Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
1,576 views 4,169 posts
Questions related to Plasmids
what is tofu
14 March 2023 1,914 2 View
Hi there, I have been putting together an enzyme assay that measures the conversion of one sugar substrate into another sugar substrate of a reductase, with the goal of coupling enzyme 1's...
14 March 2023 5,086 2 View
Planning to run my assays this week, but not sure if I will be able to tomorrow. I want to prep as much as possible ahead of time, and was wondering if I could coat my plates with 10ug/mL antigen...
12 March 2023 9,131 6 View
I used to normalize my target protein using housekeeping genes. But now I have come to know about total protein normalization. So I would like to do the same. I want to know how to measure total...
12 March 2023 9,480 2 View
Hello, I am young researcher working in computational drug discovery and willing to perform experimental work, for which I am planning to start with ITC. Currently, I am reading relevant...
11 March 2023 3,386 3 View
Hi Just wandering if anyone else is observing a rearrangement of a plasmid, with partial loss of the plasmid region that doesn't contain Ori, in plasmids that contain two or more of the...
10 March 2023 8,239 5 View
The plasmid i am isolating is a low copy number plasmid. I am supplementing the broth culture with appropriate antibiotics. Even then the plasmid quantity i am getting is not satisfactory. The...
10 March 2023 1,599 3 View
i need urgently. Thanks!! M
10 March 2023 6,287 3 View
The Kozak sequence is 5'-gccRccAUGG-3', but the only amino acids that can be coded for by codons that start with G are valine, alanine, aspartic acid, glutamic acid, and glycine, so how is...
09 March 2023 8,244 1 View
I am using epithelial, fibroblast and endothelial cells for my work following the standard cell culture procedures. However, I need to transport them for some other experimental work for at least...
09 March 2023 7,294 1 View
Hi, can anyone explain what is the difference between Midi pre, mini and maxi prep.
08 March 2023 4,727 2 View
How is microbial life increasing the formation of soil and role is played by rhizobium bacteria in increasing the fertility of soil?
08 March 2023 7,443 8 View
Hi all, I have been trying to synthesize luciferase self-amplifying RNA (saRNA) by in vitro transcription (IVT), and this vector has approximately 11kb. The main problem is that I am getting a...
07 March 2023 576 7 View
Is anybody familiar with polulation genetics? I have been reading a book of Population genetics Written by C.C.Li; there is a table shows the genotype and mating frequency change in a inbred...
06 March 2023 7,574 0 View
Why do you use a coating buffer containing carbonate / b-carbonate for ELISA? Do you use a combination of carbonate and bi-carbonate? Or do you just use one of them? If yes, when do you use...
06 March 2023 3,239 5 View
Hi! I am an undergraduate researcher at UC Davis working on culturing mammalian cells (MDCK and MEF specifically) in a PDMS (Sylgard 184) closed-channel microfluidic device (12 x 16 array of...
06 March 2023 3,128 0 View
Hi everyone! I'm trying to amplify a gene 900 bp long. I have the "right" band, but also a band with a higher length, under ~2000 bp. Once I purified the 900 bp band from gel, I ran the...
06 March 2023 8,779 4 View
..
03 March 2023 9,317 2 View
I am trying to establish a plaque assay for the H1N1 strain but can not make it after multiple tries. MDCK cells were used. Media I used for H1N1 growth and plaque assay stated below (for...
27 February 2023 8,715 2 View
My gastric cancer cells have already been transfected once with shRNA and gene knockdown has been verified. After a period of time in culture, the behavior of the cells changes. Can I re-knockdown...
27 February 2023 9,096 1 View
My 3 flasks of 293T cells have something in them that I've never seen before. It doesn't look like bacterial contamination or like fungus. I took a picture for you to see. There are tiny cells...
27 February 2023 2,555 1 View
Hello, What do you think of the subject on which I wrote a book in French and entitled: Everything that happens in the "head" of cells and microorganisms. Do cells and microorganisms have a “brain”?
26 February 2023 3,757 6 View
A colony PCR was run using bacterial colony of Salmonella Typhimurium but didn't get any band using 1% agarose. Anyone please help me out.
25 February 2023 8,711 5 View
Hi All, I am currently doing recombineering to insert two mutations on a plasmid, but after successfully recombining the sequence, the cells from my plates don't grow on LB broth. This is my...
25 February 2023 8,922 4 View