Hi, I`m looking for a plasmid to subclone into a mammalian system. It should have V5 and a His tag on N-terminus. Any suggestions?
Can try:
The pEF6/V5-His TOPO® TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO® Cloning. The pEF6/V5-His-TOPO® vector includes:
Enhancer/promoter elements from the human elongation factor 1α subunit (hEF-1α) for high-level expression in mammalian cells
• The Blasticidin resistance gene for rapid selection of stable mammalian cell lines
• A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody
• A C-terminal polyhistidine (6xHis) sequence for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term)
• Antibody
Thanks Kai, I have TOPO TA, but it`s C terminal His Taq and I need N-term.
http://www.oxfordgenetics.com/Products/Plasmids/Details/Mammalian/pSF-CMV-NH2-V5-EKT-Nco1-(OG91)-N-Terminal-V5-Tag-Plasmid/OG91
Product Description
N terminal V5 peptide tag expression plasmid for adding the V5 epitope to the end of a protein of interest. The tag is adjacent to the NcoI site.
BTW, can you use N-Terminal-GFP tag instead?
i.e. pmaxgfp-green-N-vector (I have this one)
Hi Lenka,
I'm looking for a mammalian expression plasmid with V5-His on N terminal? Did you manage to find one? If so, Do kindly let me know.
Many thanks
Sudharshan
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