June 2015 7,437 11 View
I have a proteomics dataset and would like to target some proteins from the dataset with some inhibitors. Is there any repository which provides a list of proteins and small inhibitory molecules?
26 May 2015 3,884 7 View
I would like to research the poplar resistance to stress by chloroplast proteomics method. First of all, I have to extract the leaf chloroplast. Dose anyone know the method of exacting leaf...
19 May 2015 661 1 View
Hi everyone, Currently I mixed equal amounts of cell from SILAC experiment and freeze in -80. I'm planning to follow general protocol with double digestion (LysC+trypsin) and IP with...
06 May 2015 3,430 3 View
I have extracted a metal-peptide conjugate in precipitate form by methanol extraction. However, to continue further experiments on it, the precipitates must be dissolved in a...
05 May 2015 9,148 4 View
I have been trying to identify proteins extracted from soil using tandem mass spectrometry. I am analysing the tryptic digest ms/ms data using different databases such as NCBI nr, swissprot, NCBI...
05 May 2015 8,987 10 View
Hi, If you have any answers and suggestions in the questions. Please let me know.
04 May 2015 767 3 View
to identify any protein, is it enough to have its average MW from ESI_MS spectra. or it has to be sequenced ??
28 April 2015 8,822 8 View
A threonine residue can be alkylated during an alkylation process using iodoacetamide if overalkylation occurs in the protein?
24 April 2015 2,923 3 View
Hi everybody! I have to do an EMSA to see if my mutations in my protein of interest abolished binding to DNA. Foe lack of time and of permissions of using radioactive labels my boss suggested to...
23 April 2015 4,472 3 View