I want to conduct haemolyis assay on mouse blood (I need 0,5 - 1 ml of blood), I know that it is generally hard to collect good sample without inducing haemolysis. As a anticoagulant do you prefer EDTA or heparin? Syrgine must be coated with anitcoagulant? And need I special tubes that are coated with anticoagulant or can I just prepare solution of anticoagulant in a tube? As I understand blood should be collected from heart or junglar vein? Do you have any recommendations?
Hi Alicja,
We collected blood from mice after euthanasia from the portal vein. It was a very long time ago, but I will try to remember the stages of blood sampling. First, 50-100 μl of heparin was drawn into the syringe, so that the solution was mainly in the needle. Immediately after cutting the abdominal cavity, the intestines were moved away, the vein that led to the liver was found, and a needle was carefully inserted into it. The needle should not be too thin to avoid hemolysis, the syringe plunger should move smoothly. After the blood had collected, the needle was removed and the blood was squeezed into the test tube.
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