Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
As title describes, we're trying to mutate an amino acid of antibody Fc to cysteine, followed by TCEP to further drug conjugation, one of my colleague had raise the concern of purified TCEP...
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Hello! I´m developing a sandwich ELISA. My assay recognizes protein denaturalized very well, but there isn´t recognition when it is in its native form. So maybe if I heat the sample I could detect...
19 April 2024 5,654 3 View
For my project I am looking for the mRNA sequences of mRNA vaccines that are currently undergoing clinical trails or that are already in use. I know that the sequences of two Covid-19 vaccines are...
17 April 2024 619 1 View
what experience does women with trauma go through?
17 April 2024 4,387 0 View
In critical care settings, how does the implementation of virtual simulation technology compared to traditional training methods affect nursing staff's confidence, competencies, and...
11 April 2024 4,489 0 View
The principles of airway management in paediatric anaesthesia are crucial for ensuring adequate oxygenation, ventilation, and prevention of complications during surgery.
10 April 2024 7,713 1 View
as viruses are notorious for being prone to mutation at high rates its very difficult to clone them though general cloning procedures and cells so in order to solve this problem need specific cell...
04 April 2024 9,005 5 View
I have an error message, having removed a file of my latest publication which contained a small change. I have been trying to upload the new version without success. Underneath the PDF file...
04 April 2024 9,482 1 View
I have prepared competent cells of L. lactis NZ9000. But transformation not working. No colony at all in the plate. Parameters are as following: plasmid concentration: 1 uL (300ng) Electroporator-...
03 April 2024 1,154 3 View
Dear all, I am currently working on Acinetobacter baumannii and I wanted to look for the RBS or Shine Dalgano sequence of a gene for cloning. I have tried searching prediction online tools like...
02 April 2024 9,730 0 View
Dear Scientists, I have noticed that some stem cell companies are promoting the use of UCMSC/WJMSC in passage 2 and claiming that it is superior to passages 3-5, which are commonly used in...
30 March 2024 6,931 2 View
Hello everyone, I've been trying to clone a bacterial protein from S. aureus into E. coli with pQE30 vector. After ligation and transformation (with Xl1Blue), I screened my colonies (more than...
28 March 2024 1,585 3 View
Hi I have a few oligonucleotides with G4 forming sequences that I have checked they form G4 by CD spectroscopy. I plan to double check G4 formation by using Thioflavin T (ThT) fluorescence...
28 March 2024 4,240 0 View
After completing DNA extraction from whole blood using the QIAamp DNA Mini kit but obtaining no yield, I suspect the DNA might be retained in the column. How can I recover the DNA from the column.
27 March 2024 9,099 2 View
can we use crop drought tolerance indexes for conifers as well?
26 March 2024 6,352 1 View
Why does phosphorus not circulate in the atmosphere to a great extent and what happens when too much phosphorus is in a freshwater ecosystem?
19 March 2024 9,948 3 View
Hello, I have measured the optical density at 600 nm of a bacterial suspension. I got a value of 1.65. I then diluted the suspension 1:10 in medium and got a measurement of 0.144. What would be...
15 March 2024 2,558 1 View
I am currently conducting an analysis on a wind turbine and I am trying to input periodic boundary conditions in the mesh and it keeps showing me the same error which is the meshing algorithm...
14 March 2024 9,807 0 View
I cloned 3HR and 5HR of p.falciparum on a plasmid and the next step is gRNA cloning. I did colony PCR for verification it is perfect but the thing is after taking the toothpeak of the colony in...
13 March 2024 5,695 2 View
I have isolated Acidothiobacillus thiooxidans from environmental rust samples using ATCC 9K media. The pH of 9K media varies between 2.5-3.0, making a highly acidic environment for bacteria to...
12 March 2024 7,610 2 View
What microorganisms decompose dead organic waste of plants and animals and role of microbes in environmental bioremediation?
10 March 2024 2,496 0 View
How to calculate the concentration load of any constituent on the downstream side of a river or canal?
26 February 2024 2,758 3 View
paper
22 February 2024 4,584 2 View
I'm trying to isolate Lysosome using a percoll (sigma, cat# 4937) gradient of 57.6%, 43.2%, 28.8% and 20.2% (Ref: Lysosomal and non-lysosomal peptidyl hydrolases of the bloodstream forms of...
22 February 2024 1,013 1 View