Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
I have cloned a 1 kb gene into a 5.3 kb vector (in between BamH1 & Xho I) and screening of the recombinant plasmid has been done by colony PCR using gene (insert) specific primer where I am...
22 June 2023 3,009 3 View
Stool examination while fresh. 58-year-old male subject with diarrhoea.
19 June 2023 2,908 0 View
Hello researchers, I carried out an extraction with methnol of roots and at the end of the extraction a precipitate has formed. Can anyone explain to me if this is normal? Should I analyse this...
16 June 2023 952 2 View
I am planning to HEK293 cells for drug treatment. I would want to know till what passage I could use these cells? Thank you
16 June 2023 882 2 View
Hello everyone, I'm currently tryning to overexpressed and purified a protein from saccharomyces cerevisiea into BL21 (DE3). I'm currently using the pRSET plasmid and normal BL21 (DE3) However,...
16 June 2023 7,459 2 View
Hi everyone, I am now trying to delete some genes of the Agrobacterium Tumefaciens K84 strain. I decided to use the in-frame deletion method using the pk18mobsacB plasmid which harbours sac...
15 June 2023 8,943 1 View
microbial culture
14 June 2023 9,315 8 View
I have been working on site-directed mutagenesis for a few months now, without success. I used a High-Fidelity DNA polymerase to amplify my vector with an insert and saw the target band after the...
14 June 2023 8,456 1 View
I grow my positive clones in liquid LB (100 ul) for 3-4 hours until the solution is turbid enough. I want to know how much of the template is sufficient to put in a colony PCR reaction since the...
14 June 2023 5,997 3 View
We are trying to express a recombinant protein that is made of two different subunits. We have a plasmid with the alpha subunit and another with the beta subunit, but they are derived from the...
13 June 2023 3,833 5 View
I am currently working on a thermostable polymerase which I am overexpressing in the BL21(DE3) strain. The polymerase has a histag at the N-terminus. However, during purification, I am not...
13 June 2023 2,435 4 View
I want to digest pET28a plasmid (5369 bp) with 2 restriction enzymes (RE)- EcoRI and XhoI for cloning. I checked the REs have single cutting site of each in the plasmid. However when I digest it...
11 June 2023 5,645 4 View
Hi the attach file are plasmid extraction done with alkaline lysis (G- bacteria ) without phenol - chloroform i need help and suggestion.from right to left (loaded 20ul DNA sample + 5ul Orange...
09 June 2023 7,201 4 View
I did Gel mobility shift assay to check whether my protein can bind with dsDNA. I ran ssDNA(44nt in length) as well as the dsDNA(44bp in length) on a 8% non denaturing gel and I found that the...
07 June 2023 8,646 0 View
Trying to calculate how much blood I would need to draw in order to get a sufficient number of neutrophils (from which extracellular vesicles will be obtained).
06 June 2023 5,728 2 View
I have to clone a cDNA to insert in pcdna3.1. The forward primer generated is having tm 83 and reverse primer has tm 67. I am not getting amplification from normal pcr, gradient pcr, hotstart pcr...
06 June 2023 1,090 6 View
Hello everyone, I need to construct plasmid with pCAMBIA1303 backbone and Tbch1 Promotor and Terminator (Pcbh1 - target gene - Tbch1). I tried to find cbh1 promotor and terminator but found only...
29 May 2023 7,564 0 View
Hello, for my master's thesis, I have been working with phage display. One of my experiments involved electroporating a pUC19 vector containing the M13 full length gene 3, and a helper phage...
29 May 2023 1,580 4 View
I am using U20S(osteosarcoma) cell line, when cell plate is about 50% confluent, some black dots are visible in media which has Brownian motion but when plate is 100% confluent dots vanished. It...
28 May 2023 6,105 3 View
Good morning everyone! I am a Biotechnology student and I am doing a project to verify the biocontrol capacity of actinobacteria, for this I must carry out the molecular identification of the...
28 May 2023 5,533 3 View
I am interested in just having active roots under the soil for a few months. Is this possible? Any ideas?
27 May 2023 6,319 2 View
I want to insert my gene to pCMV6-Entry(Origene company) plasmid by using SfaA I and Mlu I,
27 May 2023 3,999 0 View
If a bacterium grows vigorously forming bit slimy situation in N free agar plates , is it nitrogen fixing?
26 May 2023 5,580 0 View
We are developing a peptide ELISA for IgM and IgG detection. We infected mice with parasite 1; for cross-reactivity analyses, we infected mice with two parasites (groups 2 and 3) and included a...
25 May 2023 9,714 2 View