I am interested in calcium waves orchestrating repair and to study the contribution of certain calcium waves, I want to inhibit calcium in a very short time period after injury.
I have used BAPTA-AM which inhibits calcium very effectively but obviously give me long-term side effects (I am working in a developing organism in vivo).
I recently discovered Diazo2-AM which shows a strong increase for calcium binding after illumination of light with a certain wavelength - that would be ideal for me, sadly this product has been discontinued.
Is there any alternative I could try or are there other ways of doing so that I haven't thought of?
Thank you for your help.
Mg2+ is a weak inhibitor of Ca2+ influx. If in your case Ca2+ waves are triggered by Ca2+ influx from outside, increase by 2x the [Mg2+]o you may find that the Ca2+ spike will stop.
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