Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
Hi, I’m working with peripheral blood samples from patients with nephrotic syndrome to extract genomic DNA. I’m using a commercial kit based on cell lysis, proteinase K digestion, and ethanol...
29 July 2025 6,675 0 View
I have done repeated trials according to the protocol mentioned in the kit literature; however, I am unable to get a standard curve as expected. Can anyone help or guide in this regard?
29 July 2025 3,406 0 View
I'm trying to figure out the methodology according to the American Pharmacopeia to use in non-sterile cosmetic assays. If I dilute 10g of my sample with a neutralizing broth, letheen in this...
28 July 2025 5,107 3 View
I want to draw metal layered double hydroxide and other layered materials for which I need a software apart from Chemdraw.
16 July 2025 8,138 0 View
I have a question about leaky protein expression. Our lab has stored GFP plasmids. Previously, when we transformed this plasmid into E. coli, there was no leaky expression. However, after a recent...
09 June 2025 6,971 2 View
I have prepared Chitosan methacrylate. After its preparation when I tried to dissolve and mix PEGDMA to UV crosslink using IRGACURE, no crosslinking is observed. Can anyone find me a reason for it?
29 May 2025 3,382 1 View
not significant
27 May 2025 1,779 1 View
I need to match some vaccines epitopes and see similarities
17 May 2025 411 1 View
After extensive research, I found information only on the pGro7 plasmid. However, the inducing agents for pGro7 and pGro12 differ. I aim to use IPTG for protein expression, and literature...
03 May 2025 8,675 1 View
Hello, I was wondering if it is possible to detect a modified nanoparticle (5nm diameter) in the binding site of my protein using X-ray crystallography. The protein I want to co-crystallise with...
29 April 2025 3,512 1 View
For PCA interpretation i need a research paper
24 April 2025 1,759 0 View
In the ODTBT model, recursive coherence builds across nested holonic scales until a threshold, TWIST, is reached. Recent research on the median raphe nucleus (MRN), interpeduncular nucleus (IPN),...
23 April 2025 1,126 0 View
There is growing conversation nowadays on Shannon's Entropy. These answers may add to the thickening of this discourse.
22 April 2025 2,034 20 View
I’m exploring whether negentropic entanglement, conceived as the anti-entropic process through which informational order arises from quantum-thermal or functional fluctuations, has been...
18 April 2025 7,650 0 View
I’m investigating the theoretical proposal that time in the brain may not be strictly linear or metric, but instead emerges as a nonlinear thermodynamic field, one shaped by entropy gradients,...
18 April 2025 416 0 View
Hi, we have Shimadzu UV 2600i UV-Vis-NIR spectrophotometer in our lab. But when we are trying to get the absorbance of Glass/Mo sample it is showing erronious results though for the baseline, we...
17 April 2025 6,474 3 View
I am analyzing protein–ligand interactions and used Discovery Studio Visualizer (DSV) to identify various non-covalent interactions, such as π–π stacking, cation–π, and π–alkyl interactions. I...
05 April 2025 356 1 View
How do we design fungal and plant gene cloning primers using BamHI and SalI restriction sites? What other additional sequence sites should be included and what about the gene sequences? Should we...
27 March 2025 9,004 2 View
This question relates to my latest preprint excerpt: Logical Inference using Bayesian Networks (ProbabilisticLogic.AI) My contribution is a closed form version of Symbolic Probabilistic...
22 March 2025 5,933 4 View
while the long middle part cannot matched but still there are colonies in the plate and can be cultured. What happened to this phenomenon and how can we solve it?
21 March 2025 1,272 0 View
I transfected COS-7 cells with my plasmids of interest and performed co-IP using mouse anti-GFP for IP and mouse anti-myc for IB. I got positive results, confirming that my proteins of interest...
15 March 2025 9,262 3 View
Is it possible to predict by bioinformatic tools if a protein is substrate of a kinase?
08 March 2025 753 9 View
I want to do an EMSA using an RNA sequence of 30/40 bp that forms an hairpin in the 3' end. I wanted to avoid to labeled it with radioactivity so I wanted to try the Chemiluminescent assay in...
04 March 2025 6,766 1 View
I'm currently using the LUMOS II FTIR microscope to analyze the chemical composition of microplastic particles. But recently, I keep getting the same spectrum (see image), whether on different...
26 February 2025 9,009 3 View