Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
Hello, I am currently working on making mutations in my plasmid conducting Quikchange. The plasmid size is 3800bp and I am using primers with several mutations. However, both my parental plasmid...
20 July 2023 5,148 3 View
Hello everyone, I am currently doing transient transfection. I want to transfect the overexpressed plasmid into HCC cells, and this plasmid has 10000+ base pairs. The transfection reagent I used...
16 July 2023 3,877 3 View
- Detailed steps to follow on how to upload the RNAseq data. - Type of format in which to upload it - Type of data to upload
16 July 2023 3,194 2 View
Does blue-green algae present in the soil fix gas into nitrogenous compounds and what will happen when Rhizobium bacteria is applied to the soil?
13 July 2023 5,348 0 View
I am busy generating a KO cell line with Jurkat suspension cells. After transfection I perform single cell dilution (limiting dilution) to approx. 70-100 cells per 96-well plate. It seems that...
12 July 2023 9,247 0 View
12 July 2023 4,912 5 View
Dear colleagues Please suggest a comprehensive and complete protocol for the production and purification of bacterial recombinant protein in E.Coli BL21 strain.
11 July 2023 8,274 2 View
I have a donor template with left and right homology arms (each 1000bp long) designed for a specific sgRNA cutting site but now I want to use another sgRNA cutting 15 bp downstream of the original...
11 July 2023 320 3 View
Hi everyone, I have been trying for months now to get my protein expression confirmed by western blot and failed every single time (I checked the antobodies, they are working fine). I have...
06 July 2023 6,917 5 View
These antibodies will be used to validate some antibodies by IHC. Thanks
06 July 2023 9,291 2 View
I am trying to transform pichia pastoris GS115, but when I run the negative control (the cells without the DNA) it is also growing in the selection medium Minimal Dextrose Medium:1.34% YNB 4 ×...
05 July 2023 917 6 View
Hi, does anyone know of a database or method for predicting the expression of a recombinant protein in E. Coli which strain of E. coli is better to have the highest soluble expression of the...
05 July 2023 2,572 4 View
I attached a picture of a blot with intense nonspecific staining at the bottom of the nitrocellulose blot. The left blot, from left to right, shows a ladder, a 20ug load and a 40 ug load. The...
05 July 2023 8,325 4 View
For solving chemical parameters of a single crystal, which softwares are helpful, in addition to that, which is good for doing docking analysis
05 July 2023 436 4 View
Hello everyone, I would like to know if someone already used the pRSET plasmid into normal BL21(DE3) and not into BL21(DE3)pLysS for protein expression. I'm having some induction and purification...
04 July 2023 6,851 3 View
I am currently using NEBuilder Hifi DNA assembly to perform Gibson Assembly on several DNA fragments. The assembled fragment is okay, which is checked by gel electrophoresis. However, when I...
04 July 2023 1,433 1 View
Hello, currently, I am working on the chloroplast genome and experiencing difficulties in reading ambiguous code N in the intron. I have blasted it to NCBI, but there are no nucleotides aligned...
03 July 2023 1,699 0 View
I searched and it is not in https://beallslist.net/standalone-journals. I need guidance on it please.
02 July 2023 9,968 3 View
We have purchased recombinant protein in the form of dimers, trimers and oligomers (confirmed by carrying out non reducing gels). In order for successful ligand binding assays we anticipate that...
30 June 2023 4,904 3 View
I sent a gene i recovered from gel after I amplified the gene from the plasmid extracted from metagenomic library by degenerative primers to a sequencing company but the sequencing failed several...
30 June 2023 7,577 4 View
My possitive control for transformation with original plasmid worked well and obtained colonies.
28 June 2023 3,745 5 View
First, there are bubbles with small black dots inside visible. The next day those bubbles disappear and the rod-like structures are present in our cell culture, which are fast growing but not...
27 June 2023 4,135 1 View
Good day everyone! Please I want to do transfection and Lentivirus packaging, and also lentivirus infection on my cell lines 293FT cells, how do it. Please i need a step by step guide, I will be...
23 June 2023 9,821 2 View
I have two plasmids, one as an indicator. The colonies show green or white but after streaking on a new plate they give mixed color colonies. The colonies are far away from each other in the first...
22 June 2023 5,478 4 View