Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
Hi, I am using Strep-Tactin® Superflow® high capacity resin by IBA to purify a Twin-Strep tagged fusion protein at N-terminal. I am facing issue to elute out the protein. For elution I have...
31 December 2023 5,217 1 View
Heliyon does not give a clear format for the reference. Which should I choose in Endnote?
29 December 2023 1,765 1 View
Do microorganisms have relationships with plants and how do different types of microorganisms found in soil contribute to the health and productivity of plants?
29 December 2023 3,424 3 View
The only answer I have come close to is the "hidden break" event of ribosomal subunits reported in mollusks and insects. However, in these studies up to 3~4 bands are reported, in my case I have...
28 December 2023 1,099 0 View
Navara rice which is the traditional rice from kerala
28 December 2023 7,525 1 View
I want convert the Titer of Virus which is given in 0.5ml to 1mL. tired many times but cant find a solution
27 December 2023 8,338 3 View
In CRISPR/Cas, we design guide to make double strand break in a specific site of the genomic DNA. I am curious to know, whether the gRNA-Cas9 complex has any possibility to bind to the messenger...
26 December 2023 605 1 View
Assuming the electrical breakdown probability follows the two-parameter Weibull distribution function: 📷 F(E): the failure rate; E: electric field (MV/m); β: shape factor; α: Electrical...
26 December 2023 1,736 0 View
Hello! I am doing a CRISPRi-screening. I prepared sequencing library by PCR amplification using a i5 common primer and i7 primers with single TruSeq index, and performed a paired sequencing on...
25 December 2023 6,423 1 View
Hi I isolated RNA samples from cells and made cDNA. In the case of troubleshooting, I would like to validate my RNA sample by looking at 18s and 28s. but I don't know how to do it. I have...
25 December 2023 4,496 1 View
Does anyone know how I can download this white paper?: Mapping the genome of jobs: the Burning Glass skills taxonomy. I am not able to find it after thorough research.
23 December 2023 3,434 0 View
Dear Researchers, We are seeking participants to collaborate on writing an article and report on the utilization of different artificial intelligence (AI) tools in healthcare care plan...
23 December 2023 5,195 8 View
t is used to compare two variables only and is parametric As for the chi-square, it is for comparison between groups and is non-parametric. Is this true?
23 December 2023 9,601 6 View
Please give complete recepie and preparation protocol
23 December 2023 3,020 0 View
Hi I have be studying on bone derived MSC and in the begining I did passaging, ICC, freezing cells and defrosting cells. There was no problem but now my MSC cell cultures were infected two times...
22 December 2023 1,258 2 View
Hi how can I upload a manuscript for a paper that wasn't uploaded before thanks?
22 December 2023 7,762 0 View
I want to synthesize Gene-specific real-time PCR primers for my differentially expressed novel and conserved micro RNAs in plants.
22 December 2023 5,068 3 View
Recently I conducted the small RNA sequencing of extracellular vesicles (EV) from a donor cell line and found several highly expressed miRNAs. Therefore, I tried to check the expression of them in...
21 December 2023 2,927 0 View
Meiotic nondisjunction of chromosome 21 is a common reason in case of Down syndrome. This susceptibility to nondisjunction is found to be increased in women older than 45 years of age.
20 December 2023 8,369 3 View
Are there phage preparations for phage therapy against Yersinia enterocolitica? Maybe from Georgia? Thanks
19 December 2023 2,673 3 View
From my frozen salmon kidney samples (stored at -80C), I have confirmed the presence of target bacteria using PCR. However, I stored the sample after the experiment. Are there any methods or ways...
19 December 2023 3,591 6 View
I have used Trizol method but there has been huge DNA contamination along with shearing.
18 December 2023 4,728 4 View
I prepared 20 gm TEA, 30 gm water and 50 gm Boric acid but it not soluble , it becomes thick hazy.
18 December 2023 2,118 5 View
Can you please explain to me what exactly it means when the % of mediated total effect in a mediation model is larger than 100%. I have a model with inconsistent mediation (or supression). My X...
18 December 2023 2,273 0 View