Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
2,997 views 5,889 posts
Questions related to Plasmids
Environmental Stress Response in Soybean Plants Soybean (Glycine max) is a vital crop worldwide, providing a significant source of protein and oil. However, soybean plants are often exposed to...
25 February 2025 6,885 2 View
Guselkumab in patients with moderately to severely active ul... https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(24)01927-5/abstract
24 February 2025 6,809 6 View
I'm assaying saliva samples using a competitive immunoassay with HRP-labeled steroid hormone on microplates and TMB as the chromogenic substrate with H2O2. However, I'm getting oxidation of TMB...
20 February 2025 322 6 View
I work with site-directed mutagenesis, modifying amino acids in ion channel sequences using the splice mutagenesis method. The mutated fragments are then ligated into the full-length channel....
19 February 2025 2,855 2 View
Hi, I'm a master's student who studying inflammtion. Because of my main research, I need a vagotomy as my professor advised me to do vagotomy for some mouse groups, but I have no senior students...
17 February 2025 1,949 0 View
Later this week I will be extracting the hippocampus from murine brains for subsequent RNA extraction and qPCR analysis. During my initial attempt, I encountered significant difficulty handling...
29 January 2025 8,453 1 View
Hi! I want to generate basolateral-out spheroids of Caco-2 cells. I have Matrigel at my disposal. I would appreciate any suggestions or protocols. Thanks!
25 January 2025 4,806 1 View
I have found 3 activity assay kits for microbial transglutaminase as mentioned below: ZediXclusive Microbial Transglutaminase Assay Kit chromogenic, Hydroxamate, Art. No. Z009, kit uses Z-Gln-Gly...
24 January 2025 4,575 2 View
In my lab, most scientist had max rcf(about 15000g?) for centrifuging cells when they performed RNA precipitation with Trizol. And cells are about 1*10^5 ~ 1*10^6. How much g force is the least...
24 January 2025 8,114 2 View
Hello everyone, I'm currently working on setting up a simple total internal reflection fluorescence (TIRF) microscope using a few lenses (f1 = -50 mm, f2 = 300 mm, f3 = 400 mm) and a 532 nm laser....
21 January 2025 2,104 0 View
My I find articles on art therapy for displaced women from Ukraine
18 January 2025 6,171 0 View
Yesterday I uploaded a CERN technical report but added the wrong Sandro Rossi, please help
18 January 2025 415 2 View
Hello! I've just started to work with iPSC cell lines, and I was wondering if there are any checks I could do to ensure that the matrigel coating of my 6 well plates has gone according to plan and...
15 January 2025 6,966 2 View
I am working on a diabetes project, and my mice come from routine lab maintenance. As a result, the timeline between each cage does not align, meaning I may need to perform STZ injections on very...
15 January 2025 980 2 View
My silver nanoparticles have surface hydroxyl group. Can EDC be used to bioconjugate enzymes to the nanoparticles?
13 January 2025 8,837 3 View
can centrifuging make salivary supernatant cell/microbe free (sterile)? pls can somebody give a link to such a study that carried out microbial analysis of salivary supernatant.
11 January 2025 7,621 2 View
Hi! I try to make EMSA to study RNA/protein complex. The RNA length is 427 bp and it is GC rich. I made RNA from in vitro transciption and DNAse treatment (Promega kit) and purifyed it with...
10 January 2025 4,219 1 View
Know about the journal
10 January 2025 8,611 2 View
I'm starting to run some chemiluminescent protein dot blots for a simple verification of tau pathology within our mouse model. We have validated that our AT8 antibody works using IF and DAB...
07 January 2025 3,424 1 View
I hope this message finds you well. I have read your work, "Inscription and Optimization of Fibre-Optic Long Period Gratings Using Electric Arc Discharge," with great interest. I noticed that you...
25 December 2024 520 0 View
International Journal of Molecular Sciences (IF 4.9) run the entitled Special Issue, belonging to the section "Physical Chemistry and Chemical Physics" to create collection of papers on relevant...
18 December 2024 3,625 0 View
I am using Q5 high fidelity Master mix to amplify cDNA products which are of sizes 1 kb-2 kb long
13 December 2024 416 6 View
Hi all, I have been trying to show changes in protein expression of my target after gapmer treatment. I used multiple gapmer ASOs and sirnas for my target. while all can reduce mRNA expression by...
13 December 2024 2,802 0 View
Dear all, I hope this doesn't come across as a naïve question, but in the laboratory, we use DMSO to dissolve flavonoids and other compounds which don't dissolve well in water. I am aware for a...
11 December 2024 6,487 2 View