Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
Recently we obtained TCSPC system, but, spec of our main laser has pulse width 30-ps, and 50 Hz repetition rate. So, it seems the laser diode that give us the input time seems not detecting the...
22 November 2023 9,847 1 View
Hi, I'm curious about the exact organelle where ferroptosis takes place. Initially, I thought it was limited to the mitochondria and ER, but after reading a few papers, it seems it might occur in...
22 November 2023 2,173 2 View
Any project topic on Radiation Protection, Radiation Dosimetry in Radiotherapy or Brachytherapy for PhD studies?
22 November 2023 571 2 View
Hi, At some point of the media, I observe some things, I don’t recognize this shape. Does anyone have an idea? Thank you for your help!
20 November 2023 8,162 3 View
I have isolated plasmid and then linearised it with re. Then I put in-vitro transcription of the linearised plasmid but not band of mRNA could be observed only the linearised plasmid was seen....
20 November 2023 3,562 7 View
To study leaf, stem and root anatomy To study the change in the profile of endogenous phytohormones To study the change of activity speed of antioxidant enzymes
19 November 2023 6,338 6 View
There are several online servers today that predict B-Cell linear epitopes. This prediction is based on the human immune system. But is there any online server or offline software to predict the...
19 November 2023 8,061 1 View
I use zinc finger protein 179; I choose NdeI (forward primer) and HindIII as a reverse primer. but when I ran PCR and 1% agarose gel, I did not get the desire band. My protein was 1896 bp, but I...
17 November 2023 9,257 4 View
Hi, i want to produce a high titer of lentivirus using psPAX2, pVSVg, and third plasmid (my GOI cloned) but I am confused about what molar ratio will be best to get higher titer of lentivirus. i...
16 November 2023 9,309 2 View
I am little bit confused to understand whether all the niches where cancer cells remain dormant are antimetastatic niches? How will we consider the accumulation in the bones?
16 November 2023 4,217 4 View
transfection, lipofectamine 2000
15 November 2023 2,361 4 View
My thesis has been posted as a preprint on Research Square, but I am not interested to post before acceptance. How I can remove the article ?
14 November 2023 8,895 1 View
I used a established PCR kit to amplify the target DNA. The instructions from kits was given to use (1.5-15ng/ul) concentration of DNA. After DNA extraction procedure i got 23ng/ul;then i adjust...
14 November 2023 4,674 4 View
Hi everyone, I've ran a western on mouse ileal lysates and probed for COX-2. 50ug was loaded per sample and the dilution factor was 1:500 for the primary (monoclonal mouse ab) and 1:10000 for the...
14 November 2023 6,829 0 View
I conducted a co-immunoprecipitation using protein X antibody to determine if protein X is attached to protein Y. However, the results of my experiments were unusual. To ensure that the procedure...
13 November 2023 9,780 1 View
I tried a PCR protocol using 16s-rDNA universal primers and 55 degree for annealing and I got sharp specific bands in the exact place showing by ladder. After that I used the exact protocol again...
13 November 2023 3,156 6 View
Hi all, I've been using a blend of chitosan and PEO (dissolved in aqueous acetic acid) to produce electrospun nanofibers. Whilst I am able to produce bead-free nanofibers, I often see the...
13 November 2023 3,603 4 View
Hello. Can someone help with MIN6 cell culture. I'm culturing it with DMEM high glucose, 15% FBS, 50uM b-mercaptoethanol, 20mM HEPES an 1% antibiotics, however I fell like cells are growing to...
13 November 2023 2,978 1 View
I have been trying to design primers and one of the primer criterion given is that any possible hairpins have a negative delta G value. I am confused about this as I would assume that a more...
12 November 2023 6,187 1 View
I am optimizing a ligation step of a plasmid using a vector: 14801bp, 149ng/µl and an insert: 403bp, 280ng/µl. I need to know the molar concentrations of these DNAs so as to understand the molar...
12 November 2023 1,903 4 View
What bacteria are present in rhizospheric soil and what are rhizosphere and rhizoplane microflora and its significance in soil borne disease?
11 November 2023 3,360 2 View
Not been able to find any article on this. Help me to find out.
10 November 2023 1,519 1 View
Hello, I am doing a titration of ligand into protein and am getting good heat releases compared to my ligand into buffer. I am getting more heat release in later injections. Could cooperativity...
08 November 2023 740 4 View
I am doing a CRISPR targeting to knock out one intron of my gene of interest in a cancer cell line and replace that intron with GFP. I am wondering if I should pick single cell clone with possible...
08 November 2023 2,951 0 View