Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a... | Contact experts in Plasmids to get answers
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Questions related to Plasmids
Hi. I am involved in a project in the area of gastric cancer and I should make a culture from KATO III cell line. I revived my stock. But after 48 hours I saw apoptotic cells. The culture media,...
18 December 2023 9,958 5 View
If we do not have access to an ultracentrifuge and PEG is lethal to our target cells and has a highly toxic effect, the articles mention the use of lentivirus concentration columns. Does anyone...
17 December 2023 8,976 0 View
I humbly wish to seek for assistance from molecular biology an bioinformatics experts to get a novel research topics that can be used as seminar and thesis in my masters program. thanks in...
16 December 2023 1,559 3 View
Can multiple instances of the same ligand be docked to one macromolecule? For instance, can one ligand be docked and then the output used as input for a second docking of the same ligand, and so on?
15 December 2023 4,120 4 View
My situation is: I used MRI-compatible EEG electrodes, and all the participants went through a 3D T1-weighted brain image scan with the EEG electrodes on the scalp. Is there any toolbox that I...
15 December 2023 8,061 1 View
I am attempting to perform disk diffusion assays with novel extracts but the blank disks I use (remel, processed blank disks) are producing small zones of inhibition. I have tried using disks from...
15 December 2023 5,201 0 View
The cDNA was amplified by Q5 and PCR product was analyzed by agarose gel electrophoresis. Would cDNA template degradation cause such smeared or smiling bands?
14 December 2023 3,015 12 View
Green synthesis procedure Plant extract mixed with solver nitrate solution and placed under sunlight for 3 hrs
14 December 2023 8,286 2 View
Hello everyone, In one of our studies we need to investigate the dielectric constant of material for cables. The NAYY cables have 4 different parts in frequency from 1MHz to 40MHz. The first one...
14 December 2023 6,046 3 View
I inoculated 20 mL of Rosetta pre-culture into 1L LB broth supplemented with 1 mL of Kanamycin and 1 mL of Chloramphenicol. After one hour of incubation, I checked the OD, and the value observed...
14 December 2023 4,902 1 View
The attached table below shows the zone of inhibition (ZOI) in mm of: 1. Rutin 2. Heliomycin 3. AgNPs(silver nanoparticles) 4. AgNPs + Rutin 5. AgNPs + Heliomycin 6. Tetracycline (positive...
14 December 2023 2,546 9 View
Hello, To check specificity of your primers by ncbi blast, we find identity percentage, query cover and E-value as the parameter to be taken care off. I know that query cover is important but i...
14 December 2023 6,973 1 View
In the past few years, the activity of the metalloproteinase expressed by bacteria was very low, but recently several expressions have suddenly shown an increase in activity. The used Plasmid...
13 December 2023 4,728 6 View
I'm curious about the current scientific understanding regarding their existence and function within bacterial systems.
13 December 2023 6,460 0 View
I have been trying to express my construct for the last couple of weeks but after several attempts, it's still failing although similar parts (five inserts for golden gate assembly) have been used...
13 December 2023 4,703 8 View
I am a master's student in Korea. I have some questions while conducting a cell culture experiment on PDMS. 1. What should I do to allow HaCaT cells to grow on PDMS without O2 plasma treatment...
13 December 2023 5,536 0 View
Lentiviruses are often used as delivery vectors for in vivo crispr screening because the lentiviral genome is able to integrate into the cellular genome, so is it okay to use AAV, a virus that...
12 December 2023 7,723 0 View
The cells do not confluency after thawing. Even after 10 days, the color of the medium does not change. I change the medium, the non-adherent cells disappear and then the density in the flask...
12 December 2023 6,986 2 View
Hello everyone, I want to perform western blotting and immunofluorescence on macrophages isolated from mouse lung. I tried cell sorting in FACS with negative staining but I did not harvested...
12 December 2023 3,244 0 View
How to download the amino acid sequence of a gene from all Arabidopsis ecotypes/accession from 1001 Genomes or any other database?
12 December 2023 1,010 7 View
Hello, I am looking for genomic DNA isolation protocol which does not involve centrifugation process. I want to isolate genomic DNA from oral swab/sputum sample. I will appreciate your valuable...
11 December 2023 8,713 3 View
I am working on Artificial dermal matrix project and I have extracted collagen, I want to incorporate the synthetic elastin or tropoelastin in the collagen I extracted so that the dermal matrix is...
11 December 2023 7,137 1 View
Recently, I installed Modeller 10.4 software into my windows 10, 10GB RAM, 64x bit laptop to predict a 3D structure of a membrane protein (a.a length 574). In this case , i used advanced modeller...
10 December 2023 2,331 2 View
I have been trying to digest a vector pXMCS for the last two months but no luck so far. I have been using NdeI and KpnI (Both of them were present on the plasmid map of the vector) as restriction...
07 December 2023 2,677 3 View