Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
Hi all, I've never worked with E.coli strains that already carry a plasmid and tried to make competent cells from them.. is it simply the usual protocol for chemically competent cells, but add...
09 January 2024 2,514 3 View
I'd prefer not buying any separate commercial media because of the cost. Please suggest what growth factors or vitamins can I add to DMEM KO other than glutamax, penstrep and bFGF for the MSCs to...
09 January 2024 6,425 3 View
Hi, I have been trying to isolate RNA from neutrophils isolated from whole blood samples. I cannot seem to consistently get a good RIN using the Qiagen RNAeasy mini kit. Does anyone have any...
09 January 2024 4,978 5 View
Greetings Everyone, I need a non conductive adhesive which can be used to attach two materials having Dk of 2.2 and 4.4. Kindly let me know if any one is aware of adhesive material having Dk...
08 January 2024 2,993 3 View
I've done several experiments using western blotting to detect extracellular vesicle markers TSG101 and CD9. However, I consistently observe bands that are larger than the expected size. Notably,...
08 January 2024 7,259 2 View
I am planning to get some blood from patients to perform RNA seq (circular RNA which requires deep sequencing). I am wondering if anyone can share the protocol with details. I am also wondering...
08 January 2024 8,314 3 View
Greetings I am working with a plasmid encoding the restriction enzyme EcoRV. Do you know of E. coli strain resistance to EcoRV restriction?
07 January 2024 8,184 4 View
For RNA isolation, I use the phenol-chloroform extraction method. To avoid DNA contamination I've used the DNaseI enzyme. But I'm not sure how long after I can do the DNase treatment. Should I do...
07 January 2024 5,443 3 View
Impact factor
07 January 2024 5,566 2 View
I have data of G', G'', and Angular Frequency and I want to know how can I Calculate Phase Angle based on them?
06 January 2024 5,125 1 View
Impact factor of IJERR
06 January 2024 1,381 2 View
I`m trying to prepare a solution of water-cmc with the highest content of CMC possible for further processing, but I wanted to know how I can lower the viscosity so it will be easier to handle but...
05 January 2024 2,045 6 View
Hello dear researchers, i am generating a stable cell line of HEK293T cells using lentivirus, i want to ask about the puromycin kill curve,if i seed my cells in a 12-well plate on day 1, Q1) when...
05 January 2024 3,080 4 View
heamatology study
04 January 2024 6,586 5 View
I have a problem and am asking for advice.I am doing WB. For electrophoresis I use an 8% separating gel and a 4% thickening gel. The electrophoresis has 2 phases : 30 min-90V , 60 min 110V....
04 January 2024 8,581 1 View
I performed a transfection to obtain a recombinant virus that expresses my protein of interest, which is marked with a yellow fluorescent protein (YFP). The PCR results showed both the band of the...
03 January 2024 8,383 5 View
Explore the intersection of machine learning and protein structure prediction, seeking insights on optimizing accuracy through advanced algorithms and models in computational molecular biology.
03 January 2024 9,099 0 View
How are microorganisms important in nutrient availability and transformation in the soil and role of microorganisms in nitrogen cycling in soils?
03 January 2024 7,370 3 View
Hello, I am attempting to co-express two different genes in Pichia pastoris X-33. My colleague successfully achieved this by transforming two different vectors simultaneously. Essentially, he...
02 January 2024 9,819 1 View
Everything is standardized as per protocol, but still this smearing is there, need suggestions from PCR experts. DNA extraction by the magnetic beads-based method. tried colony Pcr. PCR...
02 January 2024 6,866 4 View
These are the links of the two journals: https://gradivareview.com/ https://gradiva.it/home/ Thank you.
02 January 2024 1,622 2 View
Is there someone can download 'Targeted ADAMTS13 Replacement Therapy for Thrombotic Thrombocytopenic Purpura' DOI:https://doi.org/10.1016/j.jtha.2023.11.030 full text?
02 January 2024 2,861 0 View
as I am working first time on antibiotics for analysis of MIC by broth dilution method. I firstly prepared stock soln of 100mg/ml of ampicillin. now I want to ask how much ampicillin from its...
02 January 2024 8,473 2 View
I am working on a topic of adding dopants to the enamel of porcelain in order to enable its fluorescence under UV light. The problem is that I can't seem to find any research leads. What would you...
01 January 2024 5,770 4 View