Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
Hi, I have to perform corrections on my 8-band WorldView-3 imagery and only have ArcGIS Pro software available to me. Online I can only find information on how to perform these corrections in...
08 November 2023 6,208 1 View
Hello, i have to transport some primary mouse hepatocytes in some 6 wells plates at 37 degrees celsius ideally. It's going to be a 45 min drive approximately. Does anyone have any ideas or...
07 November 2023 4,026 1 View
In Applied Research Center, we have done research on some different genotypes of Linum usitatissimum and their characteristics in some different areas in the north of Iran. Now, we need some seeds...
07 November 2023 6,898 4 View
The step response of an MPC control system for a nonlinear plant gives a response with vertical line before it starts tracking. I have been trying to figure out what could be responsible for the...
06 November 2023 9,433 3 View
Are chelates generally more stable than the complexes and does the size of cheated rings affect the stability of complexes?
06 November 2023 1,893 3 View
PCR
03 November 2023 8,355 1 View
can someone guide me to the equation of SINGLE ATOM of any element? which equation that defines it's birth? Thanks
03 November 2023 8,779 10 View
How do you preserve bacterial culture for a long time and frequent a culture is subculture when stored at refrigerated condition?
03 November 2023 8,914 3 View
I have been following the IVT protocol as described in Henderson et al., “Cap 1 Messenger RNA Synthesis with Co-Transcriptional CleanCap® Analog by In Vitro Transcription.” This involves a 2.5hr...
03 November 2023 9,826 0 View
I'm currently using a Qiagen Kit for DNA extraction, I saw white precipitate in the P1 buffer, I was wondering if it's normal and will it effect my DNA yield ? Thank you in andvance
03 November 2023 4,242 4 View
Hello, I've been trying to isolate exosomes from cell culture supernatant. I have spoken to other researchers who do exosome work and found that they don't use RIPA buffer to lyse the eVs when...
02 November 2023 1,552 2 View
I need CEL1 enzyme to use it in my laboratory work to complete my PhD thesis. Unfortunately, I didn't find it anywhere. So I'm waiting for any request about companies name where is available or...
01 November 2023 275 3 View
Hi I was wondering whether CMV and EF-1alpha promoters can be put in a single plasmid? Are there any compatibility issues between the two promoters? Thank you.
01 November 2023 9,902 2 View
I have insert having flanking regions with XbaI site and and a vector digested with XbaI. How can I prevent self ligation in insert as well as vector before ligation?
01 November 2023 8,851 1 View
I am interested in viewing fatty acid treated vs untreated tissue to know if the fatty acid was taken by the whole tissue during treatment.
31 October 2023 5,517 2 View
Taking into account there are around 50 people affected, and it is necessary to act quickly to identify the pathogen
28 October 2023 5,168 1 View
please refer to me the protocol of insertion of PXR gene in existing DNA which has to be transfected in HEK293 WT cell line
28 October 2023 7,907 4 View
Antibakteriyel aktiviteye sahip bir ekstraktın antibiyofilm aktivitesine sahip olduğunu ve biyofilm oluşumunu azalttığını söyleyebilmek için bulunan değerin MIC değerinden düşük olması gerekir mi?...
28 October 2023 8,954 2 View
Do red blood cells participate in immunity?
28 October 2023 1,662 6 View
Hello, We have synthesized series of Hydroxyapatite nano-particles. We are trying to collect UV-Vis DRS spectra using Perkin Elmer - Lamda 750 spectrophotometer using integrating sphere. But we...
27 October 2023 8,130 7 View
I want to repair the missing amino acids of a protein structure and I cannot use Moddeller because there’s more than 20 residues to add, is there another option I can use?
27 October 2023 162 1 View
I am using a 40mm 1° cone with a 10% strain raised in 0.1s for 1000s to study the stress relaxation of hydrogels. The machine is HDR-1 TA Instrument. My issue arises when dealing with softer gels,...
26 October 2023 7,157 0 View
I am interested in studying the mechanisms of inhibition at the neuronal level and, above all, its disorders in psychiatric diseases. Unfortunately, I do not have access to MRI spectroscopy. I...
26 October 2023 7,744 0 View
Hello wonderful people out there, Could anyone please advise why BRIG keeps on generating the following error message while running the analysis: SYS_ERROR:The following error occurred:...
26 October 2023 9,236 2 View