The systematic study of the complete complement of proteins (PROTEOME) of organisms. | Contact experts in Proteomics to get answers
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Questions related to Proteomics
I performed some comparitive proteomics between planktonic and biofilm membrane proteomes using tandem mass tags. The mass spectrometry generated HCID and CID data . I now need to identify all...
14 August 2018 6,091 3 View
I have a number of proteomics research datasets which I have analyzed in MaxQuant. To make the data publicly available, I typically upload the data to ProteomeXChange through the PRIDE repository...
07 August 2018 9,645 10 View
I'd be thankful if anyone suggest me the best open source software for analyzing free-label quantitative protein data-set.
28 July 2018 5,097 2 View
Hi friends, After doing LC-MS MS what threshold fold change do researchers usually consider for up and down regulation? I mean, what is the significant fold change? In some paper 1.6 fold change...
16 July 2018 1,079 9 View
Hello! We are proteomics group that would like to buy a new mass spectrometer that would be used for simpler samples. For example, to detect 10-20 peptides. We currently have QE with nanoLC that...
05 July 2018 2,650 6 View
Ziptip vs C18 tip (Pierce) vs Stage tip (made in house) vs C18 spin tip/column (Pierce)
19 June 2018 8,529 5 View
I was under the impression that one of the benefits of LysC is that it is active under high urea concentrations (8M) retaining protein denaturation in hard to digest proteins (such as ECM). PNGase...
12 June 2018 7,916 2 View
As the question, I don't know that what difference between spatial proteomics and affinity enrichment (AE) or purification (AP)-MS in interactomics. Anyone can help?
08 June 2018 7,978 3 View
Hellow everyone. I am supposed to use EMBL pride proteomics documents. But I don't know how to use EMBL pride inspector program. even there is no file to introduce inspector program. Up to now, I...
30 May 2018 4,849 2 View
Dear Colleagues, I am required to submit my proteomics MS data into PRIDE. But when I checked my data, I found I only have the peak list (txt) and the search result (html) but no so called RAW...
24 May 2018 795 5 View
First of all I have to say that I am newbie with Max Quant haha. I have been using other Proteomic software like Proteome Discoverer and Scaffold. I have got 6 samples ( 3 control biological...
11 April 2018 8,284 2 View
Could anyone please suggest the best material source for reading and understanding the Proteomics science for a naive person to Proteomics? Thanks,
10 April 2018 8,224 2 View
I am doing lots of proteomics and hence I often times perform speed vac. I do not want to dry my samples completely, but I want a certain fixed volume left (lets say 100ul). Has anyone found a way...
10 April 2018 7,650 4 View
Hi, I need to place an order but i don't want to order the wrong product, since is the first time I approach to this kind of experiment. After some research I found two products that could be...
05 April 2018 7,599 8 View
Dear colleagues, I detected some proteins in a tissue but the gene mostly expressed in another tissue. I guess the protein might be transported from the original tissue. How can I prove it or how...
12 February 2018 9,374 4 View
I am working on Cotton drought tolerance. i am now doing the proteomics and metabolomics related things. What kind of new things I can do further that means any kind of transcriptomics or other...
05 February 2018 9,729 3 View
phosphoproteomic data analysis
29 January 2018 804 4 View
input: amino acid sequence output: potential protein binding partners to sequence Thanks for taking the time to address my question. Bre
25 January 2018 2,260 4 View
Optimally, it would contain data from a sample for several instruments tracking performance over time, possibly with annotation.
15 January 2018 7,687 3 View
Currently I am working with rat blood serum. Instead of metabolomic or proteomic, can I get opinions on any other analysis which can be conducted using the serum (at least strong enough to use...
22 November 2017 4,132 3 View
The aim: Use of ORFs predicted by Transdecoder from assembled tarnscriptome, as protein sequence database for proteomic study of non-model organism (proteins identification by...
22 November 2017 9,148 2 View
Hi everyone. I'm working on the project for protein identification using LC-MS1 spectra without using any tandem mass spectra. I want to calculate economical benefits from using this approach....
21 November 2017 1,868 3 View
I want to perform O2PLS-DA analysis of multiomics data (from different metabolomics lipidomics and proteomics experiments) by using SIMCA 130.2? I have data in matrix format (samples in row with...
17 November 2017 8,904 3 View
I have proteomics data for the bacterial proteome expressed under two different conditions. Now i have list of common, unique and differentially expressed proteins with their UniProt ID in...
10 November 2017 4,641 6 View