The systematic study of the complete complement of proteins (PROTEOME) of organisms. | Contact experts in Proteomics to get answers
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Questions related to Proteomics
In the Specify Processing tab, in the ID Focus I can select biological modifications and aa substitutions, but I don´t know how to know which ones the program is using
04 April 2016 3,686 1 View
2de proteomics researchers
03 April 2016 8,620 2 View
I think I read some phosphoproteomics papers in the late 2000s where people would quantify differences in enriched phosphopeptides using differential isotopic labeling of acids with esterification...
24 March 2016 2,606 3 View
i am search for urinary biomarker in acute rejection in kidney transplant recipients.
09 March 2016 8,866 2 View
I would like to collect samples (leaves, stem and roots) from the field for my proteomics study,
08 March 2016 5,223 3 View
During a process of deep purple staining of 2-D protein gel for automated Ettan DIGE spot picker, my gel was unstuck with the backing (Plate). I can’t scan the gel as it is not fix with backing....
03 March 2016 2,858 1 View
I'm writing a chapter on protein inference/grouping, and I want to describe how the main packages perform this process. Anyone provider a pointer to docs or a paper? Thanks Andy
29 February 2016 6,518 3 View
Hi everyone, I have a list of proteins upregulated in cells under stress condition, when I compared the expression against control cells with Reactome I find some specific upregulated...
29 February 2016 2,841 2 View
Dear colleagues, For proteomics experiments, we've purchased LC-MS grade solvents. These high purity solvents initially have already been 0.1-micron filtered (by the vendor). To play safe,...
29 February 2016 1,600 10 View
Hi to everybody. We are working in the field of marine proteomics and decided to use thermolysin instead of trypsin etc for digestion (bottom-up, using LC-MSMS; 4600 tripletof). Unfortunately the...
25 February 2016 7,461 2 View
I just want to know what clustering algorithm will go good for proteomic data in mapreduce?
24 February 2016 6,964 3 View
24 February 2016 5,688 1 View
I'm doing the homology modelling using YASARA for my protein (it's a DNA polymerase from geobacillus). The model show the front tail after I adding the amino acid myself which was belong to my...
24 February 2016 3,696 6 View
Can anyone think of a potential reason why dasherGFP can be expressed just fine in a cell-free system, while cometGFP doesn't seem to work? I'm not sure if it could be a translation problem or a...
23 February 2016 2,323 4 View
any one who have an experience on Protenomics
12 February 2016 7,907 2 View
NA
10 February 2016 2,044 3 View
Read 9 answers by scientists to the question asked by Mani Balan on Feb 5, 2016
05 February 2016 1,752 6 View
In Proteomics, while doing analysis on protein pilot after running my sample on mass spec; I got plenty of protein IDs which are having zero peptide identification to the library. I don't...
15 January 2016 5,067 3 View
Hello! After deconvolution of intact mAb mass spectrum I saw a mass difference between peaks about 113 Da. The mass of the smallest sugar residue, fucose, is about 164 Da. Can anyone explain what...
24 December 2015 5,500 5 View
I would like to know if it is important to add protease inhibitor to plasma when using EDTA tube for a proteomic study? If so, what is the suggested brand? Thanks
08 December 2015 5,877 10 View
Currently, i'm working on secretome study and i use 2D to evaluate the protein. Unfortunately, i've got no spots after staining the gel with commasive blue. My concentration was 400ug and i used...
03 December 2015 7,491 10 View
I am working with Waters Xevo GS-2 Q-TOF mass spectrometer in MSe mode to analyze and identify tryptic peptides. are progenesis QI for proteomics or waters PLGS the only software that can analyze...
02 December 2015 3,937 14 View
We have a proteomic dataset where many proteins have been identified in samples derived from two classes of samples with three biological replicates each. Each of these samples was analysed...
26 November 2015 1,046 4 View
I would like to use snap frozen tissue samples for proteome analysis in Mass Spectrometry Imaging but they have been emedded in Tissue-Tek during procurement. Due to certain circumstances and a...
23 November 2015 258 4 View