Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
I'm working on some signalling assays using 96 well plates. For that, I want to measure the bioluminescence which generates from the sample. But in our lab, there is no luminometer. I want to...
23 May 2023 9,979 1 View
Hi, I am a beginner in bioinformatics and I would like to identify CRISPRs in my MAGs fasta files. Can someone recommend an up-to-date good tool that can be easily installed through the Conda...
22 May 2023 3,516 3 View
Hi everybody. I am a student working on a research project. I would like your help to gain a deep understanding of my work. I am using a mammalian expression system to synthesise and then purify...
22 May 2023 2,029 4 View
I have read the paper named"Isolating and engineering human antibodies using yeast surface display", in which described that the Nonimmune human scFv yeast library are available by request from...
19 May 2023 6,456 1 View
Hello dear researchers. After gel purification of the plasmid cut with restriction endonucleases, I cannot take measurements on a nanodrop. However, when I load the sample on an agarose gel, I can...
19 May 2023 843 3 View
I'm doing disc diffusion experiments with E. coli TOP10 carrying my plasmid. I noticed that there was no difference between the TOP10 carrying the empty vector (my control) and TOP10 carrying my...
19 May 2023 5,887 5 View
We were working on purification of a his-tagged protein from Expi-CHO cell pellet and tried different buffers along with sonication to get the protein out in the supernatant for further...
19 May 2023 7,669 5 View
My graduate work was in C. elegans, and this is my very first time performing live cell imaging in human cell lines. (fibroblasts) I would like to use live cell imaging to track two proteins of...
19 May 2023 1,082 2 View
I have done a plaque assay of hMPV-GFP1 twice for 4 days and 7 days and did not get plaques in those days. Could I get possible suggestions on how to achieve this? Thank you.
17 May 2023 4,893 0 View
I am trying to isolate high quality RNA from marine worms larvae using Direct-Zol RNA microprep from Zymo and the yield is very low. My biggest concern is the homogenization of the larvae once...
17 May 2023 8,407 0 View
Hello everyone! I recently received invitations to contribute an article to the current issue of the Journal of Brilliant Engineering (BEN) and Chemistry and Biochemistry. Can anyone with...
16 May 2023 9,393 2 View
Hello all I would like to start working with human iPSC cells, and looking for protocols that I can use it for thawing, freezing, regular passaging, and differentiate of this cell line to neuronal...
16 May 2023 7,729 2 View
I possess 250 peptides and one target protein, prompting my search for tools/servers that allow docking of multiple peptides simultaneously. While I have already utilized HPPdock, I am interested...
16 May 2023 9,755 2 View
I am an RA doing site-directed mutation in an 8.5kb plasmid; we use the Q5® Site-Directed Mutagenesis Kit from Biolab. I designed my primers in their tool Nebasechanger and followed every step in...
16 May 2023 228 9 View
I'm getting dark blue colonies and lawns when I'm plating E. coli cells transformed with plasmid containing E2 Crimson as the gene of interest under high copy number origin of replication and...
12 May 2023 7,731 2 View
I am analyzing the expression of a receptor tyrosine kinases protein and its phosphorylated form via Western blots. The phosphorylated form has decreased significantly, and the total remains...
12 May 2023 9,651 9 View
Does anyone know a method for generating a disordered protein ensemble with AlphaFold-Multimer? The protein I am interested in is disordered and is bound to a protein that is mostly folded. I know...
11 May 2023 6,097 1 View
Hello everyone. I have a question related to gene integration in the genome of Saccharomyces cerevisiae using a plasmid. Can anyone help me with the gene integration procedure in yeast using the...
11 May 2023 1,641 6 View
We are experiencing a problem in the DW region despite successfully amplifying the UP region in OE-PCR. The Tm value of the forward primer is 63.4 (excluding the overlap region), and the Tm value...
11 May 2023 8,656 7 View
I used the following procedure to transform DH10Bac E. coli. I wanted to know why my experiment failed. Exp. Procedure: 1. Thaw 25ul DH10Bac competent cells on the ice for each transformation. 2....
11 May 2023 9,452 3 View
Here is the situation: I usually culture 293FT cells in 10cm dishes and coat these dishes with 0.1% gelatin overnight to enhance adherence. When I passage these cells, seeding density: 1 millon...
10 May 2023 8,916 4 View
Dear All, For the environmental purposes and also saving money, can cell culture flasks be reused? I am using an adherent cell line. Whether T75or T175. Thanks!
08 May 2023 5,668 8 View
I need to select an appropriate plasmid to transform several different plant species. Ideally, I would like to use the same vector for all the species to limit variable differences and time. What...
08 May 2023 6,079 2 View
I have two linear DNA fragments that will be ligated together (each ~700 bp). This fragment then needs to be ligated into a linearised vector (plasmid). Would I need to perform a clean up on the...
08 May 2023 5,583 3 View