Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
what the function of satellite that found in centromere of chromosome????
07 May 2023 1,866 0 View
Hi I need to make sure of the journal that I want to submit a manuscript is not fake. I have the journal citation report for 2022 but I need a journal citation report for 2023. regards
04 May 2023 9,863 3 View
Hi . I am a student researcher and I am writing this to request your help in obtaining Top 10 cells and expression plasmid for my research experiment. Unfortunately, my current budget does not...
03 May 2023 650 4 View
I am purifying my L-Asparaginase (with 6 his-tag) using Ni-nta chromatography, and imidazole 500 mM + potassium phosphate 0.1 M as and elution buffer and pH of 7.5, and then I check the activity...
02 May 2023 7,264 11 View
I am starting with 1.15 ug of both vector and insert for double digestion. But after gel elution I am only able to get around 3.7 ng/ul; and 10 ng/ul for vector and insert respectively in 15ul of...
29 April 2023 5,451 3 View
Hello! I am using T7-mcherry plasmid in E. coli BL21(DE3). When I streak it on LBAmp plate, the colonies show beautiful purple-red color. When I streak it on LBAmp+0.1mM IPTG plate, the colonies...
27 April 2023 6,901 2 View
I want the manual of molecular cell biology by Bruse Albert
27 April 2023 3,360 3 View
Needs guidance
27 April 2023 5,021 1 View
Hello I am trying to cut a segment from a plasmid that is around 80 and I wonder if I could see it on the gel taking in consideration that the smallest size on the ladder is 100 bp. however, I...
26 April 2023 9,341 3 View
I'm looking for a freely available software for pharmacophore based virtual screening.
26 April 2023 3,492 4 View
Hi all, I have transfected LentiX293 T cells with a plasmid expressing TagBFP under mouse PGK promoter. However, after checking under microscope, I can not see any signal. Can having a mouse...
25 April 2023 2,604 4 View
My plasmid for site-directed mutagenesis id loosing my gene of interest. Is there any way to dill with this problem?
25 April 2023 3,821 3 View
We recently carried out FAME (Fatty Acid Methyl Ester) experiment. We have the raw data but our Midi software is not updated. Are there any softwares where we can analyse our FAME data for...
22 April 2023 8,262 1 View
I want to purchase Macbook mainly for the bioinformatics analysis propose i.e., Transcriptomics, smalRNA, Methylation, lncRNA and other. Would anyone please suggest to me the best affordable one?
21 April 2023 7,183 3 View
Good day all I am having difficulty with my agrobacterium transformations with regard to agro overgrowth after co-cultivation. I am currently using 100 mg/L timentin along with 400 mg/L...
20 April 2023 1,078 7 View
I want to know that, when a heterologous gene is expressed under CMV promoter in mammalian cells, what is the percentage of this heterologous gene's mRNA in comparison to total cellular RNA and...
19 April 2023 5,954 2 View
This a ligation PCR, which I'm doing it to check if my ligation worked or not. I am using two primer pairs with very similar annealing temperatures. Ideally, if the ligation worked it should...
17 April 2023 3,064 2 View
Hi! I have trouble with the SDS-PAGE. I do not know why my protein band and size marker are not visible in the high molecular weight above 50 kDa. At this moment, I have tried to change the...
15 April 2023 3,600 13 View
I tried graphpad (trial version) and it is great , just what i need. But is not free and no possibility if buying license . So, does anybody knows a free alternative? . I have no experience with...
15 April 2023 3,216 1 View
Hi all! I am trying to construct a genomic DNA library of a gram-positive bacterium. I have isolated the genomic DNA and now I have to chop off the DNA into small fragments for cloning into a...
15 April 2023 4,002 5 View
I am making the following formulation for oral gavage in mice, 20% PEG 400 + 10% Cremophor + 70% PBS. The solution is viscous and cloudy. Is this normal?
14 April 2023 2,571 3 View
I have isolated genomic DNA from Klebsiella pneumoniae using promega wizard bacterial genomic DNA extraction kit. I have followed the protocol instructions. But I can see smears in my gel and...
14 April 2023 8,394 2 View
We are evaluating to sequence some plasmids using this company service (https://www.plasmidsaurus.com/index/). We want to contact with any spanish lab which has a succesful experience in send DNA...
14 April 2023 3,367 3 View
Which is the best protocol for the good quality genomic DNA extraction from the bacteria for nanopore sequencing other than commercial kits.?
14 April 2023 7,874 8 View