The systematic study of the complete complement of proteins (PROTEOME) of organisms. | Contact experts in Proteomics to get answers
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Questions related to Proteomics
This question is concerned with the study about interferon response in human hepatoma cell line (Huh7). After doing proteomic analysis using ICAT and tandem mass spectrometry (MS/MS), this data is...
22 April 2015 9,043 2 View
Hi I am working in a lab which does extensive metabolite analysis using triplequad (Bruker EVOQ Elite) and QTOF (Bruker Compact) instruments. We are about to set up a method for measuring peptides...
20 April 2015 3,142 6 View
Can anyone give advice on current thinking with respect to pooling samples across individuals prior to performing RNAseq or Proteomics analysis. We are planning to study transcriptomic and...
16 April 2015 9,807 18 View
My protein of interest has a disulfide bonds, and it's a transmembrane protein. However, studies showed that this protein localise not only in the plasma membrane, but also found intracellular...
14 April 2015 8,706 3 View
I might have overlooked something, but I could not find any information on what the maximum concentration of sodium deoxycholate in my buffer can be for StrepTagII-Streptavidin purification. In my...
09 April 2015 3,682 3 View
A hypothetical question. I have two closely related plants. One is suspected to lack a certain protein present in the other. How difficult is it to discover this difference in the following...
01 April 2015 6,339 6 View
I have used the X!tandem software for proteomics data search and couldn't get the correct output for my current settings below. Residue modifications-> 144.1021@K Residue potential...
27 March 2015 8,966 6 View
I'm successful in separating the presynaptic and PSD fraction using a method that published in a Neuron article. I have some native gel running complication with the pre-synaptic membrane fraction...
24 March 2015 517 2 View
I´m able to induce somatic embryogenesis with leaves and seeds in the same species. If I realize the proteome analysis, could i compare the two tissues during the induce process? i.e., two...
23 March 2015 4,572 3 View
I am interested in doing a meta analysis study based on already published literature especially using protein based data to test one of my hypothesis and bring out the signalling networks acting...
23 March 2015 2,675 2 View
Hi, I am trying to analyse proteomic data in PLGS from waters. I have quantitative data, so need to process in DDA mode but in workflow parameters, there was no option for FDR. Can any one suggest...
19 March 2015 2,699 3 View
I have extracted surface layer protein from lactobacillus with 5M LiCl, dialysis against distilled water for overnight (approximately 20 hours) (5-10C), and freeze dry them. However, I found that...
19 March 2015 4,657 5 View
Coming from a background where internal standards is an absolute must for quantification, I wonder about spectral counting in proteomics. How can this work properly, considering ion suppression...
19 March 2015 5,164 6 View
Hi,I am trying to measure the content of methylglyoxal-dependent damage on arginine residues in my protein samples. I know an extensive protein hydrolysis must be carried out in order to...
18 March 2015 8,826 2 View
I'm working on the ubiquitination of a protein and to understand the type of ubiquitination, I used MG-132 and lactacystin proteasome inhibitors. Interestingly, I have a huge decrease of my...
17 March 2015 4,567 19 View
Thank you.
17 March 2015 8,448 3 View
I am having problems with gradient formation. I am using 6 mL of Percoll 80%, over it 6mL Percoll 35% and then in the top the buffer resuspension approx 35 mL (leaves extract in NIB) -> all in...
17 March 2015 8,823 2 View
Hi everyone, I would like to find out peoples' opinions on the need to do validation of protein expression in a proteomics analysis (by western blotting for example). I understand that this was...
17 March 2015 1,050 8 View
Hi to all, I have prepared a protein complex made from two monomers keep together by a metal, I'd like to analyse it by reverse phase HPLC using a gradient from 2% of ACN, to 100%. i have some...
13 March 2015 8,156 6 View
I would like to now the best validation methods for proteomic data obtained during osteoblasts differentiation cultured in vitro
11 March 2015 1,662 7 View
We all know that all proteins at a pH equal to their PI tend to precipitate. So: Is there any protein in our body that has a PI equal to physiologic pH (7.35)?
08 March 2015 2,499 5 View
There are many proteins that have structured domains as well as unstructured regions. The unstructured regions are important in functional regulation e.g via post-translational modifications. I...
03 March 2015 418 3 View
Hi, it should be possible to enhance your confidence in PTM site localization by combining information from digests with different proteases, e.g. by considering where the peptides overlap and...
02 March 2015 3,728 3 View
Hi to all, I have to perform a trypsin in-solution digestion of a protein sample. I would like to avoid the use of SDS/Urea/GnCl. Is possible to perform the trypsin digestion of my proteing...
27 February 2015 8,172 10 View