Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
Hello everyone, I want to know how many cells are in my glycerol stocks. They were all made in the same way, and they yield similar results, but I kind of forgot about one step or two before...
07 December 2023 8,529 7 View
After 3 hours of exposure of formic acid under UV in the presence of BaWO4 and metallic silver and metallic nickel in the presence of BaWO4 and metallic silver and metallic nickel, I observed an...
06 December 2023 3,143 0 View
I have transformed NEB 5-alpha cells with a plasmid that I have pre-sequenced and confirmed it has the correct sequence. All I want to do is make more of this plasmid. However, when I transform...
05 December 2023 8,146 3 View
I need to evaluate viral accumulation by qPCR in plants with DNA and RNA virus coinfections . I dont know how proceed in tge sample treatment before the qPCR . Please someone has done this before ?
05 December 2023 3,635 0 View
Hello everyone! I bought a KOD HotStart DNA Pol, from Merck Millipore. By reading the specifications and datasheet i could notice that this enzyme is capable of amplifying up to 21kb of plasmid...
05 December 2023 8,095 2 View
Hi, In the last 3 months we are struggling with recurrent contaminations in our cell cultures. PCR results showed that the bacteria are Sphingopyxis sp. FD7 (by sequence alignment). Although I...
05 December 2023 5,661 2 View
I have been trying to perform phage purification (CsCl density centrifugation), DNA extraction (Norgen phage DNA isolation kit) and phage sequencing (MiSeq PE-150) for therapeutic phages targeting...
04 December 2023 2,697 5 View
Hello! I have a question regarding transfection stability in MCF7 and IMR32. For my experiment I need to transfect them with a plasmid with my gene of interest and Kan/Neo-gene which confers...
03 December 2023 1,594 2 View
Is there any manual method to precipitate PCR amplified product?
02 December 2023 5,519 4 View
I wanna find an enzyme that can cut single strand DNA at specific site efficiently, but I failed. Instead, I start to find an enzyme that can cut DNA hairpin structure which formed by single...
01 December 2023 1,150 0 View
Hi all, We have a lentiviral vector with a gene of interest that we would like to express. There is no requirement for stable cell line construction, so we will only be transfecting transiently....
30 November 2023 2,779 6 View
I'm doing T7E1 experiment to check CRISPR-Cas9 transfection efficiency. Although i see my PCR products in the true site on the agarose gel, i do not see any band when i do T7E1. It is as if no...
30 November 2023 797 3 View
Hello, I work with HaCaT cells, and until now there hasn’t been any issues. But recently I’ve had a problem when lysing the cells. Upon the addition of lysis buffer, the cell “suspension” turns...
29 November 2023 5,235 4 View
If yes, then how can I interpret the results?
28 November 2023 4,430 3 View
Hi everyone I have access to some HUVECs from LONZA. However, I am considering using an alternative media instead of Lonza EGM Endothelial Cell Growth Medium BulletKit. Has anyone ever used...
28 November 2023 6,113 4 View
I am currently working on the identification of phosphorylation on a specific protein, labeled as 'X,' from cell lysates. My approach involves immunoprecipitation using antibody 'X.' While this...
27 November 2023 1,788 2 View
I calculated SPSS that is better.
24 November 2023 3,399 5 View
Dear all, please comment about this. Thanks Letter: Bacteriophages and Colistin: A Promising Therapy and... Anionic Phage followed by cationic antimicrobial peptide Colistin Sequential Therapy...
24 November 2023 9,425 0 View
We need to find out the different active components in the mixture. A preliminary TLC run is necessary, but which solvent is the appropriate choice to proceed further?
23 November 2023 4,926 0 View
Im doing a qpcr for genotyping ERCC1 rs11615, now Im in a middle of optimization of Probe ERCC1 wild type, with 2 confirmed sample (WT and heterozygous sample). and the result, Ive got a big CT...
23 November 2023 9,958 4 View
I am working with Rota virus and I am using MA104 cell line for propagation. I am not able to differentiate between mock flask and infected flask as it involves the addition of trypsin for both...
23 November 2023 240 1 View
Hi all, I have been testing housekeeping gene stability in human dermal fibroblasts, ipsc and ipsc-derived cells for circadian studies. Using the same set of primers, the Ct for 18S rRNA is about...
23 November 2023 3,727 1 View
I have a 3.1 kb plasmid and I performed a digestion with the XmnI enzyme (37°C x 15 min and the enzyme was inactivated at 65°C x 20 min). When performing agarose gel electrophoresis, the size of...
22 November 2023 2,967 3 View
Hi dear researchers I need to calculate the insert mass for the ligation reaction, the vector length is 7800bp and the insert length is 83bp, would you please guide me what is the correct and...
22 November 2023 6,646 1 View