The systematic study of the complete complement of proteins (PROTEOME) of organisms. | Contact experts in Proteomics to get answers
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Questions related to Proteomics
Dear colleagues, is there a database which contents the correspondence between gene id and protein id for a species? (I have two data sets for the same organism, including either gene id or...
28 August 2016 839 6 View
Dear all I tried a protein purification with L-Arginine 0,4M but my protein doesnt bind in HisTrap, then i reduced it to 0,2M and in the rest of the buffers I used a 0,4M concentration. With this...
25 August 2016 925 3 View
Dear colleagues, Our lab is testing a new freeze-dryer's performance, but we are not sure if we've been using it correctly. It's a benchtop type, with a transparent drum manifold, operable at...
01 August 2016 7,549 12 View
Hi, I'm looking for a free programme that will allow me to superimpose a short protein sequence/a motif on its structure or a structural model to verify if it's water accessible or not. A...
29 July 2016 2,029 4 View
I´m trying to express one product in Pichia pastoris but this organism is cleaving it. I added PMSF to the expression medium but it was not effective. Did anyone have this problem?
28 July 2016 5,626 4 View
Sustrate=BAPNA I get a white solution when i mixed BAPNA(with DMSO) and buffer Tris-HCL/CaCl2 First 3 minutes the activity goes down, after 3 min stars to go up but slowly. Is this normal?
26 July 2016 6,815 7 View
I am looking for an anti-NHE1 primary antibody. I ordered a polyclonal antibody from ThermoFIsher Scientific but when I tested it (WB); it was not specific enough for the proteomics work I need it...
25 July 2016 6,208 2 View
i am using Agarose Concavalin A for elution in spin column.
22 July 2016 4,749 2 View
We are having problems with an ESI LC MS/MS proteomics experiment that are difficult to pin down. Samples were prepared in batches over the course of a week, the first batches worked very well but...
13 July 2016 9,523 10 View
I have tagged my protein of interest with mCherry at N terminus and have episomally expressed in Leishmania promastigotes. I observed even in live cell fluorescence stars quenching within few...
13 July 2016 1,096 3 View
After the identification of numerous proteins to be deregulated in a diseased tissue using iTRAQ what should be the criteria to select a few proteins for their validation using Western...
10 July 2016 1,997 3 View
Hi, Is it possible to measure the protein concentration after digestion with Proteinase-K e.g. using BCA method? When compared to homogenized samples without Proteinase-K, Proteinase K digest...
08 July 2016 8,590 3 View
I need that my protein after expression contains C-terminal His-tag and cleavage site before this His-tag (Tev protease / Thrombin ) without any addition signal sequenses and tags on the...
25 June 2016 4,305 3 View
I have genomic, metabolic and proteomic data from bacteria and I'm attempting to integrate them with plant physiologic and transcriptomic data. But actually I'm facing with many problems, like the...
21 June 2016 2,814 2 View
I want to know simple method of assay and related substance analysis for Amino acid. If anyone have experience of analysis of these then please let me know. I need this method.
11 June 2016 4,112 5 View
I performed a phosphopeptide enrichment using TiO2 beads, which worked well, but because of the very limited sample I had, I could not keep a fraction before hand to use for proteomics to compare...
09 June 2016 2,793 2 View
I'm going to get the bacterial outer membrane proteins, but I'm just starting about the protein handling. The protocol is following. 1. Harvest cell 6,000xg at 4'C for 10 min 2. Washing the pellet...
31 May 2016 7,005 5 View
in FASP (Filter assisted sample preperation) prosedure, the enzyme to protein ratio needed for digestion of a protein sample is 1:100, if it is used over -use trypsin, what kind of problem can be...
23 May 2016 3,716 9 View
Labeling is done using cyanoborohydride, formaldehyde (both deuterated and normal) in a phosphate buffer for 1 hour at RT in solution. It's, ofcourse, mass spectrometry related. ;)
20 May 2016 8,889 12 View
Dear Experts, As I am doing proteomic profiling for particular fraction of cell. By following the protocol I prepared my sample for LC-MS/MS analysis but the concentration of protein in that...
19 May 2016 7,171 4 View
I'm planning on analyzing phosphopeptides from tissue samples. I was told to extract protein from tissue using either 8M urea buffer with 0.1% NP-40, 1 mM sodium vanadate and protease inhibitor +...
22 April 2016 7,576 9 View
Macrophage can engulf 1 to 30 red blood cells at once. Large erythrophagosomes are observable in the cells for a few hours (up to 7-10 h). What subcellular fractionation approach would be most...
14 April 2016 7,191 2 View
one protein can have more than one domain..... sometimes one function is perfomed by combination of domains.....
06 April 2016 3,920 1 View
some servers gives results but do not show energy or e value of protein ligand docking. can anybody help.
05 April 2016 5,233 1 View