Is there any way to isolate RNA from Negative Pressure Wound Therapy (NPWT) samples?

16 January 2024 1 1K Report

I want to check mRNA expression levels in these NPWT samples. Is there any way to isolate RNA from these? I tried TriZol based extraction but all I got was a smear (considering the samples were thawed multiple times, I expected this).

Thank You

Margaret Bohmer

Multiple freeze-thaw cycles can cause the RNA in your samples to degrade. If the RNA is already degraded, then no extraction method will work. For your TriZol-extracted samples, are you able to see 28S and 18S bands? The 28S:18S ratio should be approximately 2:1.

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