Which seems to be a better option: using Plasma or Serum, for determining blood based protein biomarkers using MALDI-TOF & 2D?

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Ram Sagar

Serum is a better option for MALDI-TOF because Serum can give a good proteome profiling result of the LMW fraction of serum.

Steingrimur Stefansson

Hi Shubham, I agree with Ram about using serum instead of plasma.

But, are you on a fishing trip or are you looking for specific markers?

Shubham Misra

Hi Steingrimur,

Actually I am on a fishing trip here.Looking to assay an entire protein profile of patients to find out biomarkers in stroke. The study will consist of a discovery phase initially. And once the required biomarkers are found, they would be validated in different cohort of patients.